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NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 10 preps

价格:¥

货号:740955.10
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 10 preps

价格:¥

货号:740902.10
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。<5 mg组织, 1-100000个细胞。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
10 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

NucleoSpin RNA 提取试剂盒 Midi:NucleoSpin RNA Midi 20 ml

价格:¥

货号:740962.20
品牌:MNG

经销:基因有限公司

从组织和细胞中快速提取6-700ug的最完整的RNA。离心柱式,载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解,有效去除DNA污染。
Midi spin kit for the isolation of RNA of highest integrity
• Efficient removal of genomic DNA – rDNase included for on-column digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders)
included
• Up to 600 μg ready-to-use RNA

Technology: Silica-membrane technology
Format: Midi spin columns
Sample material: < 5 x 10(7) cultured cells, < 10(10) bacterial cells, < 3 x 10(8) yeast cells, < 200 mg tissue
Fragment size: 200 b
Typical yield: 180 µg from 10(7) HeLa cells, 620 µg from 4 x 10(7) HeLa cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 500 µL
Preparation time: 80 min/4 preps
Binding capacity: 700 µg

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin® RNA Midi Columns with Collection Tubes(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase
20 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA L Columns, NucleoSpin RNA L Filters, Elution Tubes, buffers, RNase-free rDNase

NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 50 preps

价格:¥

货号:740955.50
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 50 preps

价格:¥

货号:740902.50
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
50 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 250 preps

价格:¥

货号:740955.250
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 250 preps

价格:¥

货号:740902.250
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
250 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

ISOLATE II RNA 提取试剂盒:ISOLATE II RNA Mini Kit 10 preps

价格:¥

货号:BIO-52071
品牌:Bioline

经销:基因有限公司

硅胶模离心柱快速纯化总RNA试剂盒,可从30mg组织或5x10(6)培养细胞中快速纯化多达200ug总RNA,也适用于体液,微生物,石蜡包埋样本,RNAlater样本。试剂盒含纯化柱,过滤柱,缓冲液和DNAseI,洗脱体积可少至40ul,30分钟内可同时完成6个样本制备。For fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast, bacteria, biological fluids, paraffin embedded tissue, RNAlater® treated samples, as well as other starting materials.

•Rapid protocol - 30 min/6 preps
•High purity RNA - typical A260/A280 ratio 1.9 - 2.1
•High quality - RNA integrity number (RIN) >9
•Filters (shredders) included - to enhance sample homogenization
•DNase I included - for complete removal of genomic DNA

Applications Isolation of RNA from: •Mammalian tissues •Eukaryotic cells •Bacterial cells •Yeast •Biological fluids (cell-free) Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR. Description
ISOLATE II RNA Mini Kit is specially designed for fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae.

The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA. RNase-free DNase I is supplied with the kit.

There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted .

The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples, as well as DNase I treatment of purified RNA and clean-up of RNA from reaction mixtures.
RNA isolated with the ISOLATE II RNA Mini Kit shows excellent performance in downstream applications, such as reverse transcription, one-step real-time PCR, Northern blot analysis, microarrays and RNA protection assays.

ISOLATE II RNA 提取试剂盒(微量样本):ISOLATE II RNA Micro Kit 10 preps

价格:¥

货号:BIO-52074
品牌:Bioline

经销:基因有限公司

适合单细胞,显微切割,激光俘获,活检穿刺,流式分选细胞等微量样品的总RNA快速纯化试剂盒(硅胶模离心柱),40分钟可同时完成12个样品操作,载量90ugRNA,洗脱体积5-30ul. for the fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material includes tissue (of up to 5 mg) or small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, cultured cell pellets and flow cytometer sorted cells
•Rapid protocol - 40 min/12 preps
•Perfect for small samples - biopsy or single cells
•High yield and purity - typical A260/A280 ratio 1.9 - 2.1
•Extra filters (shredders) and DNase I included - for excellent RNA recovery

Applications: Isolation of RNA from small amounts of samples:
•Single cells
•Pellets of cultured cells
•Cryosections
•Laser-captured or flow sorted cells
•Small amounts of tissues e.g. biopsy materials
Isolated RNA is ready for downstream applications such as reverse transcription and real-time PCR.

Description
ISOLATE II RNA Micro Kit is specially designed for fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material comprises tissue (up to 5 mg), small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, pellets of cultured cells and flow cytometer sorted cells.

The step-by-step protocol is easy to follow. Cells are lysed with an optimized lysis buffer containing chaotropic ions which simultaneously inactivates RNases, thus preventing degradation of released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit).

There is no need to perform a separate DNase I digestion step. RNA is then bound to the silica membrane. The novel kit design allows elution of RNA in as little as 5–30μl. Simple wash steps remove remaining cell debris. Pure, highly concentrated RNA is eluted in the final step with RNase-free water .

Carrier RNA- poly(-A) RNA is included in the kit, which typically does not interfere with subsequent RT-PCR but allows for optimal performance with the smallest samples.

The isolated RNA shows excellent performance in downstream applications such as reverse transcription, primer extension, Real-Time PCR , Northern blot analysis, microarrays and RNA protection assays.

ISOLATE II RNA 提取试剂盒:ISOLATE II RNA Mini Kit 50 preps

价格:¥

货号:BIO-52072
品牌:Bioline

经销:基因有限公司

硅胶模离心柱快速纯化总RNA试剂盒,可从30mg组织或5x10(6)培养细胞中快速纯化多达200ug总RNA,也适用于体液,微生物,石蜡包埋样本,RNAlater样本。试剂盒含纯化柱,过滤柱,缓冲液和DNAseI,洗脱体积可少至40ul,30分钟内可同时完成6个样本制备。For fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast, bacteria, biological fluids, paraffin embedded tissue, RNAlater® treated samples, as well as other starting materials.

•Rapid protocol - 30 min/6 preps
•High purity RNA - typical A260/A280 ratio 1.9 - 2.1
•High quality - RNA integrity number (RIN) >9
•Filters (shredders) included - to enhance sample homogenization
•DNase I included - for complete removal of genomic DNA

Applications Isolation of RNA from: •Mammalian tissues •Eukaryotic cells •Bacterial cells •Yeast •Biological fluids (cell-free) Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR. Description
ISOLATE II RNA Mini Kit is specially designed for fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae.

The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA. RNase-free DNase I is supplied with the kit.

There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted .

The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples, as well as DNase I treatment of purified RNA and clean-up of RNA from reaction mixtures.
RNA isolated with the ISOLATE II RNA Mini Kit shows excellent performance in downstream applications, such as reverse transcription, one-step real-time PCR, Northern blot analysis, microarrays and RNA protection assays.

ISOLATE II RNA 提取试剂盒(微量样本):ISOLATE II RNA Micro Kit 50 preps

价格:¥

货号:BIO-52075
品牌:Bioline

经销:基因有限公司

适合单细胞,显微切割,激光俘获,活检穿刺,流式分选细胞等微量样品的总RNA快速纯化试剂盒(硅胶模离心柱),40分钟可同时完成12个样品操作,载量90ugRNA,洗脱体积5-30ul. for the fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material includes tissue (of up to 5 mg) or small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, cultured cell pellets and flow cytometer sorted cells
•Rapid protocol - 40 min/12 preps
•Perfect for small samples - biopsy or single cells
•High yield and purity - typical A260/A280 ratio 1.9 - 2.1
•Extra filters (shredders) and DNase I included - for excellent RNA recovery

Applications: Isolation of RNA from small amounts of samples:
•Single cells
•Pellets of cultured cells
•Cryosections
•Laser-captured or flow sorted cells
•Small amounts of tissues e.g. biopsy materials
Isolated RNA is ready for downstream applications such as reverse transcription and real-time PCR.

Description
ISOLATE II RNA Micro Kit is specially designed for fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material comprises tissue (up to 5 mg), small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, pellets of cultured cells and flow cytometer sorted cells.

