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产品名/货号/品牌 简介 包装  
DyLight 554 Phalloidin,300 units 1KIT

¥

货号:13054S
品牌:CST

经销:基因有限公司

DyLight™ 554偶联标记鬼笔环肽素,细胞骨架标记荧光染料,300unit
DyLight™ 554 Phalloidin,300 units

DyLight™350 Phalloidin, 300units 1KIT

¥

货号:12848S
品牌:CST

经销:基因有限公司

DyLight™350偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight™350 Phalloidin, 300units

DyLight™594 Phalloidin, 300units 1KIT

¥

货号:12877S
品牌:CST

经销:基因有限公司

DyLight™549偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight™594 Phalloidin, 300units

DyLight™650 Phalloidin, 300units 1KIT

¥

货号:12956S
品牌:CST

经销:基因有限公司

DyLight™650偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight™650 Phalloidin, 300units

DyLight™488 Phalloidin, 300units 1KIT

¥

货号:12935S
品牌:CST

经销:基因有限公司

DyLight™488偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight™488 Phalloidin, 300units

Alexa Fluor 488 Phalloidin, 300assays 1KIT

¥

货号:8878S
品牌:CST

经销:基因有限公司

Alexa Fluor® 488偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit,Excitation: 495, Emission: 518。 对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor® 488鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片随着ProLong® Gold Antifade Reagent #9071轻轻滑动,用合适的激发波长检测样品。
Alexa Fluor® 488 Phalloidin, 300assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 488 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.

Alexa Fluor(R) 555 Phalloidin, 300 assays 300 assays

¥

货号:8953S
品牌:CST

经销:基因有限公司

Alexa Fluor® 555标记的鬼笔环肽,细胞骨架标记荧光染料。一种自然出现的有毒的二环肽,存在于剧毒毒伞的子实体菌、鬼笔鹅膏中,对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor® 555鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片上滴加ProLong® Gold 抗淬灭试剂,用合适的激发波长检测样品。
300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 555 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold antifade reagent and examine specimen using appropriate excitation wavelength.

Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

Alexa Fluor(R) 647 Phalloidin, 300 assays 300 assays

¥

货号:8940S
品牌:CST

经销:基因有限公司

Alexa Fluor® 647标记的鬼笔环肽,细胞骨架标记荧光染料。一种自然出现的有毒的二环肽,存在于剧毒毒伞的子实体菌、鬼笔鹅膏中,对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor® 647鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片上滴加ProLong® Gold 抗淬灭试剂,用合适的激发波长检测样品。
300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 647 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold antifade reagent and examine specimen using appropriate excitation wavelength.

Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

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