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产品名/货号/品牌 简介 包装  
Resazurin Cell Viability Kit, 25ml each 1KIT

目录价:¥

货号:11884S
品牌:CST

经销:基因有限公司

反应种属:All

刃天青钠盐细胞活性检测试剂盒,25ml. 刃天青(Resazurin)细胞活性试剂盒是荧光检测法,检测细胞的代谢活性。通过活细胞代谢中的脱氢酶,蓝色无荧光刃天青试剂被还原为高度荧光类试卤灵。这种转换只发生在活细胞中,因此,试卤灵的产生量与样品中的活细胞数成正比。检测中形成的试卤灵,可以用荧光光度计(Ex=530-570 nm, Em=590-620 nm)的相对荧光单位(RFU)来测量并量化。刃天青(Resazurin)细胞活性试剂盒能够检测活细胞中代谢酶转换刃天青产生的试卤灵。该试剂盒预计能在大多数细胞株中使用。对于大多数实验中,0.02-2×105个细胞/孔应该是足够的,但也取决于细胞类型和孵育时间。为获得最佳结果,推荐进行细胞数滴定和孵育时间进程分析(图1中所示)。注:微生物污染物将减少刃天青转化为试卤灵,从而产生假阳性结果。自体荧光化合物可能会对这种测定方法有干扰。
Resazurin Cell Viability Kit, 25ml each( 2500 assays (96 well format) )The Resazurin Cell Viability Kit detects resorufin produced from resazurin conversion by metabolic enzymes in live cells. This kit is expected to work in most cell lines. For most experiments, 0.02-2x105 cells/well should be sufficient, but this can vary depending on the cell type and incubation time. For best results, a cell number titration and incubation time course (as shown in Figure 1) is recommended. 
 Note: Microbial contaminants will reduce resazurin to resorufin, yielding false positive results. Autofluorescent compounds may interfere with this assay.
The Resazurin Cell Viability Kit is a fluorescent assay that detects cellular metabolic activity. The blue nonfluorescent resazurin reagent is reduced to highly fluorescent resorufin by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of resorufin produced is proportional to the number of viable cells in the sample. The resorufin formed in the assay can be quantified by measuring the relative fluorescence units (RFU) using a fluorometer (Ex=530-570 nm, Em=590-620 nm).

Senescence beta-Galactosidase Staining Kit, 1 Kit 1 Kit

目录价:¥

货号:9860S
品牌:CST

经销:基因有限公司

该试剂盒检测培养细胞中pH为6时β-galactosidase 的活性。pH 6时β-galactosidase 的活性仅见于衰老细胞,未见于衰老前细胞、静止细胞或永生化细胞。Senescence β-Galactosidase Staining Kit设计为pH 6时检测β-galactosidase 活性,此活性为衰老细胞独有,未见于非衰老细胞、静止细胞或永生化细胞。该试剂盒包括该检测所需所有试剂。
The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.



XTT Cell Viability Kit, 1 Kit 1 Kit

目录价:¥

货号:9095S
品牌:CST

经销:基因有限公司

XTT细胞活力分析试剂盒是一种比色分析方法,可以检测细胞的代谢能力。在分析方法中,黄色四氮唑在代谢活跃的细胞中可以变成高度着色的甲臜染料。这种转变只存在于活力旺盛的细胞中,因此甲臜染料的产生量与样本中活力旺盛的细胞成正比。在此方法中形成的甲臜染料溶于水,可以用分光光度计在波长为450nm处测其吸光值进行定量。电子偶联剂如PMS( N-甲基吩嗪 甲基硫酸盐)可以显著提高XTT的降低效率。XTT细胞活力分析试剂盒可以检测到在细胞中线粒体酶作用下由XTT转变成的甲臜染料。 因为线粒体酶在细胞死亡后失去活性,橘红色的甲臜染料只存在活力旺盛的细胞中。 XTT细胞活力分析试剂盒有望在大部分细胞系中应用。细胞类型和孵育时间的不同, 0.2-2x10 4 cells/well对于大多数实验室足够的。为了取得最理想的结果,建议做细胞浓度梯度
细胞活力和增殖分析广泛应用于药物的研发,以进一步研究生长因子、细胞因子和细胞毒制剂。在早期药物发现的复合筛选和后期药品的安全性和毒性研究中,高通量筛查需要可靠、敏感和简单的分析方法,以具备分析大量样本的能力。采用四氮唑如MTT、XTT、WST-1等比色法分析细胞活力的方法是在活细胞中四氮唑变成高度着色的甲臜染料复合物基础上发展的(1、2)。相比之下,细胞增殖分析方法如活细胞中用放射性碘和BrdU标记的DNA后,用合并碘(对样本的放射性定量)或BrdU(用anti-BrdU 抗体)来定量,而XTT分析方法不需要放射性物质、固定细胞或细胞透化。因此,与上述细胞分析方法不同,用XTT分析过的细胞还可以用于进一步分析。
1000 assays (96 well format). The XTT Cell Viability Assay Kit is a colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and can be quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulfate), can significantly improve the efficiency of XTT reduction in cells.

