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产品名/货号/品牌 简介 包装  
蛋白酶 K,分子生物学级 :Proteinase K, Molecular Biology Grade 2 ml

价格:¥

货号:P8107S
品牌:NEB

经销:基因有限公司

蛋白酶 K(分子生物学级)2ml。蛋白酶 K 是一种枯草杆菌蛋白酶相关的丝氨酸蛋白酶,可水解各种肽键。蛋白酶 K 的活性范围宽泛,在温度 20 - 60℃ 和缓冲液 pH 7.5 -12.0 的范围内具有最佳活性状态(1,2)。当反应中含有高于 2% SDS 和 4 M 尿素时活性受到影响(3)。钙的存在对蛋白酶 K 的热稳定性很重要,而与其催化反应无直接关系,因此蛋白酶 K 在含有螯合剂(如 EDTA )的反应缓冲液中仍保持活性(4)
蛋白酶 K 是一种枯草杆菌蛋白酶相关的丝氨酸蛋白酶,可水解各种肽键。

2.5% Trypsin-EDTA Solution 10X:2.5% Trypsin-EDTA Solution 10X 100 ml

价格:¥

货号:sc-391060
品牌:Santa Cruz

经销:基因有限公司

2.5% Trypsin-EDTA Solution 10X is a concentrated solution that can be diluted to the 1x working concentation. This product is made from trypsin powder (1:250) extracted from porcine pancreas.

2.5%胰酶-EDTA Solution 10X:2.5% Trypsin-EDTA Solution 10X 1 L

价格:¥

货号:sc-391060A
品牌:Santa Cruz

经销:基因有限公司

胰酶-EDTA:Trypsin-Versene (EDTA) 100ml

价格:¥

货号:17-161E
品牌:Lonza

经销:基因有限公司

Trypsin-EDTA (ethylenediaminetetraacetic acid) is a combined reagent used to remove cells from the culture vessel surface into suspension. The EDTA, a chelator of divalent cations, is added to trypsin solutions to enhance activity. The calcium and magnesium in the extracellular matrix, which aids in cell-cell adhesion, also obscures the peptide bonds that trypsin acts on. The EDTA is added to remove the calcium and magnesium from the cell surface which allows trypsin to hydrolyze specific peptide bonds. This activity can be halted by adding a serum media mixture, or a Trypsin inhibitor (from soybean, for example) in serum-free systems. It is supplied at use dilution. 17-161 contains 0.5 grams per Liter Trypsin 1:250, 0.2 grams per liter Versene (EDTA). Porcine parvovirus tested and mycoplasma screened. Product is manufactured with irradiated trypsin.

胰酶:Trypsin 100ml

价格:¥

货号:17-160E
品牌:Lonza

经销:基因有限公司

a clear, colorless-yellow, highly active porcine enzyme preparation, used to remove cells from the culture vessel surface into suspension. It is generally used at a working concentration of 0.25%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8. After treatment, trypsin activity can be halted by adding a serum media mixture or a Trypsin inhibitor (from soybean, for an example) in serum-free systems.

胰酶-EDTA:Trypsin-Versene (EDTA) 500ml

价格:¥

货号:17-161F
品牌:Lonza

经销:基因有限公司

Trypsin-EDTA (ethylenediaminetetraacetic acid) is a combined reagent used to remove cells from the culture vessel surface into suspension. The EDTA, a chelator of divalent cations, is added to trypsin solutions to enhance activity. The calcium and magnesium in the extracellular matrix, which aids in cell-cell adhesion, also obscures the peptide bonds that trypsin acts on. The EDTA is added to remove the calcium and magnesium from the cell surface which allows trypsin to hydrolyze specific peptide bonds. This activity can be halted by adding a serum media mixture, or a Trypsin inhibitor (from soybean, for example) in serum-free systems. It is supplied at use dilution. 17-161 contains 0.5 grams per Liter Trypsin 1:250, 0.2 grams per liter Versene (EDTA). Porcine parvovirus tested and mycoplasma screened. Product is manufactured with irradiated trypsin.

胰酶终止液:Trypsin Neutralizing Solution 100ml

价格:¥

货号:CC-5002
品牌:Lonza

经销:基因有限公司

胰酶/EDTA 溶液:Trypsin/EDTA Solution 100ml

价格:¥

货号:CC-5012
品牌:Lonza

经销:基因有限公司

间充质干细胞用胰酶/EDTA:Trypsin/EDTA for MSC 100ml

价格:¥

货号:CC-3232
品牌:Lonza

经销:基因有限公司

Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), rat bone marrow derived mesenchymal stem cells (rMSC), and dental pulp stem cells (DPSC).

