The second generation for rapid isolation of genomic DNA from plant and fungi samples with higher yield and quality • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters included for clarification of lysate • Improved buffer compositions → higher yield and purity • Optimized silica membrane → improved DNA binding • RNase A included
Applications • DNA from plant cells and tissue • DNA from fungi • Typical downstream applications: PCR, Southern blotting, enzymatic reactions NucleoSpin Plant® II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A 10 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase A
For rapid medium and large scale preparation of genomic DNA from plant cells and fungi • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters L / XL included for clarification of lysate • Improved buffer compositions → higher yield and purity • RNase A included NucleoSpin® Plant II Maxi Columns with Collection Tubes, NucleoSpin® Filters XL, Collection Tubes (50 mL), buffers, RNase A 10 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Maxi Columns, NucleoSpin Filters XL, Collection Tubes, buffers, RNase A
For rapid medium and large scale preparation of genomic DNA from plant cells and fungi • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters L / XL included for clarification of lysate • Improved buffer compositions → higher yield and purity • RNase A included NucleoSpin® Plant II Midi Columns with Collection Tubes, NucleoSpin® Filters L, Collection Tubes (15 mL), buffers, RNase A 20 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Midi Columns, NucleoSpin Filters L, Collection Tubes, buffers, RNase A
The second generation for rapid isolation of genomic DNA from plant and fungi samples with higher yield and quality • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters included for clarification of lysate • Improved buffer compositions → higher yield and purity • Optimized silica membrane → improved DNA binding • RNase A included
Applications • DNA from plant cells and tissue • DNA from fungi • Typical downstream applications: PCR, Southern blotting, enzymatic reactions NucleoSpin Plant® II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A 50 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase A
Magnetic-bead based isolation of genomic DNA from plant tissue. Manual or automated • One-tube procedure minimizes risk of cross-contamination • Small elution volumes ≥ 50 μL possible • Yield does not depend on elution volume • Easily adapted to automated use
Technology: Magnetic-bead technology Format: Highly reactive superparamagnetic beads Sample material: 20–50 mg plant tissue Fragment size: 300 bp–approx. 50 kbp Typical yield: 10–20 µg A260/A280: 1.6–1.9 Elution volume: 50 µL Preparation time: 120 min/96 preps Binding capacity: ≈ 0.4 µg/µL beads • Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction NucleoMag® C-Beads, Elution Plate U-bottom, buffers, RNase A 96 preps for the purification of genomic DNA from plant - NucleoMag C-Beads, Elution Plate U-bottom, buffers, RNase A
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin® Plant II Binding Strips, MN Wash Plates, MN square-well Block, Racks of Tube Strips, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A 12 x 8 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Strips, MN Wash Plate, Racks of Tube Strips, MN Square-well Blocks, Gas-permeable Foil, buffers, RNase A
The second generation for rapid isolation of genomic DNA from plant and fungi samples with higher yield and quality • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters included for clarification of lysate • Improved buffer compositions → higher yield and purity • Optimized silica membrane → improved DNA binding • RNase A included
Applications • DNA from plant cells and tissue • DNA from fungi • Typical downstream applications: PCR, Southern blotting, enzymatic reactions NucleoSpin Plant® II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A 250 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase A
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin Plant Binding Plates, MN Square-well Blocks, Racks with Tube Strips, Cap Strips,Gas-permeable Foil, buffers, RNase A 2 x 96 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Plates, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Gas-permeable Foil, buffers, RNase A
Magnetic-bead based isolation of genomic DNA from plant tissue. Manual or automated • One-tube procedure minimizes risk of cross-contamination • Small elution volumes ≥ 50 μL possible • Yield does not depend on elution volume • Easily adapted to automated use