The step-by-step protocol is easy to follow. Cells are lysed with an optimized lysis buffer containing chaotropic ions which simultaneously inactivates RNases, thus preventing degradation of released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit).

There is no need to perform a separate DNase I digestion step. RNA is then bound to the silica membrane. The novel kit design allows elution of RNA in as little as 5–30μl. Simple wash steps remove remaining cell debris. Pure, highly concentrated RNA is eluted in the final step with RNase-free water .

Carrier RNA- poly(-A) RNA is included in the kit, which typically does not interfere with subsequent RT-PCR but allows for optimal performance with the smallest samples.

The isolated RNA shows excellent performance in downstream applications such as reverse transcription, primer extension, Real-Time PCR , Northern blot analysis, microarrays and RNA protection assays.

ISOLATE II RNA 提取试剂盒:ISOLATE II RNA Mini Kit 250 preps

价格:¥

货号:BIO-52073
品牌:Bioline

经销:基因有限公司

硅胶模离心柱快速纯化总RNA试剂盒,可从30mg组织或5x10(6)培养细胞中快速纯化多达200ug总RNA,也适用于体液,微生物,石蜡包埋样本,RNAlater样本。试剂盒含纯化柱,过滤柱,缓冲液和DNAseI,洗脱体积可少至40ul,30分钟内可同时完成6个样本制备。For fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast, bacteria, biological fluids, paraffin embedded tissue, RNAlater® treated samples, as well as other starting materials.

•Rapid protocol - 30 min/6 preps
•High purity RNA - typical A260/A280 ratio 1.9 - 2.1
•High quality - RNA integrity number (RIN) >9
•Filters (shredders) included - to enhance sample homogenization
•DNase I included - for complete removal of genomic DNA

Applications Isolation of RNA from: •Mammalian tissues •Eukaryotic cells •Bacterial cells •Yeast •Biological fluids (cell-free) Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR. Description
ISOLATE II RNA Mini Kit is specially designed for fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae.

The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA. RNase-free DNase I is supplied with the kit.

There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted .

The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples, as well as DNase I treatment of purified RNA and clean-up of RNA from reaction mixtures.
RNA isolated with the ISOLATE II RNA Mini Kit shows excellent performance in downstream applications, such as reverse transcription, one-step real-time PCR, Northern blot analysis, microarrays and RNA protection assays.

QuickGene 手动 RNA细胞抽提试剂盒 (SP-RC):QuickGene SP kit RNA cultured cell (SP-RC) 96个样/kit

价格:¥

货号:40321300145
品牌:Kurabo

经销:基因有限公司

QuickGene SP kit RNA cultured cell (SP-RC) QuickGene 培养细胞RNA抽提试剂盒,96 prep。不依赖于KRB仪器,需离心机,每个制备适合10(6)个培养悬浮或贴壁细胞的RNA抽提。35分钟内可完成8个样本制备

QuickGene 手动 RNA细胞抽提试剂盒 (SP-RC2):QuickGene SP kit RNA cultured cell HC (SP-RC2 96个样/kit

价格:¥

货号:40321300147
品牌:Kurabo

经销:基因有限公司

QuickGene SP kit RNA cultured cell HC (SP-RC2) QuickGene 培养细胞RNA抽提试剂盒,96 prep(6cm dish培养细胞/3-15 × 10(6)悬浮细胞)或者48 prep(10cm dish).不依赖于KRB仪器,需离心机。55分钟可完成培养细胞的RNA抽提。

QuickGene手动 RNA组织抽提试剂盒 (SP-RT):QuickGene SP kit RNA tissue (SP-RT) 96个样/kit

价格:¥

货号:40321300155
品牌:Kurabo

经销:基因有限公司

QuickGene SP kit RNA tissue (SP-RT) QuickGene 组织RNA抽提试剂盒,96 prep。不依赖于KRB仪器,需离心机,每个制备的样本量5-30mg.用于动物组织RNA提取。30分钟可完成8个样本制备。

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