Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Parallel isolation of RNA and DNA from undivided samples in one working procedure
• To be used in combination with NucleoSpin® RNA II, NucleoSpin® RNA XS, NucleoSpin® miRNA, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein kits
• No need to split samples (e. g., precious, indivisible samples like biopsy material)
• High quality DNA and RNA from one sample, perfect for PCR, RT-PCR, real-time PCR
• Fast procedure

Format Buffer set for elution of DNA in combination with RNA preparations, to be used in combination with NucleoSpin® RNA kits
DNA size < 30 kbp
Typical DNA yield 5 µg from 106 HeLa cells; 16 µg from 30 mg pig liver; 5 µg from 100 mg maize leaf
A260/A280 1.7–2.0
DNA elution volume 100 µL
Applications*
• For biopsy material: RNA for expression analysis; DNA for mutation analysis (e.g., of cancer genes)
• For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (e.g., TBE-V, Tick-borne encephalitis virus, infectious agent of meningoencephalitis); DNA for analysis of germs (e.g., Borrelia burgdorferi, infective agent of the Lyme disease)
• For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or other genes; DNA for analysis of integration site, integration number and sequence confirmation of the transformed gene
• For transfected cultured cells: RNA for expression analysis of the transgene or other genes of interest; DNA for analysis of methylation status of the transgene or other genes of interest

Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase