Annexin V-FITC Apoptosis Detection Kit was used:• in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.• for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.• for indirect measurement of flippase activity.
Annexin V-FITC Apoptosis Detection Kit was used:• in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.• for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.• for indirect measurement of flippase activity.
在正常细胞中,磷脂酰丝氨酸(PS) 只分布在细胞膜脂质双层的内侧,而在细胞凋亡早期,细胞膜中的磷脂酰丝氨酸(PS) 由脂膜内侧翻向外侧。Annexin V 是一种分子量为35-36 kDa 的Ca2+ 依赖性磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,因此Annexin V 被公认为检测细胞早期凋亡的灵敏指标之一。
将Annexin V 进行绿色荧光(FITC) 标记,以标记了的Annexin V 作为探针,利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶(Propidium Iodide, PI) 是一种核酸染料,它不能透过正常细胞或早期凋亡细胞完整的细胞膜,但对凋亡中晚期的细胞和坏死细胞,PI 能够透过细胞膜而使细胞核染红。因此将 Annexin V 与PI 匹配使用,就可以将处于不同凋亡时期的细胞区分开来。
在正常细胞中,磷脂酰丝氨酸(PS) 只分布在细胞膜脂质双层的内侧,而在细胞凋亡早期,细胞膜中的磷脂酰丝氨酸(PS) 由脂膜内侧翻向外侧。Annexin V 是一种分子量为35-36 kDa 的Ca2+ 依赖性磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,因此Annexin V 被公认为检测细胞早期凋亡的灵敏指标之一。
将Annexin V 进行绿色荧光(FITC) 标记,以标记了的Annexin V 作为探针,利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶(Propidium Iodide, PI) 是一种核酸染料,它不能透过正常细胞或早期凋亡细胞完整的细胞膜,但对凋亡中晚期的细胞和坏死细胞,PI 能够透过细胞膜而使细胞核染红。因此将 Annexin V 与PI 匹配使用,就可以将处于不同凋亡时期的细胞区分开来。
full length Annexin V protein corresponding to amino acids 1-319 of human origin conjugated to FITC fluorochrome; 50 µg5 ml 10x assay buffersufficient for 100 testsThe Annexin V Apoptosis Detection Kit utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis. Annexin V FITC binds to the membranes of apoptotic cells, displaying a characteristic staining pattern that may be viewed by fluorescence microscopy.non-conjugated control: sc-4252 BL
Annexin V-FITC Early Apoptosis Detection Kit使研究者能够从细胞群中鉴定出早期凋亡细胞。Annexin V-FITC结合蛋白能结合到细胞表面表达丝氨酸磷脂(一种早期凋亡标志)。细胞用碘化丙啶(PI,一种非细胞可透过性DNA染料)染色能指示出坏死的细胞。细胞同时用PI和annexin V-FITC染色能指示出较晚阶段的凋亡和早期坏死。本试剂盒提供的试剂足够进行100次分析,250 μl/次。 The Annexin V-FITC Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-FITC conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with propidium iodide (PI), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both PI and annexin V-FITC demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.