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产品名/货号/品牌 简介 包装  
Monarch 质粒小提纯化柱:Monarch Plasmid Miniprep Columns, 100 columns Kit

价格:¥

货号:T1017L
品牌:NEB

经销:基因有限公司

Monarch 质粒小提试剂盒:Monarch Plasmid Miniprep Kit, 50 preps Kit

价格:¥

货号:T1010S
品牌:NEB

经销:基因有限公司

Monarch质粒小提试剂盒 The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch 质粒小提试剂盒:Monarch Plasmid Miniprep Kit, 250 preps Kit

价格:¥

货号:T1010L
品牌:NEB

经销:基因有限公司

Monarch质粒小提试剂盒 The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

NucleoSnip 质粒小抽试剂盒:NucleoSpin Plasmid 10 preps

价格:¥

货号:740588.10
品牌:MNG

经销:基因有限公司

快速,小规模制备高达50μg测序级别高纯度的质粒DNA。25分钟内可完成18个Miniprep。载量高达50ug。硅胶膜技术,Mini spin columns,2-10mL大肠杆菌培养物,常规产量:25-45ug。适用载体大小 <15 kbp。A260/A280:1.80–1.85。洗脱体积50 μL。应用范围:大肠杆菌高拷贝质粒提取,低拷贝质粒、P1 载体或粘粒提取,革兰氏阳性细菌抽提质粒DNA;纯化质粒DNA。产物可用于克隆,测序,PCR,转化和限制性分析等
Sequencing-grade plasmid DNA. Silica-membrane technology. Mini spin columns.
Sample material: 2–10 mL E. coli culture.
Vector size < 15 kbp
Typical yield: 25–45 µg
A260/A280: 1.80–1.85
Elution volume: 50 µL
Preparation time: 25 min/18 preps
Binding capacity: 50 µg
NucleoSpin® Plasmid Columns, Collection Tubes (2 mL), buffers, RNase A
10 preps for the isolation of plasmid DNA - NucleoSpin Plasmid Columns, Collection Tubes, buffers, RNase A

NucleoSnip 质粒小抽试剂盒(无盖):NucleoSpin Plasmid (NoLid) 10 preps

价格:¥

货号:740499.10
品牌:MNG

经销:基因有限公司

(10) 10 preps for the isolation of plasmid DNA - NucleoSpin Plasmid (NoLid) Columns, Collection Tubes, buffers, RNase A

NucleoSpin 质粒小抽试剂盒 EasyPure:NucleoSpin Plasmid EasyPure 10 preps

价格:¥

货号:740727.10
品牌:MNG

经销:基因有限公司

NucleoSpin 质粒小抽试剂盒 EasyPure:NucleoSpin Plasmid EasyPure 50 preps

价格:¥

货号:740727.50
品牌:MNG

经销:基因有限公司

NucleoBond 专用滤纸 XL:NucleoBond Folded Filters XL 50 preps

价格:¥

货号:740577
品牌:MNG

经销:基因有限公司

folded filters for NucleoBond AX 500 and AX 2000 Columns pack of 50

NucleoSnip 质粒小抽试剂盒:NucleoSpin Plasmid 50 preps

价格:¥

货号:740588.50
品牌:MNG

经销:基因有限公司

快速,小规模制备高达50μg测序级别高纯度的质粒DNA。25分钟内可完成18个Miniprep。载量高达50ug。硅胶膜技术,Mini spin columns,2-10mL大肠杆菌培养物,常规产量:25-45ug。适用载体大小 <15 kbp。A260/A280:1.80–1.85。洗脱体积50 μL。应用范围:大肠杆菌高拷贝质粒提取,低拷贝质粒、P1 载体或粘粒提取,革兰氏阳性细菌抽提质粒DNA;纯化质粒DNA。产物可用于克隆,测序,PCR,转化和限制性分析等
Sequencing-grade plasmid DNA. Silica-membrane technology. Mini spin columns.
Sample material: 2–10 mL E. coli culture.
Vector size < 15 kbp
Typical yield: 25–45 µg
A260/A280: 1.80–1.85
Elution volume: 50 µL
Preparation time: 25 min/18 preps
Binding capacity: 50 µg
NucleoSpin® Plasmid Columns, Collection Tubes (2 mL), buffers, RNase A
50 preps for the isolation of plasmid DNA - NucleoSpin Plasmid Columns, Collection Tubes, buffers, RNase A

