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分类简介:    高质量的化学激活剂和抑制剂与激活状态和总蛋白抗体配合使用,对于探测关键信号节点和通路非常有用。
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产品名/货号/品牌 简介 包装  
Sodium Fluoride (Fluoride), 1.0 ml 1.0 ml

价格:¥

货号:P0759S
品牌:NEB

经销:基因有限公司

Sodium Orthovanadate (Vanadate), 1.0 ml 1.0 ml

价格:¥

货号:P0758S
品牌:NEB

经销:基因有限公司

Sodium Fluoride (Fluoride), 5.0 ml 5.0 ml

价格:¥

货号:P0759L
品牌:NEB

经销:基因有限公司

Sodium Orthovanadate (Vanadate), 5.0 ml 5.0 ml

价格:¥

货号:P0758L
品牌:NEB

经销:基因有限公司

Everolimus, 1mg 1KIT

价格:¥

货号:12017S
品牌:CST

经销:基因有限公司

依维莫司,亦称作RAD001,与FKBP12结合形成复合体,结合并抑制mTORC1活性。
Everolimus, 1mg

Paclitaxel, 1 mg 1 mg

价格:¥

货号:9807S
品牌:CST

经销:基因有限公司

紫杉醇属于抗肿瘤和抗白血病药物紫杉烷家族(3)。通过结合β-微管蛋白和促进微管装配,紫杉醇可以防止微管解聚,并阻止正常细胞分裂(1-3)。紫杉醇引起的微管功能障碍能够导致异常的细胞有丝分裂和偶发的凋亡。紫杉醇诱导有丝分裂阻遏的IC50是4nM (4)。Paclitaxel为1mg粉末。-20ºC或更低温度保存。使用前用1.15 ml DMSO稀释溶解至1 mM贮存液。工作浓度为100 nM-1000 nM,将稀释DMSO贮存液1:10,000 到 1:1000。用需要的浓度处理细胞6-48小时。Pacli
1 mg. Paclitaxel belongs to the taxane family of antitumor and antileukemic agents (3). By binding to β-tubulin and promoting the assembly of microtubules, paclitaxel prevents microtubual depolymerization and blocks normal cell division (1-3). The microtubule dysfunction induced by paclitaxel results in aberrant cell mitosis and sometimes apoptosis. The IC50 of paclitaxel-induced mitotic block is 4 nM (4).
Paclitaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.15 ml DMSO to make a 1 mM paclitaxel stock solution. For working concentrations of 100 nM-1000 nM, dilute DMSO stock 1:10,000 to 1:1000. Treat cells with the desired concentration for 6-48 hours. Paclitaxel is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Tyrphostin AG 1478, 400 ug 400 ug

价格:¥

货号:9842S
品牌:CST

经销:基因有限公司

酪氨酸磷酸化抑制剂AG1478是一种特别选择性抑制EGFR (ErbB1)的酪氨酸激酶抑制剂,体外实验中,IC50值为3 nM(1,2)。用50-150 nM酪氨酸磷酸化抑制剂AG1478处理细胞可以充分阻断EGFR在体内的激活(3)。除了EGFR, AG 1478还能够抑制癌细胞因辐射引起的ErbB4激活(4)。AG 1478无论是单独使用,还是联合其他处理方式,在评价其抗肿瘤和抗纤维化的效果中都表现出不错的应用前景。酪氨酸磷酸化抑制剂AG1478以400μg干粉形式供应。存储于-20ºC或更低的温度下
400 µg. Tyrphostin AG 1478 is a tyrosine kinase inhibitor specifically selective to EGFR (ErbB1), with an IC50 of about 3 nM in vitro (1,2). Treatment of cell with 50-150 nM of AG 1478 can substantially block EGFR activiation in vivo (3). In addition to EGFR, AG 1478 also inhibits ErbB4 activation induced by radiation in cancer cells (4). Testing of AG 1478 alone or in combination with other treatments to assess anti-tumor and anti-fibrotic effectiveness has yielded promising results (5-8).
Tyrphostin AG 1478 is supplied as 400 μg powder. Store at or below -20ºC. Before use, dissolve powder in 1.23 ml DMSO to make a 1 mM 
 Tyrphostin AG 1478 stock solution. For working concentrations of 100 nM-200 nM, dilute DMSO stock 1:10,000 to 1:5,000. Treat cells with the desired concentration for 30 minutes. Tyrphostin AG1478 is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Docetaxel, 1 mg 1 mg

