Parallel isolation of RNA and protein from undivided samples • Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required • High RNA yield and integrity • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** Rapid purification of total RNA and protein from cultured cells and tissue • Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening • Broad spectrum of starting material tested and proteins already detected NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 10 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Parallel isolation of RNA, DNA, and protein from undivided samples • Convenient one-column preparation of RNA, DNA, and protein • High quality RNA and DNA, ready to use for downstream applications • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** • Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping • Reliable interpretation of RNA, DNA, and protein amounts • Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors) • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants • RNA and DNA are ready to use for all typical downstream applications • Proteins are ready to use for SDS-PAGE / Western blotting NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase 10 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Parallel isolation of RNA and DNA from undivided samples in one working procedure • To be used in combination with NucleoSpin® RNA II, NucleoSpin® RNA XS, NucleoSpin® miRNA, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein kits • No need to split samples (e. g., precious, indivisible samples like biopsy material) • High quality DNA and RNA from one sample, perfect for PCR, RT-PCR, real-time PCR • Fast procedure
Format Buffer set for elution of DNA in combination with RNA preparations, to be used in combination with NucleoSpin® RNA kits DNA size < 30 kbp Typical DNA yield 5 µg from 106 HeLa cells; 16 µg from 30 mg pig liver; 5 µg from 100 mg maize leaf A260/A280 1.7–2.0 DNA elution volume 100 µL Applications* • For biopsy material: RNA for expression analysis; DNA for mutation analysis (e.g., of cancer genes) • For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (e.g., TBE-V, Tick-borne encephalitis virus, infectious agent of meningoencephalitis); DNA for analysis of germs (e.g., Borrelia burgdorferi, infective agent of the Lyme disease) • For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or other genes; DNA for analysis of integration site, integration number and sequence confirmation of the transformed gene • For transfected cultured cells: RNA for expression analysis of the transgene or other genes of interest; DNA for analysis of methylation status of the transgene or other genes of interest
Parallel isolation of RNA and protein from undivided samples • Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required • High RNA yield and integrity • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** Rapid purification of total RNA and protein from cultured cells and tissue • Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening • Broad spectrum of starting material tested and proteins already detected NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Parallel isolation of RNA, DNA, and protein from undivided samples • Convenient one-column preparation of RNA, DNA, and protein • High quality RNA and DNA, ready to use for downstream applications • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** • Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping • Reliable interpretation of RNA, DNA, and protein amounts • Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors) • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants • RNA and DNA are ready to use for all typical downstream applications • Proteins are ready to use for SDS-PAGE / Western blotting NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Parallel isolation of RNA and protein from undivided samples • Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required • High RNA yield and integrity • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** Rapid purification of total RNA and protein from cultured cells and tissue • Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening • Broad spectrum of starting material tested and proteins already detected NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Parallel isolation of RNA, DNA, and protein from undivided samples • Convenient one-column preparation of RNA, DNA, and protein • High quality RNA and DNA, ready to use for downstream applications • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins
Technology: Silica-membrane technology Format: Mini spin columns Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL Preparation time 30-35 min/6 preps Applications** • Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping • Reliable interpretation of RNA, DNA, and protein amounts • Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors) • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants • RNA and DNA are ready to use for all typical downstream applications • Proteins are ready to use for SDS-PAGE / Western blotting NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase