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细胞生物学

活体成像试剂

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荧光素酶报告基因系统

细胞染料/标记示踪

蛋白质标记定位

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细胞因子/生长因子

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蛋白纯品对照

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细胞毒性

荧光原位杂交

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各类细胞(RNAlater保存/提取RNA)

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产品名/货号/品牌 简介 包装  
DAPI 1mg

价格:¥

货号:4083S
品牌:CST

经销:基因有限公司

1mg. DAPI是一种蓝色的荧光染料,靶向检测在DNA凹槽中的双链AT团(1)。染料上的一个分子与dsDNA上的每3个碱基对结合,达到大约20倍的荧光强度(2)。DAPI-DNA的荧光水平与DNA的含量相关。本产品溶于去离子水中的最大浓度为50mg/ml,溶于二甲基甲酰胺 中为澄明黄色液体。需要解热和(或)超生。对于标准荧光为基础的分析方法,DAPIstock 溶液需溶于磷酸盐缓冲液中或封片液中,终浓度为1到0.01ug/ml。
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content. This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.01 μg/mL. 1mg

Hoechst 33342 25mg

价格:¥

货号:4082S
品牌:CST

经销:基因有限公司

25 mg. Hoechst 33342 是一种荧光DNA染料,能够特异性地与双链富含AT的区域的凹槽结合。这些染料可以用于活的和已固定的细胞(1),正在复制或分裂的染色体不能使用Hoechst 33342染料。本产品溶于去离子水中的最大浓度为50mg/ml,溶于二甲基甲酰胺 中为澄明黄色液体。需要解热和(或)超生。对于标准荧光为基础的分析方法,Hoechst 33342 stock 溶液需溶于磷酸盐缓冲液中或封片液中,终浓度为1ug/ml。
Hoechst 33342 is a specific fluorescent DNA stain that bind within the minor groove of double-stranded AT-rich regions. These stains can be used on both live and fixed cells (1). Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye.This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.25 mg

Propidium Iodide (PI)/RNase Staining Solution 100ml

价格:¥

货号:4087S
品牌:CST

经销:基因有限公司

PI是一种荧光染料,可以插在基底和染色的DNA和RNA之间。特异的DNA染色时通过采用核糖核酸酶(RNase)清除RNA。PI/Rnase通常在荧光显微镜下染色细胞核,在流式细胞分析细胞周期时确定DNA的含量。处于细胞周期的G2和M期的细胞包含的DNA是G0和G1期细胞的2倍。S期的DNA含量处于两者之间。PI/Rnase染色试剂的激发和发射光最大分别在535nm和617nm(在光谱的桔红到红色的范围)。此染色试剂可以应与抗体串联,无约束力的干扰,适于多重检测中的DNA染色. 在荧光染色的最后一步,加入足够的未稀释的PI/Rnase染色试剂到浸没细胞或样本,室温孵育15分钟,分析前避光。100ml.
Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.

DRAQ5® 50µl

价格:¥

货号:4084S
品牌:CST

经销:基因有限公司

50ul. 一种能透过细胞膜的远红DNA荧光染料,可以应用在肝脏中或用来固定细胞。这种染料可以与GFP或FITC标记结合。 DRAQ5 ® 在流式细胞术和荧光显微镜中检测细胞内的DNA。流式细胞术:离心细胞,用按照1:5000 (1 µM)将 DRAQ5 ® 加到0.5ml的孵育缓冲液中。避光室温孵育5分钟,然后采用流式细胞术分析细胞。 免疫荧光:用PBS清洗细胞2次,每次5分钟。按照1:1000(5uM)用PBS稀释e DRAQ5 ®,避光室温孵育5分钟,用PBS清洗细胞一次,盖上带有褪色试剂涂层盖玻片,采用适当的激发光波长尽快检测细胞。
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications.
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. 
 Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

DRAQ7™ 1ml

价格:¥

货号:7406S
品牌:CST

经销:基因有限公司

DRAQ7™是一种远红荧光DNA染料,仅用来染色死亡和透化细胞的细胞核。它可以与GFP,FITC和PE的标记联合使用。准备细胞用于DRAQ7™染色,将细胞重悬于PBS中,细胞浓度不 不超过5×10 5细胞/毫升。每0.5毫升的细胞悬液,按照1:100加入5μl DRAQ7™l 终浓度达到3μM。轻轻地混匀,在冰上孵育10分钟。含有的DRAQ7™的细胞直接用于分析。
DRAQ7™ is a far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. It can be used in combination with GFP, FITC, and PE labels. Flow Cytometry: Prepare cells for DRAQ7™ staining by resuspending cells in PBS at a concentration of no more than 5 x 105 cells/ml. For each 0.5 ml of cell suspension, dilute DRAQ7™ 1:1000 by adding 5 μl to suspended cells, achieving a final concetration of 3 μM. Gently mix and incubate for 10 minutes on ice. Cells should be analyzed directly in the presence of DRAQ7.

DRAQ5® 200µl

价格:¥

货号:4084L
品牌:CST

经销:基因有限公司

200ul. 一种能透过细胞膜的远红DNA荧光染料,可以应用在肝脏中或用来固定细胞。这种染料可以与GFP或FITC标记结合。 DRAQ5 ® 在流式细胞术和荧光显微镜中检测细胞内的DNA。流式细胞术:离心细胞,用按照1:5000 (1 µM)将 DRAQ5 ® 加到0.5ml的孵育缓冲液中。避光室温孵育5分钟,然后采用流式细胞术分析细胞。 免疫荧光:用PBS清洗细胞2次,每次5分钟。按照1:1000(5uM)用PBS稀释e DRAQ5 ®,避光室温孵育5分钟,用PBS清洗细胞一次,盖上带有褪色试剂涂层盖玻片,采用适当的激发光波长尽快检测细胞。
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications.
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. 
 Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

DRAQ7

价格:¥

货号:7406P
品牌:CST

经销:基因有限公司

DRAQ7™是一种远红荧光DNA染料,仅用来染色死亡和透化细胞的细胞核。它可以与GFP,FITC和PE的标记联合使用。准备细胞用于DRAQ7™染色,将细胞重悬于PBS中,细胞浓度不 不超过5×10 5细胞/毫升。每0.5毫升的细胞悬液,按照1:100加入5μl DRAQ7™l 终浓度达到3μM。轻轻地混匀,在冰上孵育10分钟。含有的DRAQ7™的细胞直接用于分析。
DRAQ7™ is a far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. It can be used in combination with GFP, FITC, and PE labels. Flow Cytometry: Prepare cells for DRAQ7™ staining by resuspending cells in PBS at a concentration of no more than 5 x 105 cells/ml. For each 0.5 ml of cell suspension, dilute DRAQ7™ 1:1000 by adding 5 μl to suspended cells, achieving a final concetration of 3 μM. Gently mix and incubate for 10 minutes on ice. Cells should be analyzed directly in the presence of DRAQ7.

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