价格:¥

货号:9886S
品牌:CST

经销:基因有限公司

多烯紫杉醇属于抗肿瘤和抗白血病药物的紫杉醇家族(1)。作为一种微管稳定药物,多烯紫杉醇能够充当需要微管动力学如细胞分裂的抑制剂,现在用于乳腺癌、前列腺癌和肺癌的治疗(2)。Docetaxel为1mg粉末。-20ºC或更低温度保存。使用前用1ml DMSO稀释溶解至1mg/ml贮存液。推荐工作浓度为组织培养液中含100ng/ml。处理细胞4-24小时,贮存于-20ºC。
1 mg. Docetaxel belongs to the taxane family of antitumor and antileukemic agents (1). A microtubule-stabilizing agent, docetaxel acts as an inhibitor of cellular functions that require microtubule dynamics including cell division, and is now in use as a treatment for breast, prostate and lung cancer (2).
Docetaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1 ml DMSO to make a 1 mg/ml stock solution. The suggested working concentration is 100 ng/ml in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Roscovitine, 1 mg 1 mg

价格:¥

货号:9885S
品牌:CST

经销:基因有限公司

核抑制剂是一种能透过细胞的周期蛋白依赖性激酶CDK1 (cdc2),CDK2和CDK5的选择性抑制剂(1)。作为一种嘌呤类似物,该药物竞争性结合催化裂口的ATP位点。使用roscovitine处理培养的细胞能够引起细胞周期捕获或者凋亡(1-4)。该药对于cdc2活性的体外IC50是0.65 μM . Roscovitine [(R) stereoisomer] 为1mg粉末。-20ºC或更低温度保存。使用前用143 μl DMSO或MeOH稀释溶解至20mM贮存液。推荐工作浓度为组织培养液中20 μM 。
1 mg. Roscovitine is a cell permeable reversible selective inhibitor of cyclin-dependent kinases CDK1 (cdc2), CDK2 and CDK5 (1). A purine analog, this drug competes for the binding site of ATP in the catalytic cleft. Treatment of cultured cells with roscovitine can cause cell cycle arrest or apoptosis (1-4). The IC50 for cdc2 activity is 0.65 μM in vitro (1).
Roscovitine [(R) stereoisomer] is supplied as a 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 143 μl DMSO or MeOH to make a 20 mM stock solution. The suggested working concentration is 20 μM in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Vorinostat (SAHA) 伏立诺他

价格:¥

货号:12520S
品牌:CST

经销:基因有限公司

5 mg. MW=264.32 g/mol. C14H20N2O3. >99%. Vorinostat (SAHA) is a histone deacetylase (HDAC) inhibitor that acts by binding to and blocking the active site of the enzyme (1). Both class I and class II HDACs are inhibited by vorinostat at nanomolar concentrations (1-3). Research studies have shown that vorinostat causes the accumulation of acetylated histones, certain transcription factors, and other nonhistone proteins, which regulate gene expression and protein function. This leads to cell cycle arrest, differentiation, and/or apoptosis in many transformed cell types treated with micromolar concentrations of vorinostat. Vorinostat is supplied as a lyophilized powder. For a 20 mM stock, reconstitute the 5 mg in 945.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 2-24 hr. Soluble in DMSO at 66 mg/ml; soluble in ethanol at 2 mg/ml with slight warming; very poorly soluble in water with maximum solubility ~20-50 µM.