NucleoSnip 质粒小抽试剂盒(无盖):NucleoSpin Plasmid (NoLid) 50 preps

价格:¥

货号:740499.50
品牌:MNG

经销:基因有限公司

快速,小规模制备高达50μg测序级别高纯度的质粒DNA。25分钟内可完成18个Miniprep。载量高达50ug。硅胶膜技术,Mini spin columns,2-10mL大肠杆菌培养物,常规产量:25-45ug。适用载体大小 <15 kbp。A260/A280:1.80–1.85。洗脱体积50 μL。应用范围:大肠杆菌高拷贝质粒提取,低拷贝质粒、P1 载体或粘粒提取,革兰氏阳性细菌抽提质粒DNA;纯化质粒DNA。产物可用于克隆,测序,PCR,转化和限制性分析等
Sequencing-grade plasmid DNA. Silica-membrane technology. Mini spin columns.
Sample material: 2–10 mL E. coli culture.
Vector size < 15 kbp
Typical yield: 25–45 µg
A260/A280: 1.80–1.85
Elution volume: 50 µL
Preparation time: 25 min/18 preps
Binding capacity: 50 µg
NucleoSpin® Plasmid Columns (NoLid), Collection Tubes (2 mL), buffers, RNase A
(50) 50 preps for the isolation of plasmid DNA - NucleoSpin Plasmid (NoLid) Columns, Collection Tubes, buffers, RNase A

NucleoBond 超大质粒提取试剂盒 10 preps

价格:¥

货号:740579
品牌:MNG

经销:基因有限公司

10 preps for the isolation of low-copy plasmid DNA - NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A

NucleoSpin 质粒小抽试剂盒 EasyPure:NucleoSpin Plasmid EasyPure 250 preps

价格:¥

货号:740727.250
品牌:MNG

经销:基因有限公司

NucleoSnip 质粒小抽试剂盒:NucleoSpin Plasmid 250 preps

价格:¥

货号:740588.250
品牌:MNG

经销:基因有限公司

快速,小规模制备高达50μg测序级别高纯度的质粒DNA。25分钟内可完成18个Miniprep。载量高达50ug。硅胶膜技术,Mini spin columns,2-10mL大肠杆菌培养物,常规产量:25-45ug。适用载体大小 <15 kbp。A260/A280:1.80–1.85。洗脱体积50 μL。应用范围:大肠杆菌高拷贝质粒提取,低拷贝质粒、P1 载体或粘粒提取,革兰氏阳性细菌抽提质粒DNA;纯化质粒DNA。产物可用于克隆,测序,PCR,转化和限制性分析等
Sequencing-grade plasmid DNA. Silica-membrane technology. Mini spin columns.
Sample material: 2–10 mL E. coli culture.
Vector size < 15 kbp
Typical yield: 25–45 µg
A260/A280: 1.80–1.85
Elution volume: 50 µL
Preparation time: 25 min/18 preps
Binding capacity: 50 µg
NucleoSpin® Plasmid Columns, Collection Tubes (2 mL), buffers, RNase A
250 preps for the isolation of plasmid DNA - NucleoSpin Plasmid Columns, Collection Tubes, buffers, RNase A

