收藏生物通商城 您好!欢迎来到生物通商城![请登录],新用户?[免费注册] 立即咨询 QQ 点击这里给我发消息 我的订单 帮助

商品比较

抗体搜索:
生物通商城首页 -> 试剂 > 免疫学 > Western Blot免疫印迹 > WB发光检测

免疫学

一抗

二抗及Ig结合蛋白

抗体组合

免疫组化

Western Blot免疫印迹

免疫沉淀

ELISA

ELISPOT

AlphaLISA

AlphaScreen/互作分析

LANCE(TR-FRET)

多重细胞因子检测

DELFIA TRF检测

流式

同型对照

免疫对照/血清/阻断/封闭

诊断相关血清

封闭剂

免疫缓冲剂/固定/透化

细胞裂解/组织抽提液

染色前处理/Mount

生物素/BAS系统

半抗原

抗体纯化

  • 促销专题
  • 您浏览过的产品

清除历史

商品筛选

种类:
不限化学发光底物化学发光检测试剂盒
品牌:
不限 CST PerkinElmer Santa Cruz Vazyme
抗体筛选:
筛选:
简介:
分类简介:

化学发光检测已经成为western blots检测中最好和最全面的检测方法之一。它减少了放射性物质和有毒显色基质相关的危害,其检测速度和灵敏度的都是传统方法无可比拟的。二抗上偶联的辣根过氧化物酶(HRP)与化学发光的底物特异性反应生成光信号,可被胶片或者仪器记录,生成在胶片上的实验结果可以永久记录和保存。不同的发光底物所产生的发光强度和持续时间不同,使得其灵敏度不同。ECL是最为熟知和经典的发光底物。辣根过氧化物酶与抗体的轭合物有非常高的周转率,在较短的时间里有很高的敏感性。化学发光方法检测出的印迹可以很容易被洗脱并且易于采用其它抗体重新检测。

  • 排序
  • 价格
  • 关注度
  • 销量
  • 更新时间
  • 第1 / 1页 共28条 上一页 下一页
产品名/货号/品牌 简介 包装  
Western Blotting Luminol Reagent:Western Blotting Luminol Reagent 1 kit

价格:¥

货号:sc-2048
品牌:Santa Cruz

经销:基因有限公司

Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.Extended exposure times (multiple exposures) to film made possible by long-duration signalWestern blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.

SignalFire™ ECL Reagent 50ml

价格:¥

货号:6883P3
品牌:CST

经销:基因有限公司

增强的ECL化学发光检测试剂,50ml。具有极高的敏感性,可以检测低达皮克级水平的蛋白。与其他的发光系统相比较, SignalFire™ ECL 试剂可以产生更强的信号,可以更长时间的输出信号。底物暴露于HRP后,光发射立即达到最大并且可以持续0.5-1小时。光可以被记录在胶片上,一般暴露几秒钟。延长暴露时间可以增加灵敏度。
50ml(25ml each substrate). An enhanced luminol based system designed for use with CST™ HRP-linked secondary antibodies and Phototope®-HRP detection assays. SignalFire™ ECL Reagent is extremely sensitive and capable of detecting protein levels at low picogram amounts. Compared to other luminol based systems, SignalFire™ ECL Reagent boasts a more robust signal and extended duration of signal output. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained with longer exposure.

20X LumiGLO® Reagent and 20X Peroxide 5ml each

价格:¥

货号:7003P
品牌:CST

经销:基因有限公司

SignalFire™ Plus ECL Reagent 20ml

价格:¥

货号:12630P
品牌:CST

经销:基因有限公司

20ml。一种极灵敏的化学发光底物,可通过western blot检测低皮克级别的蛋白质。发光时间延长,可持续数小时,允许多次曝光,兼容胶片和数码成像系统。极其强烈的信号输出,可检测低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体相较于传统ECL试剂要少6倍。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。
A highly sensitive chemiluminescent substrate capable of detecting low picogram amounts of protein by western blot analysis. SignalFire™ Plus ECL Reagent has an extended duration of signal output lasting several hours following blot exposure, allowing for multiple exposures with either film or a digital imaging system. The strong signal output allows detection of low abundance proteins, conservation of reagents, and short exposure times. 20ml.
SignalFire™ Plus ECL Reagent requires approximately five-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7077 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio.

SignalFire™ Elite ECL Reagent 20ml

价格:¥

货号:12757P
品牌:CST

经销:基因有限公司

20ml。一种超级灵敏的化学发光底物,可通过western blot检测低飞克级别的蛋白质。兼容胶片和数码成像系统。极其强烈的信号输出,可检测极低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体仅为传统ECL试剂的10分之一。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。
An ultra sensitive chemiluminescent substrate capable of detecting femtogram amounts of protein by western blot analysis. SignalFire™ Elite ECL Reagent is compatible with both film and digital imaging systems. The extremely intense signal output allows detection of very low abundance proteins, conservation of reagents, and short exposure times. 20ml.
SignalFire™ Elite ECL Reagent requires approximately ten-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio. 20 ml

20X LumiGLO® Reagent and 20X Peroxide 25ml each

价格:¥

货号:7003S
品牌:CST

经销:基因有限公司

各25ml。化学发光底物,遇HRP后发光,可以持续0.5-1小时,压片时间通常数秒到数分钟,允许重复压片或者调整压片时间。灵敏度高,可检测亚皮克级的蛋白质。
LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin

SignalFire™ ECL Reagent 500ml

价格:¥

货号:6883S
品牌:CST

经销:基因有限公司

增强的ECL化学发光检测试剂,500ml。具有极高的敏感性,可以检测低达皮克级水平的蛋白。与其他的发光系统相比较, SignalFire™ ECL 试剂可以产生更强的信号,可以更长时间的输出信号。底物暴露于HRP后,光发射立即达到最大并且可以持续0.5-2小时。光可以被记录在胶片上,一般暴露几秒钟。延长暴露时间可以增加灵敏度。
500 ml(250 ml each substrate). An enhanced luminol based system designed for use with CST™ HRP-linked secondary antibodies and Phototope®-HRP detection assays. SignalFire™ ECL Reagent is extremely sensitive and capable of detecting protein levels at low picogram amounts. Compared to other luminol based systems, SignalFire™ ECL Reagent boasts a more robust signal and extended duration of signal output. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained with longer exposure.

Phototope-HRP Western Blot Detection System, 50assays

价格:¥

货号:7072S
品牌:CST

经销:基因有限公司

50次反应。试剂盒含发光底物,HRP偶联的抗小鼠二抗和生物素标记蛋白分子量标记,灵敏度高,发光持续时间长,可检测亚皮克级的蛋白质。为标准的western blotting 应用中检测化学发光的蛋白而设计。蛋白和生物素化的分子量标记(已提供)可以在SDS-PAGE中分离,再被转移到膜上。与抗血清的一抗和辣根过氧化物酶(HRP) 标记的二抗孵育后,HRP标记的抗生物素抗体被结合,然后与LumiGLO ® 试剂反应,发出的光随后被捕获到X胶片上。
Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays.
20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
Anti-mouse IgG, HRP-linked Antibody #7076 250 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 500 µl
LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin

Phototope-HRP Western Blot Detection System, 50assays

价格:¥

货号:7071S
品牌:CST

经销:基因有限公司

50次反应。试剂盒含发光底物,HRP偶联的抗兔二抗和生物素标记蛋白分子量标记,灵敏度高,发光持续时间长,可检测亚皮克级的蛋白质。为标准的western blotting 应用中检测化学发光的蛋白而设计。蛋白和生物素化的分子量标记(已提供)可以在SDS-PAGE中分离,再被转移到膜上。与抗血清的一抗和辣根过氧化物酶(HRP) 标记的二抗孵育后,HRP标记的抗生物素抗体被结合,然后与LumiGLO ® 试剂反应,发出的光随后被捕获到X胶片上。
Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays.
20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
Anti-rabbit IgG, HRP-linked Antibody #7074 250 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 500 µl
LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin

SignalFire™ Plus ECL Reagent 100ml

价格:¥

货号:12630S
品牌:CST

经销:基因有限公司

100ml。一种极灵敏的化学发光底物,可通过western blot检测低皮克级别的蛋白质。发光时间延长,可持续数小时,允许多次曝光,兼容胶片和数码成像系统。极其强烈的信号输出,可检测低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体相较于传统ECL试剂要少5倍。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。
A highly sensitive chemiluminescent substrate capable of detecting low picogram amounts of protein by western blot analysis. SignalFire™ Plus ECL Reagent has an extended duration of signal output lasting several hours following blot exposure, allowing for multiple exposures with either film or a digital imaging system. The strong signal output allows detection of low abundance proteins, conservation of reagents, and short exposure times. 100ml.
SignalFire™ Plus ECL Reagent requires approximately five-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio.

SignalFire™ Elite ECL Reagent 100ml

价格:¥

货号:12757S
品牌:CST

经销:基因有限公司

100ml。一种超级灵敏的化学发光底物,可通过western blot检测低飞克级别的蛋白质。兼容胶片和数码成像系统。极其强烈的信号输出,可检测极低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体仅为传统ECL试剂的10分之一。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。
An ultra sensitive chemiluminescent substrate capable of detecting femtogram amounts of protein by western blot analysis. SignalFire™ Elite ECL Reagent is compatible with both film and digital imaging systems. The extremely intense signal output allows detection of very low abundance proteins, conservation of reagents, and short exposure times. 100ml.
SignalFire™ Elite ECL Reagent requires approximately ten-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio. 21 ml

Western Lightning Plus ECL化学发光HRP底物:Western Lightning Plus ECL 15ml X 2

价格:¥

货号:NEL103E001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Pro ECL化学发光HRP底物:Western Lightning Pro ECL 15ml X 2

价格:¥

货号:NEL120E001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning ECL Pro, 30 ml Western Lightning ECL Pro,非辐射光激发系统,用于检测膜蛋白,含有15ml发光增强试剂和15ml氧化试剂。

Western Lightning Ultra ECL化学发光HRP底物:Western Lightning Ultra ECL 10 ml x 2

价格:¥

货号:NEL111001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Ultra, 20 ml Western Lightning Ultra,一种超灵敏的HRP化学发光检测系统,检测量可低至mumg,光信号持久,可长达9小时之久。

Western Lightning Pro ECL化学发光HRP底物:Western Lightning Pro ECL 65ml X 2

价格:¥

货号:NEL120001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Plus ECL化学发光HRP底物:Western Lightning Plus ECL 65ml X 2

价格:¥

货号:NEL103001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning® Plus-ECL, Enhanced ChemiluminescenceSubstrate (130 ml) The Western Lightning™ Chemiluminescence Reagent Plus,非辐射光激发系统,用于检测膜蛋白,灵敏度高,可检测1-10pg蛋白,反应速度,反应信号持久。

Western Lightning Pro ECL化学发光HRP底物:Western Lightning Pro ECL 170ml X 2

价格:¥

货号:NEL121001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Plus ECL化学发光HRP底物:Western Lightning Plus ECL 170ml X 2

价格:¥

货号:NEL104001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning® Plus-ECL, Enhanced ChemiluminescenceSubstrate (340 ml) The Western Lightning™ Chemiluminescence Reagent Plus,非辐射光激发系统,用于检测膜蛋白,灵敏度高,可检测1-10pg蛋白,反应速度,反应信号持久。

Western Lightning Pro ECL化学发光HRP底物:Western Lightning Pro ECL 170ml X 4

价格:¥

货号:NEL122001EA
品牌:PerkinElmer

经销:基因有限公司

CDP-Star AP化学发光底物:Western Lightning(TM) CDP STAR 125ml

价格:¥

货号:NEL602001KT
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Ultra ECL化学发光HRP底物:Western Lightning Ultra ECL 55 ml x 2

价格:¥

货号:NEL112001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Ultra, 110 ml Western Lightning Ultra,一种超灵敏的HRP化学发光检测系统,检测量可低至mumg,光信号持久,可长达8小时之久。

CDP-Star AP底物加Nitro-Block-II增强剂:Western Lightning CDP-Star wit 100ml

价格:¥

货号:NEL616001KT
品牌:PerkinElmer

经销:基因有限公司

Western Lightning Plus ECL化学发光HRP底物:Western Lightning Plus ECL 170ml X 4

价格:¥

货号:NEL105001EA
品牌:PerkinElmer

经销:基因有限公司

Western Lightning® Plus-ECL, Enhanced ChemiluminescenceSubstrate (680 ml) The Western Lightning™ Chemiluminescence Reagent Plus,非辐射光激发系统,用于检测膜蛋白,灵敏度高,可检测1-10pg蛋白,反应速度,反应信号持久。

Western Lightning Ultra ECL化学发光HRP底物:Western Lightning Ultra ECL 110 ml x 2

价格:¥

货号:NEL113001EA
品牌:PerkinElmer

经销:基因有限公司

增强型ECL化学发光检测试剂盒(即用型) 60 ml (2 × A/B)

价格:¥

货号:E411-04
品牌:Vazyme

经销:南京诺唯赞生物

免疫印迹最后也是最重要一步就是酶标抗体与底物反应释放信号。本试剂盒以化学发光方法检测蛋白质或核酸类生物大分子。其原理是蛋白质或核酸在电泳后转移到印迹膜上,以辣根过氧化物酶HRP标记的抗体结合膜上的目的蛋白,或以HRP标记的探针直接或间接结合膜上的核酸。洗膜后用本试剂盒配制ECL工作液,在可见光下室温孵育数分钟,将印迹膜用保鲜膜包被固定于X光片曝光暗盒,然后转入暗室将胶片压在膜上曝光数秒至数小时。显影定影后蛋白质或核酸条带可清晰显示在X光片上。也可不进行胶片曝光,直接对印迹膜进行CCD扫描。

增强型ECL化学发光检测试剂盒(浓缩型) 5 ml (20 × A/B)

价格:¥

货号:E411-01
品牌:Vazyme

经销:南京诺唯赞生物

免疫印迹最后也是最重要一步就是酶标抗体与底物反应释放信号。本试剂盒以化学发光方法检测蛋白质或核酸类生物大分子。其原理是蛋白质或核酸在电泳后转移到印迹膜上,以辣根过氧化物酶HRP标记的抗体结合膜上的目的蛋白,或以HRP标记的探针直接或间接结合膜上的核酸。洗膜后用本试剂盒配制ECL工作液,在可见光下室温孵育数分钟,将印迹膜用保鲜膜包被固定于X光片曝光暗盒,然后转入暗室将胶片压在膜上曝光数秒至数小时。显影定影后蛋白质或核酸条带可清晰显示在X光片上。也可不进行胶片曝光,直接对印迹膜进行CCD扫描。

增强型ECL化学发光检测试剂盒(浓缩型) 10 ml (20 × A/B)

价格:¥

货号:E411-02
品牌:Vazyme

经销:南京诺唯赞生物

免疫印迹最后也是最重要一步就是酶标抗体与底物反应释放信号。本试剂盒以化学发光方法检测蛋白质或核酸类生物大分子。其原理是蛋白质或核酸在电泳后转移到印迹膜上,以辣根过氧化物酶HRP标记的抗体结合膜上的目的蛋白,或以HRP标记的探针直接或间接结合膜上的核酸。洗膜后用本试剂盒配制ECL工作液,在可见光下室温孵育数分钟,将印迹膜用保鲜膜包被固定于X光片曝光暗盒,然后转入暗室将胶片压在膜上曝光数秒至数小时。显影定影后蛋白质或核酸条带可清晰显示在X光片上。也可不进行胶片曝光,直接对印迹膜进行CCD扫描。

增强型ECL化学发光检测试剂盒(即用型) 250 ml (2 × A/B)

价格:¥

货号:E411-05
品牌:Vazyme

经销:南京诺唯赞生物

免疫印迹最后也是最重要一步就是酶标抗体与底物反应释放信号。本试剂盒以化学发光方法检测蛋白质或核酸类生物大分子。其原理是蛋白质或核酸在电泳后转移到印迹膜上,以辣根过氧化物酶HRP标记的抗体结合膜上的目的蛋白,或以HRP标记的探针直接或间接结合膜上的核酸。洗膜后用本试剂盒配制ECL工作液,在可见光下室温孵育数分钟,将印迹膜用保鲜膜包被固定于X光片曝光暗盒,然后转入暗室将胶片压在膜上曝光数秒至数小时。显影定影后蛋白质或核酸条带可清晰显示在X光片上。也可不进行胶片曝光,直接对印迹膜进行CCD扫描。

第1 / 1页 共28条 上一页 下一页

生物通 版权所有

未经书面授权,所有页面内容、图片和设计不得以任何形式进行复制

Copyright © 2000-2018 eBiotrade.com, All Rights Reserved

本栏目客服联系电话:广州 020-85524840转1060

本栏目广告联系电话:广州 020-87511980

主编信箱:

粤公网安备 44010602000434号 免责声明

22ms22ms128ms540ms1315ms