Dasatinib, 5 mg 5 mg

价格:¥

货号:9052S
品牌:CST

经销:基因有限公司

达沙替尼是一种Src和Bcr-Abl酪氨酸激酶的小分子抑制剂,针对这两种激酶的IC50值分别为0.55和3.0nm(1)。伊马替尼和达沙替尼有相似的分子机制,但是达沙替尼对伊马替尼耐药的细胞系有较强的作用,并且对血小板衍生生长因子(PDGF)有较强的抑制作用.提供的达沙替尼为冻干粉。对于5mM浓度贮存液,在5mg冻干粉中加入2.05 ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是50-500 nM,处理2-6小时。能溶于DMSO(200 mg/ml),难溶于乙醇和水,在水中的最大溶解度
>99% 5 mg.C22H26ClN7O2S. Dasatinib is a small molecule inhibitor of both the Src and Bcr-Abl tyrosine kinases, with IC50 values of 0.55 and 3.0 nM for the isolated kinases, respectively (1). Imatinib and dasatinib have similar inhibitory mechanisms, but dasatinib displays greater potency in imatinib-resistant cell lines and greater inhibitory effect on platelet derived growth factor (PDGF) (2,3).
Dasatinib is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 2.05 mL DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 50-500 nM for 2-6 hours. Soluble in DMSO at 200 mg/mL; very poorly soluble in ethanol and water with maximum solubility in water estimated to be about 10 μM.

Imatinib, 5 mg 5 mg

价格:¥

货号:9084S
品牌:CST

经销:基因有限公司

伊马替尼是一种酪氨酸激酶(TK)抑制剂,是Bcr-Abl,PDGF受体和c-Kit TKs的特异性ATP结合位点拮抗物(1-3)。伊马替尼在慢性髓细胞样白血病(CML)的临床试验中结果很不错,已经成为有针对性的癌症治疗的范例(4-6)。利用磷酸化酪氨酸通路的信号传导已经被广泛研究,酪氨酸磷酸化与许多细胞生长和分化途径有关(7-9)。因为观察到的CML的状态依赖于完整的Bcr-Abl酪氨酸激酶活性,我们做了多方面的工作来确认Bcr-Abl的底物和引起髓系分化的可能机制。许多研究小组已经发现了CML胚细胞和B
>99% 5 mg. C29H31N7O + CH4SO3. Imatinib is a tyrosine kinase (TK) inhibitor that is a relatively specific ATP-binding site antagonist of Bcr-Abl, PDGF receptor, and c-Kit TKs (1-3). Results are encouraging in chronic myeloid leukemia (CML) clinical trials and imatinib has become a paradigm for targeted cancer therapeutics (4-6). Signal transduction through phospho-tyrosine pathways has been studied extensively, and tyrosine phosphorylation has been linked to multiple cell growth and differentiation pathways (7-9). Because the observed leukemic state of CML is dependent on the intact Bcr-Abl tyrosine kinase activity, extensive work has been done to identify substrates of Bcr-Abl and thus possible mechanisms leading to a myeloid expansion. Many groups have characterized prominent tyrosine-phosphorylated protein substrates in both CML blasts and Bcr-Abl-expressing cell lines, including SHIP, c-Cbl, Dok, Shc, and CrkL (10-15). In addition, key signal transduction pathways involving PI3 kinase, Ras, Myc, and Stat5 are also activated in a Bcr-Abl kinase-dependent manner (16).
Imatinib is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 847.9 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 μM for 1-2 hours. Soluble in DMSO at 100 mg/ml; poorly soluble in ethanol. Soluble in water at 200 mg/ml.