胰酶:Trypsin 500ml

价格:¥

货号:17-160F
品牌:Lonza

经销:基因有限公司

a clear, colorless-yellow, highly active porcine enzyme preparation, used to remove cells from the culture vessel surface into suspension. It is generally used at a working concentration of 0.25%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8. After treatment, trypsin activity can be halted by adding a serum media mixture or a Trypsin inhibitor (from soybean, for an example) in serum-free systems.

蛋白酶 K:Proteinase K 100mg

价格:¥

货号:740506
品牌:MNG

经销:基因有限公司

蛋白酶K溶液:Proteinase K Solution 5ml @ 20mg/ml

价格:¥

货号:BIO-37084
品牌:Bioline

经销:基因有限公司

蛋白酶K溶液(5ml,20mg/ml),核酸纯化辅助产品 蛋白酶K溶液,20mg/ml。分子级纯度。A highly active serine protease with broad cleavage specificity on native and denatured proteins. (MW 28,500 Da) Isolated from the fungus Tritirachium album. Free of detectable DNase and RNase. Especially suitable for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100µg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

High activity - broad-spectrum serine protease
Active under denaturing conditions - useful for a variety of applications
Stable - at high temperatures
Molecular biology grade - useful for general digestion of proteins
Proteinase K is also available in powder form

Applications
Inactivation of RNases/DNases during nucleic acid extraction
Protein modification
General protein digestion
Determination of enzyme localization
Remove nucleases for in situ hybridization
Improving cloning efficiency of PCR products

蛋白酶K:Proteinase K 100mg

价格:¥

货号:BIO-37037
品牌:Bioline

经销:基因有限公司

蛋白酶K,100mg,核酸纯化辅助产品 A highly active serine protease with broad cleavage specificity on native and denatured proteins. (MW 28,500 Da) Isolated from the fungus Tritirachium album, the enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Free of detectable DNase and RNase. Ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100μg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

High activity - broad-spectrum serine protease
Active under denaturing conditions - useful for a variety of applications
Stable - at high temperatures
Molecular biology grade - useful for general digestion of proteins
Proteinase K is also available as a stabilized stock solution
Applications Inactivation of RNases/DNases during nucleic acid extraction; Protein modification; General protein digestion; Determination of enzyme localization; Remove nucleases for in situ hybridization; Improving cloning efficiency of PCR products.

蛋白酶K溶液:Proteinase K Solution 5 x 5ml @ 20mg/ml

价格:¥

货号:BIO-37085
品牌:Bioline

经销:基因有限公司

蛋白酶K溶液,20mg/ml。分子级纯度。A highly active serine protease with broad cleavage specificity on native and denatured proteins. (MW 28,500 Da) Isolated from the fungus Tritirachium album. Free of detectable DNase and RNase. Especially suitable for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100µg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

High activity - broad-spectrum serine protease
Active under denaturing conditions - useful for a variety of applications
Stable - at high temperatures
Molecular biology grade - useful for general digestion of proteins
Proteinase K is also available in powder form

Applications
Inactivation of RNases/DNases during nucleic acid extraction
Protein modification
General protein digestion
Determination of enzyme localization
Remove nucleases for in situ hybridization
Improving cloning efficiency of PCR products

蛋白酶K:Proteinase K 1000mg

价格:¥

货号:BIO-37039
品牌:Bioline

经销:基因有限公司

A highly active serine protease with broad cleavage specificity on native and denatured proteins. (MW 28,500 Da) Isolated from the fungus Tritirachium album, the enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Free of detectable DNase and RNase. Ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100μg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

High activity - broad-spectrum serine protease
Active under denaturing conditions - useful for a variety of applications
Stable - at high temperatures
Molecular biology grade - useful for general digestion of proteins
Proteinase K is also available as a stabilized stock solution
Applications Inactivation of RNases/DNases during nucleic acid extraction; Protein modification; General protein digestion; Determination of enzyme localization; Remove nucleases for in situ hybridization; Improving cloning efficiency of PCR products.

蛋白酶K:Proteinase K 100mg

价格:¥

货号:76230Y100MG
品牌:USB

经销:基因有限公司

Proteinase K 冻干粉,源于白色链球菌,20~ 50 units/mg,

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