The XTT Cell Viability Kit detects formazan dye produced from XTT conversion by mitochondrial enzymes in cells. Because these mitochondrial enzymes are inactivated shortly after cell death, the orange colored formazan dye only appears in viable cells. This XTT Cell Viability Kit is expected to work in most cells lines. Variable with cell type and incubation time employed in the assay, 0.2-2x104 cells/well should be sufficient for most experiments. For the best result, a cell number titration (as shown in Figure 1) is recommended.

Cell viability and proliferation assays are widely used in drug discovery for the study of growth factors, cytokines, and cytotoxic agents. High throughput screening, in early drug discovery compound screening and in later drug safety and toxicity studies, requires a reliable, sensitive, and simple assay with the ability to analyze a large number of samples. Colorimetric cell viability assays using tetrazolium salt, such as MTT, XTT, WST-1, etc. were developed based on live cells reduction of tetrazolium salt into highly colored formazan compounds (1,2). In contrast to cell proliferation assays, such as radioactive thymidine or BrdU labeling of DNA in live cells followed by quantification of the incorporated thymidine (by quantifying sample radioactivity) or BrdU (using anti-BrdU antibody), the XTT assay doesn’t require radioactive materials, cell fixing, or cell permeabilization. Thus, unlike alternative cellular analysis assays, cells examined in the XTT assay may be used for further analysis.

BrdU Cell Proliferation Assay Kit, 1 Kit 1 Kit

目录价:¥

货号:6813S
品牌:CST

经销:基因有限公司

BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU,使用的抗体为抗BrDU抗体。当细胞培养于含BrDU的标记培养基中,该胸腺嘧啶类似物可代替胸腺嘧啶掺入到增殖细胞的新合成DNA中。吸除标记培养基后,用固定/变性液固定细胞同时使DNA变性。然后加入BrdU鼠源单抗检测掺入的BrdU(DNA变性使得掺入的BrdU易于为抗体所识别)。Anti-mouse IgG, HRP-linked antibody用来识别联胞检测抗体。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和掺入细胞的BrdU的量成正比, BrdU是细胞增殖的直接证明。BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU。BrDU标记的DNA变性后能够为试剂盒中的BrdU Mouse mAb检测到。BrdU Mouse mAb不与内源性DNA发生交叉反应。依细胞种类和孵育时间不同 ,每孔0.2-2x104个细胞即足够大部分实验。为了得到最佳实验结果,我们推荐进行细胞数滴度实验
500 assays (96 well format). The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Then a BrdU mouse mAb is added to detect the incorporated BrdU (The denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups.

BrdU Cell Proliferation Chemiluminescent Assay Kit, 1 Kit 1 Kit

目录价:¥

货号:5492S
品牌:CST

经销:基因有限公司

BrdU细胞增殖化学发光检测试剂盒使用Anti-BrdU抗体检测细胞增殖时掺入DNA中的5-bromo-2’-deoxyuridine (BrdU)。使用含有BrdU的培养基培养细胞,这种嘧啶类似物能够在新DNA和成时取代胸腺嘧啶。移除培养基后,使用我们的固定/变性液以固定细胞,变性DNA。DNA变性能提高BrdU的掺入以便被抗体检测到。然后加入BrdU鼠单抗以检测掺入的BrdU。Anti-mouse IgG,HRP-偶联的抗体用以结合抗体。然后加入化学发光试剂。发射光的强度以相对光单位测得(RLU),与掺入细胞的BrdU量成正比,是细胞增殖的直接指示。BrdU细胞增殖化学发光检测试剂盒能够检测在细胞增殖时掺入细胞DNA的BrdU。BrdU标记的DNA必须经过变性才能使用试剂盒中的BrdU Mouse mAb检测。BrdU Mouse mAb与内源性DNA没有交叉反应。0.2-2x104 cells/well足以应对大多数实验类型,细胞数目取决于细胞类型和孵育时间。为了得到最好的结果,建议对细胞进行梯度稀释
500 assays (96 well format)。 The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody. A BrdU mouse mAb is then added to detect the incorporated BrdU. Anti-mouse IgG, HRP-linked Antibody is used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Chemiluminescent Assay Kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA must be denatured to be detected by the BrdU mouse mAb used in this kit. This BrdU mouse mAb does not cross-react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups. For best results, a cell number titration (Figure 1) is recommended.

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