LY294002 (PI3 Kinase Inhibitor) 1.5 mg

价格:¥

货号:9901S
品牌:CST

经销:基因有限公司

LY294002在体内是一个高度特异性的PI3K激酶抑制剂。在50 uM 浓度下,它可以特异地抑制 PI3K激酶的活性(IC50=0.43 μg/ml; 1.40 μM),但是不抑制其他的脂质和蛋白激酶活性,比如PI4 kinase, PKC, MAP kinase 或 c-Src (1)。 LY294002可溶解在DMSO或乙醇中。体外实验或着用于细胞实验时,可以将其稀释于缓冲液中以得到所需的浓度。用于培养的细胞时,CST建议您用LY294002预处理1h,直到刺激结束。研究表明LY294002可以抑制
1.5 mg. LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50 μM, it specifically abolished PI3 kinase activity (IC50=0.43 μg/ml; 1.40 μM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 is soluble in DMSO or ethanol. For use with in vitro or cell-based assays, it may be diluted into aqueous buffers to yield the desired concentrations. For experiments with cultured cells, CST recommends treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.
For 10 mM stock, reconstitute 1.5 mg in 488 µl DMSO. For 50 mM stock, reconstitute 1.5 mg in 98 µl DMSO. Store aliquots at -20ºC.

PD98059 (MEK1 Inhibitor)1.5 mg 1.5 mg

价格:¥

货号:9900S
品牌:CST

经销:基因有限公司

PD98059 (MEK1 抑制子)是MEK1 活化和MAP激酶级联反应的高选择性抑制子(1-4)。PD98059结合MEK1的非活化形式,并阻止上游活化因子如c-Raf的活化作用 (3)。本产品为冻干的黄色粉末。配置20 mM储存品时,取1.5 mg加入到280 µl DMSO 中(5 mg溶于933.3 μl体积中),配置50 mM储存品时,取1.5 mg加入到112 µl DMSO 中(5 mg溶于373.3 μl体积中).分装并储存于-20ºC,详细信息请查看附录中的MSDS。该产品只用于体内研究
1.5 mg(s) or 5 mg(L). PD98059 (MEK1 Inhibitor) has been shown to act in vivo as a highly selective inhibitor of MEK1 activation and the MAP kinase cascade (1-4). PD98059 binds to the inactive forms of MEK1 and prevents activation by upstream activators such as c-Raf (3).
Supplied as a lyophilized yellow powder. For 20 mM stock, reconstitute 1.5 mg in 280 µl DMSO (5 mg in 933.3 μl). For 50 mM stock, reconstitute 1.5 mg in 112 µl DMSO (5 mg in 373.3 μl). Store in aliquots at -20ºC. See enclosed MSDS for further information. This product is for in vitro research use only and is not intended for use in humans or animals.

Rapamycin, 10 nmol 10 nmol

价格:¥

货号:9904S
品牌:CST

经销:基因有限公司

Rapamycin(雷帕霉素)是一种具有抗真菌和免疫抑制活性的细菌大环内酯(1)。Rapamycin与亲免素FKBP12形成复合物,从而抑制FRAP/ mTOR的活性(酵母中的TOR)(2,3)。雷帕霉素处理细胞后,导致p70 S6激酶去磷酸化并失活。雷帕霉素也导致4E-BP1/PHAS1去磷酸化,从而促进其与eIF4E结合并抑制其活性(4,5)。这个特性已被证明是雷帕霉素阻止蛋白质合成,及使细胞周期停滞在G1期的基础(6,7)。然而,也有人认为雷帕霉素抑制G1 / S期转换可能是由于其对cyclin D
10 nmol. Rapamycin is a bacterial macrolide with antifungal and immunosuppressant activities (1). Rapamycin forms a complex with the immunophilin FKBP12 which then inhibits the activity of FRAP/ mTOR (TOR in yeast) (2,3). Rapamycin treatment of cells leads to the dephosphorylation and inactivation of p70 S6 kinase. Rapamycin also leads to the dephosphorylation of 4E-BP1/PHAS1, thereby promoting its binding to and inactivation of eIF4E (4,5). This activity has been shown to be the basis for Rapamycin's ability to block protein synthesis and to arrest cell cycle progression in the G1-phase (6,7). However, it has been suggested that Rapamycin's inhibition of the G1/S transition may be the consequence of its effect on cyclin D1 mRNA and protein stability (8).
Rapamycin is supplied as a lyophilized powder. For a 100 µM stock, resuspend 10 nmol (9.1 µg) in 100 µl methanol or DMSO. Rapamycin is soluble in DMSO and methanol. For experiments with cultured cells, CST recommends pretreating with 10 nmol of this inhibitor for one hour prior to stimulation.