Technology: Magnetic-bead technology Format: Highly reactive superparamagnetic beads Sample material: 20–50 mg plant tissue Fragment size: 300 bp–approx. 50 kbp Typical yield: 10–20 µg A260/A280: 1.6–1.9 Elution volume: 50 µL Preparation time: 120 min/96 preps Binding capacity: ≈ 0.4 µg/µL beads • Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction NucleoMag® C-Beads, Elution Plate U-bottom, buffers, RNase A 4 x 96 preps for the purification of genomic DNA from plant - NucleoMag C-Beads, Elution Plate U-bottom, buffers, RNase A
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin® Plant II Binding Strips, buffers, RNase A 48 x 8 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Strips, buffers, RNase A
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin® Plant II Binding Plates, buffers, RNase A 4 x 96 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Plates, buffers, RNase A no dangerous goods according transport regulations
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin Plant Binding Plates, MN Square-well Blocks, Racks with Tube Strips, Cap Strips,Gas-permeable Foil, buffers, RNase A 4 x 96 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Plates, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Gas-permeable Foils, buffers, RNase A no dangerous goods accordin
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin® Plant II Binding Strips, MN Wash Plates, MN square-well Block, Racks of Tube Strips, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A 60 x 8 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Strips, MN Wash Plate, Racks of Tube Strips, MN Square-well Blocks, Gas-permeable Foils, buffers, RNase A
Magnetic-bead based isolation of genomic DNA from plant tissue. Manual or automated • One-tube procedure minimizes risk of cross-contamination • Small elution volumes ≥ 50 μL possible • Yield does not depend on elution volume • Easily adapted to automated use
Technology: Magnetic-bead technology Format: Highly reactive superparamagnetic beads Sample material: 20–50 mg plant tissue Fragment size: 300 bp–approx. 50 kbp Typical yield: 10–20 µg A260/A280: 1.6–1.9 Elution volume: 50 µL Preparation time: 120 min/96 preps Binding capacity: ≈ 0.4 µg/µL beads • Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction NucleoMag® C-Beads, Elution Plate U-bottom, buffers, RNase A 24 x 96 preps for the purification of genomic DNA from plant - NucleoMag C-Beads, Elution Plates U-bottom, Buffers, RNase A
Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of genomic DNA from plant cells and tissue • Processing possible under vacuum* or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • DNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms. NucleoSpin Plant Binding Plates, MN Square-well Blocks, Racks with Tube Strips, Cap Strips,Gas-permeable Foil, buffers, RNase A 24 x 96 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Binding Plates, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Gas-permeable Foils, buffers, RNase A no dangerous goods accordi
植物DNA提取试剂盒（10次），30分钟以内提取基因组DNA，内含两种裂解液可满足不同样本裂解要求；提供Filter，有效去除裂解液中杂质 快速从各种植物组织中提取高纯度基因组。硅胶膜纯化技术快速简便，提供过滤器shredder，30分钟内完成提取。DNA for the rapid purification of highest quality genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots and fruits, as well as dung, animal-fecal, soil and compost samples.
Fast - plant genomic DNA isolated in 30 minutes
High-purity DNA - typical A260/A280 ratio 1.6 - 1.9
Choice of two optimized lysis buffers - for all sample types
Filters (shredders) included - for clarification of lysate
Applications Isolation of genomic DNA from:
Fresh/frozen/lyophilized plant tissue
Dung, animal fecal, soil and compost samples
Herbarium specimens
Fungi
Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping.
Description
ISOLATE II Plant DNA Kit is designed for the rapid purification of genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots, fruits, etc. Up to 100mg wet plant material and up to 20mg dry plant material can be processed per spin column.
The ISOLATE II protocol does not require the use of Proteinase K, which means that all components can be conveniently stored at room temperature.
Plant samples are homogenized by mechanical treatment. DNA is then extracted with chaotropic salts, denaturing agents and detergents. Crude lysates are cleared in order to remove polysaccharides, contaminants and residual cellular debris. The DNA is bound to a silica membrane, washed and the pure genomic DNA is eluted.
