快速简便地从<400ul全血中提取高质量RNA。产量高，无需预处理红细胞，无DNA污染，兼容常规采血管和抗凝剂。 Isolation of RNA from fresh or frozen whole blood (human or animal) • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4°C – complete processing at room temperature) • Superior RNA yield and quality from up to 400 µL whole blood • Efficient on-column DNA removal for an increased sensitivity in downstream applications • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 400 µL whole blood (fresh or frozen) Fragment size: > 200 nt Typical yield: 1–8 µg (400 µL whole blood)** Typical purity (A260/A280) 1.9–2.1 Elution volume: 40–120 µL Preparation time: 55 min/6 preps Binding capacity: 200 µg Applications** • Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin) • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology NucleoSpin® RNA Blood Columns, Collection Tubes (2 mL), buffers, RNase-free rDNase, Liquid Proteinase K 10 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Columns, Collection Tubes, buffers, RNase-free rDNase, Liquid Proteinase K
快速简洁地从1.3ml全血中提取高质量的RNA。无需预处理红细胞，产量高，无DNA污染，兼容常规采血管抗凝剂 Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal) • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at room temperature) • Superior RNA yield and quality from up to 1.3 mL whole blood • Efficient on-column DNA removal for an increased sensitivity in downstream applications • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
Technology: Silica-membrane technology Format: Midi spin columns Sample material: 400–1300 µL whole blood (fresh or frozen) Fragment size: > 200 nt Typical yield: 4–26 µg (1300 µL whole blood)** Typical yield (A260/A280): 1.9–2.1 Elution volume: 200–400 µL Preparation time: 75 min/6 preps Binding capacity: 700 µg Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology NucleoSpin® RNA Blood Midi, Columns with Collection Tubes, Collection Tubes (15 mL), buffers, RNase-free rDNase, Liquid Proteinase K 20 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Midi Columns, Collection Tubes, buffers, RNase-free rDNase, Liquid Proteinase K
快速简便地从<400ul全血中提取高质量RNA。产量高，无需预处理红细胞，无DNA污染，兼容常规采血管和抗凝剂。 Isolation of RNA from fresh or frozen whole blood (human or animal) • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4°C – complete processing at room temperature) • Superior RNA yield and quality from up to 400 µL whole blood • Efficient on-column DNA removal for an increased sensitivity in downstream applications • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 400 µL whole blood (fresh or frozen) Fragment size: > 200 nt Typical yield: 1–8 µg (400 µL whole blood)** Typical purity (A260/A280) 1.9–2.1 Elution volume: 40–120 µL Preparation time: 55 min/6 preps Binding capacity: 200 µg Applications** • Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin) • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology NucleoSpin® RNA Blood Columns, Collection Tubes (2 mL), buffers, RNase-free rDNase, Liquid Proteinase K 50 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Columns, Collection Tubes, buffers, RNase-free rDNase, Liquid Proteinase K
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 12 x 8 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Binding Strips, MN Wash Plates, Racks of Tube Strips, Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 2 x 96 preps for the isolation of total RNA from blood - NucleoSpin RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 4 x 96 preps for the isolation of total RNA from blood - NucleoSpin RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 60 x 8 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Binding Strips, MN Wash Plates, Racks of Tube Strips, Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
高效快速从体液如血液，血清，血浆，尿液，唾液，CSF中分离高质量的总RNA，包括大分子RNA，各类小分子RNA和RNA病毒。适合低RNA含量样本，带DNAseI。for the rapid, phenol-free isolation of high quality and high yield total RNA from a wide range of biofluids including blood, serum, plasma, urine, saliva, CSF as well as RNA viruses.
•High quality total RNA - efficient recovery from challenging biofluids and viral samples
•High purity and yield - ideal for samples with low RNA content
•Complete RNA diversity - all sizes of RNA isolated, from large mRNA, viral RNA down to miRNA and siRNA
•Rapid, phenol-free protocol (20 min/10 preps) - increase your throughput
•DNase I included - efficient digestion of genomic DNA for sensitive applications
Applications Isolation of all sizes of RNA from:
•Biofluids including blood, serum, plasma, urine, saliva, CSF
•Viruses
Pure, isolated RNA ready for all downstream applications such as real-time RT-PCR, RNA-seq and microarrays.
Description
The ISOLATE II Biofluids RNA Kit is specially developed for the rapid isolation of high quality total RNA from cell-free biofluids and viruses. High yields of total RNA can be purified from blood (fig. 1), plasma, serum and other types of biofluids such as saliva, urine, semen and cerebrospinal fluid. Viral RNA can be isolated from the same sample sources, as well as cultured cells and tissue. The kit isolates all sizes of RNA from large mRNA, viral RNA and ribosomal RNA down to small RNAs such as miRNA and siRNA. The ISOLATE II Biofluids RNA Kit is compatible with very limited, or challenging sample sources containing low RNA content.
Isolation is based on a fast spin column format using a novel RNA affinity silica as the separation matrix. RNA molecules are preferentially purified from other cellular components such as proteins without the use of phenol or chloroform.
Samples are first lysed in the presence of guanidinium thiocyanate, a chaotropic salt which immediately deactivates endogenous RNases to ensure purification of intact RNA. DNA is then captured on a Genomic DNA Removal Column to remove contaminating genomic DNA. Ethanol is added to the flow-through and then applied to a RNA Column. The bound total RNA is washed to remove any impurities and eluted.
The purified RNA is of the highest purity and integrity and is ready for use in various applications, including Real-Time RT-PCR, RT-PCR, cDNA synthesis, RNA-seq and microarrays.
Components
Reagent 50 Reactions
ISOLATE II RNA Columns 50
ISOLATE II Genomic DNA Removal Columns 50
Collection Tubes (2ml) 100
Elution Tubes (1.7ml) 50
Lysis Buffer RX 40ml
Wash Buffer W1 38ml
DNase I Solution (RNase-free) 0.8ml
DNase I Reaction Buffer DRB 6ml
RNA Elution Buffer 6ml
Product Manual 1
Bench Protocol Sheet 1
Storage & StabilityThe DNase I Solution should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature.
When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
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