MG-132, 1mg 1KIT

价格:¥

货号:2194S
品牌:CST

经销:基因有限公司

反应种属:Human

蛋白酶体和钙蛋白酶抑制剂
MG-132, 1mg

Bortezomib, 2.5 mg 2.5 mg

价格:¥

货号:2204S
品牌:CST

经销:基因有限公司

硼替佐米(C19H25BN4O4)——硼酸二肽,是一种特异的、有效的、可逆的蛋白酶抑制剂,对抑制细胞存活和细胞周期进程的蛋白质有一个稳定效应,如p53(1,2)。研究者发现硼替佐米能抑制NF-κB的活化和核转运,从而减少早期肿瘤存活(3)。能干扰细胞存活和其他重要通路的、硼替佐米稳定的、错误折叠的、泛素化的蛋白的增加能进一步加强它的抗肿瘤作用. 提供的硼替佐米为冻干粉。对于1mM浓度贮存液,在2.5mg冻干粉中加入6.51ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是1-1000nM
>99% 2.5 mg.C19H25BN4O4. Bortezomib (C19H25BN4O4), a boronic acid dipeptide, is a specific, potent, and reversable proteasome inhibitor that has also been shown to have a stabilizing effect on proteins that inhibit cell survival and cell cycle progression, such as p53 (1,2). Researchers have demostrated that bortezomib inhibits activation and nuclear translocation of NF-κB, subsequently decreasing early tumor survival (3). The increase in bortezomib-stablilized, misfolded, and ubiquitinated proteins that interfere with cell survival and other important pathways further support its anti-tumor effects
Bortezomib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 6.51 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-1000 nM for 2-48 hours. Soluble in DMSO and ethanol at 200 mg/ml, very poorly soluble in plain water.

Crizotinib, 2.5 mg 2.5 mg

价格:¥

货号:4401S
品牌:CST

经销:基因有限公司

克唑替尼也被称为PF-02341066,是一种新型的ATP竞争性受体激酶抑制剂,对c-Met和间变性淋巴瘤激酶(ALK)等超过120多样激酶显示出较高的特异性(1,2)。研究人员证明,克唑替尼抑制c-Met的磷酸化和c-Met依赖性增殖,体外人体肿瘤细胞的迁移和侵袭(IC50值分别为5-20nM)(1)。克唑替尼对抗组成性激活的致癌融合蛋白核磷蛋白(NPM)-ALK,抑制其磷酸化(平均IC50为24 nM),抑制细胞生长,在ALK-阳性ALCL (间变性大细胞淋巴瘤) 细胞系KARPAS-299和SU-D
>99% 2.5 mg. C21H22Cl2FN5O. Crizotinib, also known as PF-02341066, is a novel, ATP-competitive receptor kinase inhibitor, showing high specificity for c-Met and anaplastic lymphoma kinase (ALK) over 120 other diverse kinases (1,2). Researchers have shown that crizotinib inhibits c-Met phosphorylation and c-Met-dependent proliferation, migration, and invasion of human tumor cells in vitro (IC50 values of 5–20 nM) (1). Crizotinib is effective against the constitutively active oncogenic fusion protein nucleophosmin (NPM)-ALK, inhibiting its phosphorylation (mean IC50 of 24 nM), inhibiting cell growth, and inducing G1-S phase cell cycle arrest and apoptosis in the ALK-positive ALCL (anaplastic large-cell lymphoma) cell lines KARPAS-299 and SU-DHL-1
Crizotinib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 5.55 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µM for 2-6 h. Soluble in DMSO at 25 mg/ml with warming; very poorly soluble in water with maximum solubility ~10-20 µM.