The kit contains two optimized lysis buffers based on the established CTAB and SDS methods. RNase A is included to remove RNA.
ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA with a variety of homogenization techniques (fig. 1). High yields are obtained with every miniprep (fig. 2).
The online product manual has protocols for purifying plant genomic DNA, fungal genomic DNA, dung, animal fecal, soil and compost samples. The isolated DNA is ready for use in downstream applications such as PCR, real-time PCR, genotyping, restriction analysis and sequencing.
快速从各种植物组织中提取高纯度基因组。硅胶膜纯化技术快速简便，提供过滤器shredder，30分钟内完成提取。DNA for the rapid purification of highest quality genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots and fruits, as well as dung, animal-fecal, soil and compost samples.
Fast - plant genomic DNA isolated in 30 minutes
High-purity DNA - typical A260/A280 ratio 1.6 - 1.9
Choice of two optimized lysis buffers - for all sample types
Filters (shredders) included - for clarification of lysate
Applications Isolation of genomic DNA from:
Fresh/frozen/lyophilized plant tissue
Dung, animal fecal, soil and compost samples
Herbarium specimens
Fungi
Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping.
Description
ISOLATE II Plant DNA Kit is designed for the rapid purification of genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots, fruits, etc. Up to 100mg wet plant material and up to 20mg dry plant material can be processed per spin column.
The ISOLATE II protocol does not require the use of Proteinase K, which means that all components can be conveniently stored at room temperature.
Plant samples are homogenized by mechanical treatment. DNA is then extracted with chaotropic salts, denaturing agents and detergents. Crude lysates are cleared in order to remove polysaccharides, contaminants and residual cellular debris. The DNA is bound to a silica membrane, washed and the pure genomic DNA is eluted.
The kit contains two optimized lysis buffers based on the established CTAB and SDS methods. RNase A is included to remove RNA.
ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA with a variety of homogenization techniques (fig. 1). High yields are obtained with every miniprep (fig. 2).
The online product manual has protocols for purifying plant genomic DNA, fungal genomic DNA, dung, animal fecal, soil and compost samples. The isolated DNA is ready for use in downstream applications such as PCR, real-time PCR, genotyping, restriction analysis and sequencing.
快速从各种植物组织中提取高纯度基因组。硅胶膜纯化技术快速简便，提供过滤器shredder，30分钟内完成提取。DNA for the rapid purification of highest quality genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots and fruits, as well as dung, animal-fecal, soil and compost samples.
Fast - plant genomic DNA isolated in 30 minutes
High-purity DNA - typical A260/A280 ratio 1.6 - 1.9
Choice of two optimized lysis buffers - for all sample types
Filters (shredders) included - for clarification of lysate
Applications Isolation of genomic DNA from:
Fresh/frozen/lyophilized plant tissue
Dung, animal fecal, soil and compost samples
Herbarium specimens
Fungi
Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping.
Description
ISOLATE II Plant DNA Kit is designed for the rapid purification of genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots, fruits, etc. Up to 100mg wet plant material and up to 20mg dry plant material can be processed per spin column.
The ISOLATE II protocol does not require the use of Proteinase K, which means that all components can be conveniently stored at room temperature.
Plant samples are homogenized by mechanical treatment. DNA is then extracted with chaotropic salts, denaturing agents and detergents. Crude lysates are cleared in order to remove polysaccharides, contaminants and residual cellular debris. The DNA is bound to a silica membrane, washed and the pure genomic DNA is eluted.
The kit contains two optimized lysis buffers based on the established CTAB and SDS methods. RNase A is included to remove RNA.
ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA with a variety of homogenization techniques (fig. 1). High yields are obtained with every miniprep (fig. 2).
The online product manual has protocols for purifying plant genomic DNA, fungal genomic DNA, dung, animal fecal, soil and compost samples. The isolated DNA is ready for use in downstream applications such as PCR, real-time PCR, genotyping, restriction analysis and sequencing.
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