NucleoSnip 质粒小抽试剂盒(无盖):NucleoSpin Plasmid (NoLid) 250 preps

价格:¥

货号:740499.250
品牌:MNG

经销:基因有限公司

快速,小规模制备高达50μg测序级别高纯度的质粒DNA。25分钟内可完成18个Miniprep。载量高达50ug。硅胶膜技术,Mini spin columns,2-10mL大肠杆菌培养物,常规产量:25-45ug。适用载体大小 <15 kbp。A260/A280:1.80–1.85。洗脱体积50 μL。应用范围:大肠杆菌高拷贝质粒提取,低拷贝质粒、P1 载体或粘粒提取,革兰氏阳性细菌抽提质粒DNA;纯化质粒DNA。产物可用于克隆,测序,PCR,转化和限制性分析等
Sequencing-grade plasmid DNA. Silica-membrane technology. Mini spin columns.
Sample material: 2–10 mL E. coli culture.
Vector size < 15 kbp
Typical yield: 25–45 µg
A260/A280: 1.80–1.85
Elution volume: 50 µL
Preparation time: 25 min/18 preps
Binding capacity: 50 µg
NucleoSpin® Plasmid Columns (NoLid), Collection Tubes (2 mL), buffers, RNase A
(250) 250 preps for the isolation of plasmid DNA - NucleoSpin Plasmid (NoLid) Columns, Collection Tubes, buffers, RNase A

NucleoSpin (芯盒)质粒提取试剂盒(96孔板):NucleoSpin 96 Plasmid Core Kit 24*96 preps

价格:¥

货号:740616.24
品牌:MNG

经销:基因有限公司

ISOLATE II 质粒提取试剂盒:ISOLATE II Plasmid Mini Kit 10 preps

价格:¥

货号:BIO-52055
品牌:Bioline

经销:基因有限公司

硅胶膜技术,快速从1-5ml培养物(低拷贝10ml)中提取小量质粒DNA(质粒大小不超过15kb)。硅胶膜纯化柱最高载量达60ug。操作简单,25分钟完成18个样品制备,质粒产物纯度高,可进行PCR,测序,定量PCR,克隆等后继实验。For the rapid and efficient isolation of highly pure and maximal yields of plasmid DNA from bacterial cultures using silica-membrane spin column technology.

Rapid protocol - 25 minutes/18 preps
High-capacity - up to 40µg from 10ml of culture
High-purity plasmid DNA - typical A260/A280 ratio >1.8
Universal - protocols for both high-copy and low-copy plasmid

Applications
Isolation of high-copy plasmid DNA
Isolation of low-copy plasmid DNA, P1 constructs and cosmids
Isolation of plasmid DNA from Gram-positive bacteria
Plasmid DNA clean-up
Isolated DNA is ready for downstream applications such as PCR, real-time PCR, cloning and sequencing.

Description
ISOLATE II Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using silica-membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria, followed by clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants are efficiently removed during the washing steps. In the final step, highly-pure plasmid DNA is eluted. The kit shows excellent recovery of plasmid DNA and reproducible results (fig. 1) even from low culture volumes (fig. 2).
Separate protocols are provided for the isolation of high copy and low copy plasmids. In each case, intact, high purity plasmid DNA is isolated within 25 minutes. Typically, up to 25µg and 40µg plasmid DNA is obtained from 5ml and 10ml cultures, respectively.
The isolated DNA shows excellent performance in downstream molecular biology applications such as PCR, transformation, cloning, sequencing (fig. 3), restriction analysis, etc.
The online product manual has protocols for isolation of low-copy plasmids, P1 constructs and cosmid DNA from E. coli, isolation of plasmid DNA from Gram-positive bacteria as well as plasmid DNA clean-up. Frequent use of protocols for isolation of low-copy plasmids, P1 constructs and cosmids will require increased buffer volumes. See seperate ISOLATE II Plasmid Buffer Set (BIO-52078) for further details.

ISOLATE II 质粒提取试剂盒:ISOLATE II Plasmid Mini Kit 50 preps

价格:¥

货号:BIO-52056
品牌:Bioline

经销:基因有限公司

硅胶膜技术,快速从1-5ml培养物(低拷贝10ml)中提取小量质粒DNA(质粒大小不超过15kb)。硅胶膜纯化柱最高载量达60ug。操作简单,25分钟完成18个样品制备,质粒产物纯度高,可进行PCR,测序,定量PCR,克隆等后继实验。For the rapid and efficient isolation of highly pure and maximal yields of plasmid DNA from bacterial cultures using silica-membrane spin column technology.

Rapid protocol - 25 minutes/18 preps
High-capacity - up to 40µg from 10ml of culture
High-purity plasmid DNA - typical A260/A280 ratio >1.8
Universal - protocols for both high-copy and low-copy plasmid

Applications
Isolation of high-copy plasmid DNA
Isolation of low-copy plasmid DNA, P1 constructs and cosmids
Isolation of plasmid DNA from Gram-positive bacteria
Plasmid DNA clean-up
Isolated DNA is ready for downstream applications such as PCR, real-time PCR, cloning and sequencing.

Description
ISOLATE II Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using silica-membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria, followed by clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants are efficiently removed during the washing steps. In the final step, highly-pure plasmid DNA is eluted. The kit shows excellent recovery of plasmid DNA and reproducible results (fig. 1) even from low culture volumes (fig. 2).
Separate protocols are provided for the isolation of high copy and low copy plasmids. In each case, intact, high purity plasmid DNA is isolated within 25 minutes. Typically, up to 25µg and 40µg plasmid DNA is obtained from 5ml and 10ml cultures, respectively.
The isolated DNA shows excellent performance in downstream molecular biology applications such as PCR, transformation, cloning, sequencing (fig. 3), restriction analysis, etc.
The online product manual has protocols for isolation of low-copy plasmids, P1 constructs and cosmid DNA from E. coli, isolation of plasmid DNA from Gram-positive bacteria as well as plasmid DNA clean-up. Frequent use of protocols for isolation of low-copy plasmids, P1 constructs and cosmids will require increased buffer volumes. See seperate ISOLATE II Plasmid Buffer Set (BIO-52078) for further details.

ISOLATE II 质粒提取试剂盒:ISOLATE II Plasmid Mini Kit 250 preps

价格:¥

货号:BIO-52057
品牌:Bioline

经销:基因有限公司

硅胶膜技术,快速从1-5ml培养物(低拷贝10ml)中提取小量质粒DNA(质粒大小不超过15kb)。硅胶膜纯化柱最高载量达60ug。操作简单,25分钟完成18个样品制备,质粒产物纯度高,可进行PCR,测序,定量PCR,克隆等后继实验。For the rapid and efficient isolation of highly pure and maximal yields of plasmid DNA from bacterial cultures using silica-membrane spin column technology.

Rapid protocol - 25 minutes/18 preps
High-capacity - up to 40µg from 10ml of culture
High-purity plasmid DNA - typical A260/A280 ratio >1.8
Universal - protocols for both high-copy and low-copy plasmid

Applications
Isolation of high-copy plasmid DNA
Isolation of low-copy plasmid DNA, P1 constructs and cosmids
Isolation of plasmid DNA from Gram-positive bacteria
Plasmid DNA clean-up
Isolated DNA is ready for downstream applications such as PCR, real-time PCR, cloning and sequencing.

Description
ISOLATE II Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using silica-membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria, followed by clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants are efficiently removed during the washing steps. In the final step, highly-pure plasmid DNA is eluted. The kit shows excellent recovery of plasmid DNA and reproducible results (fig. 1) even from low culture volumes (fig. 2).
Separate protocols are provided for the isolation of high copy and low copy plasmids. In each case, intact, high purity plasmid DNA is isolated within 25 minutes. Typically, up to 25µg and 40µg plasmid DNA is obtained from 5ml and 10ml cultures, respectively.
The isolated DNA shows excellent performance in downstream molecular biology applications such as PCR, transformation, cloning, sequencing (fig. 3), restriction analysis, etc.
The online product manual has protocols for isolation of low-copy plasmids, P1 constructs and cosmid DNA from E. coli, isolation of plasmid DNA from Gram-positive bacteria as well as plasmid DNA clean-up. Frequent use of protocols for isolation of low-copy plasmids, P1 constructs and cosmids will require increased buffer volumes. See seperate ISOLATE II Plasmid Buffer Set (BIO-52078) for further details.

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