Doxorubicin, 5 mg 5 mg

价格:¥

货号:5927S
品牌:CST

经销:基因有限公司

多柔比星,一种蒽环类抗生素,能抑制哺乳动物中DNA和RNA的合成,也是一种非常有效的抗肿瘤药物(1,2)。多柔比星通过嵌入DNA双螺旋和稳定拓扑异构酶II裂解复合物来结合到核酸,导致DNA在特异的多柔比星诱导的位点发生丝条断裂(3)。在人乳腺癌细胞(MCF7)中,多柔比星能通过一种剂量依赖的方式抑制DNA合成,与生长抑制相符合(4)。研究者还发现多柔比星能够有效抑制人DNA拓扑异构酶I(5)。提供的多柔比星为冻干粉。对于10 mM浓度贮存液,在5mg冻干粉中加入860 µl DMSO。工作浓度和处理时间根
>99% 5 mg. C27H29NO11 • HCl . Doxorubicin, an anthracycline antibiotic, inhibits DNA and RNA synthesis in mammalian cells and has been shown to be a very effective anti-tumor agent (1,2). Doxorubicin binds to nucleic acids by intercalating the DNA double helix and stabilizing topoisomerase II cleavage complexes, leading to DNA strand breaks at specific doxorubicin-induced sites (3). Doxorubin has been shown to inhibit DNA synthesis in a dose-dependent manner in MCF7 cells, which corresponds closely with growth inhibition (4). Researchers have also demonstrated that doxorubicin effectively inhibits human DNA topoisomerase I (5).
Doxorubicin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 860 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-5 µM for 12-24 hours. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol; soluble in water at 10 mg/ml with slight warming.

Bisindolylmaleimide I, Hydrochloride, 500 ug 500 ug

价格:¥

货号:9841S
品牌:CST

经销:基因有限公司

Bisindolylmaleimide I(BIS)是PKC的强力抑制剂(1,2)。BIS在体外试验的IC50,对PKCα/β/γ是10-20nM,对PKCδ/ε是100-200nM。在体外对PKCζ的IC50是6uM,表明BIS是此亚型非常弱的抑制剂。体内细胞分析BIS对PKC的IC50在0.2-2uM(1,3)。Bisindolylmaleimide I 以500ug粉剂提供。储存在-20℃。使用前,将粉末溶解于0.28ml DMSO中产生4mM原液。对于工作浓度为2uM-4uM,将原液按1:2000
500 µg. Bisindolylmaleimide I (BIS) is a potent inhibitor of PKC (1,2). In vitro the IC50 of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC50 for PKCζ is about 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays the IC50 of BIS for PKC is between 0.2-2 μM (1,3).
Bisindolylmaleimide I is supplied as 500 μg powder. Store at or below -20ºC. Before use, dissolve powder in 0.28 ml DMSO to make a 4 mM bisindolylmaleimide I stock solution. For working concentrations of 2 μM-4 μM, dilute DMSO stock 1:2000 to 1:1000. Treat cells with the desired concentration for 30 minutes. Bisindolylmaleimide I is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Calyculin A, 10 ug 10 ug

价格:¥

货号:9902S
品牌:CST

经销:基因有限公司

花萼海绵诱癌素A丝氨酸/苏氨酸抑制剂)与冈田软海绵酸相比较,是一种更加有潜力的磷酸酶抑制剂(2)。Western blot实验结果显示,用100nM花萼海绵诱癌素A处理细胞30分钟后,采用苏氨酸磷酸化多克隆抗体#9381可以检测到苏氨酸发生磷酸化。对抗PP1的抑制活性IC50值大约为2nM。对抗PP2A的抑制活性IC50值大约为0.5-1.0nM。冈田软海绵酸仅降低PP2A的活性而对PP1的活性几乎没有影响,与其不同的是花萼海绵诱癌素A可以抑制这两种磷酸酶(1)。花萼海绵诱癌素A和冈田软海绵酸都不能抑制酸
10 µg. Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM. Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).

Cyclosporin A, 100 mg 100 mg

价格:¥

货号:9973S
品牌:CST

经销:基因有限公司

本产品的包装规格是100mg粉末。-20ºC保存。Cyclosporin A粉末能够溶解在乙醇(或者DMSO)中而配制成母液,母液也需要在-20ºC保存。工作液的浓度和处理时长可根据不同的实验目的而变化,通常工作液的浓度约为100 nM。钙调磷酸酶是cyclosporin A (CsA) (3) 和 FK-506 (又叫他克莫司和藤霉素)两个免疫抑制剂的重要靶点,cyclosporin A (CsA)和 FK-506 能够抑制抗原和分裂素对T细胞的激活,这两个药物分别与亲免疫因子cyclophilin和F
100 mg。 Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models
Cyclosporin A is supplied as a 100 mg powder. Store at -20ºC. Cyclosporin A is soluble in ethanol (also DMSO) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect but it is typically used at around 100 nM.

TPA (12-O-Tetradecanoylphorbol-13-Acetate), 1 mg 1 mg

价格:¥

货号:4174S
品牌:CST

经销:基因有限公司

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate)是最常用的佛波酯。它结合并激活蛋白激酶C,对很多细胞和组织产生影响(1,2)。TPA已被证明在在小鼠皮肤上是一个强有力的促癌物(3)。相反,它已被证明是粒细胞白血病的一种有效的治疗药物,在大肠癌治疗中也有潜在疗效(4-5)。TPA也被证明能在实质性癌症患者中增加白细胞和中性粒细胞(6)。TPA以1mg粉末提供。储存在-20℃。使用前,将粉末溶解
1 mg. TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).
TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

Vatalanib 5mg

价格:¥

货号:12998S
品牌:CST

经销:基因有限公司

瓦他拉尼,5mg,多靶酪氨酸酶抑制剂
Vatalanib,5mg

Lipopolysaccharides(LPS) 脂多糖

价格:¥

货号:14011S
品牌:CST

经销:基因有限公司

10 mg. Lipopolysaccharide (LPS), also known as endotoxin, is a major glycolipid constituent of the outer cell wall of gram-negative bacteria. LPS molecules typically consist of a strain-specific distal polysaccharide side chain known as the O-antigen, a hydrophilic core oligosaccharide, and a hydrophobic domain referred to as lipid A. Lipid A is covalently bound to the outer bacterial membrane and is responsible for the toxicity of LPS (1-3). LPS is a potent activator of the proinflammatory response in many mammalian cell types, including macrophages, monocytes, and endothelial cells. Investigators have demonstrated that LPS binds to the CD14/TLR4/MD2 receptor complex, which in turn induces inflammatory cytokines including TNF-α, Interleukin-1, and IFN-α, as well as numerous inflammatory proteins such as iNOS, NF-κB, RIG-1, and IRF-3
For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml sterile PBS. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 µg/ml for 15 min-24 hours. Soluble in PBS and cell culture medium at 5 mg/ml and 1 mg/ml, respectively.

Erlotinib, 10 mg 10 mg

价格:¥

货号:5083S
品牌:CST

经销:基因有限公司

厄洛替尼是一种新的和有效的EGFR激酶通路ATP竞争性抑制剂。它在体外抑制EGFR的自磷酸化,并抑制纯化的EGFR激酶,其IC50分别为20nM和2nM(1)。厄洛替尼对EGFR的选择性比c-src和v-abl (1), ErbB-2和ErbB-4 (2)高出1000倍。有研究显示,厄洛替尼在多种细胞类型中抑制生长并诱导G1细胞周期阻滞,这些细胞有许多是过量表达EGFR(1,3-5)。提供厄洛替尼的冻干粉末。将其10mg重新溶解在2.54 ml DMSO中,即为10 mM储存液。工作浓度和处理时间可以根据
>99% 10 mg. Erlotinib is a novel and potent ATP-competitive inhibitor of the EGFR kinase pathway. It inhibited EGFR autophosphorylation with an IC50 of 20 nM in vitro and inhibit purified EGFR kinase with an IC50 of 2 nM (1). Erlotonib is greater than 1000-fold more selective for EGFR than c-src and v-abl (1), ErbB-2, and ErbB-4 (2). Studies have shown that erlotinib inhibits growth and induces G1 cell cycle arrest in multiple cell types, many of which overexpress EGFR
Erlotinib is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.54 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 0.1-10 µM for 0.5-2 hours prior to treating with a stimulator. It can also be used alone, with varying treatment times lasting up to 24 hours. Soluble in DMSO at 100 mg/ml, soluble in ethanol at 10 mg/ml with warming; very poorly soluble in water with a maximum solubility ~5-20 µM.

Nocodazole, 10 mg 10 mg

价格:¥

货号:2190S
品牌:CST

经销:基因有限公司

诺考达唑是一种抗肿瘤药物,能可逆地干扰微管的聚合(1)。它作为一种细胞周期同步化药物广泛用于细胞生物学实验室,能够诱导有丝分裂的停止,研究者已经证实高浓度的诺考达唑能诱导微管解聚,而低浓度时能改变纺锤体微管动力学,但微管不解聚(2-4)。最近的研究显示诺考达唑是一种常用的多种癌症相关激酶的抑制剂,包括ABL, c-KIT, BRAF, MEK1, MEK2和MET。提供的诺考达唑为冻干粉。对于1mg/ml浓度贮存液,在10 mg冻干粉中加入10 ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,
>97% 10 mg. C14H11N3O3S. Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET
Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

Oligomycin, 5 mg 5 mg

价格:¥

货号:9996L
品牌:CST

经销:基因有限公司

寡霉素,是一种淀粉酶产色链丝菌产生的抗生素,它可以通过结合到ATP合酶上的F0组分来抑制其活性,并阻断氧化磷酸化过程(1-3)。寡霉素在二甲亚砜(DMSO),丙酮和乙醇中可溶。
Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).
Oligomycin is soluble in DMSO, acetone or ethanol.

Brefeldin A 5 mg

价格:¥

货号:9972S
品牌:CST

经销:基因有限公司

Brefeldin A (BFA)是一个真菌代谢物,其被证明可逆地干扰从内质网向高尔基体转运(1,2)。当起初分离的作为一个抗生素(3),并且有一个广泛的抗生素活性,它主要作为研究蛋白转运的生物研究工具。BFA处理后导致在内质网上一个快速的蛋白堆积,并且高尔基体会被破坏。BFA的处理也能抑制蛋白分泌(4)和延长的给药时间能诱导凋亡(5)。BFA的主要目标出现是ADP-ribosylation factor (ARF),该蛋白复制对外壳蛋白到高尔基膜的关联(6,7)。5 mg粉末。存储-20ºC。Brefe
5 mg. Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulum to the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7).
Brefeldin A is supplied as a 5 mg powder. Store at -20ºC. Brefeldin A is soluble in DMSO (also ethanol and methanol) and stock solutions (typically 10 mg/ml) should be stored at -20ºC. Working concentrations and length of treatment can vary depending on desired effect. Inhibiton of ER to Golgi trafficking was observed as low as 100 ng/ml and apoptosis was observed with prolonged treatment at 10 μg/ml.

Cycloheximide, 1g 1KIT

价格:¥

货号:2112S
品牌:CST

经销:基因有限公司

环己酰亚胺,蛋白合成抑制剂,1g
Cycloheximide, 1g

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