PCR产物纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
PCR产物纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
The silica-matrix based kit for PCR clean-up without columns • Batch binding procedure • Quantitative removal of primer and primer-dimer molecules • High recovery for DNA fragments ≥ 120 bp Technology Silica-maxtrix technology Format Silica bead suspension Sample material < 400 µL PCR reaction mixture Fragment size 100 bp–approx. 50 kbp Typical recovery 70–80% A260/A280 1.7–1.9 Elution volume 20–50 µL Preparation time 45 min/6 preps Binding capacity 6 µg/10 µL suspension
NucleoTraP®CR suspension, buffers 10 preps for PCR clean-up - NucleoTraPCR Suspension, buffers pack of 10
The silica-matrix based kit for PCR clean-up without columns • Batch binding procedure • Quantitative removal of primer and primer-dimer molecules • High recovery for DNA fragments ≥ 120 bp Technology Silica-maxtrix technology Format Silica bead suspension Sample material < 400 µL PCR reaction mixture Fragment size 100 bp–approx. 50 kbp Typical recovery 70–80% A260/A280 1.7–1.9 Elution volume 20–50 µL Preparation time 45 min/6 preps Binding capacity 6 µg/10 µL suspension
NucleoTraP®CR suspension, buffers 100 preps for PCR clean-up - NucleoTraPCR Suspension, buffers pack of 100
Magnetic-bead based PCR clean-up • Closed system: reduced risk of cross-contamination • Small elution volumes ≥ 25 μL • Recovery does not depend on elution volume • Easily adapted to automated use
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 30 min/6 strips Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, buffers 12 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, Elution Plate U-bottom, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 45 min/plate Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers 1 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
Magnetic-bead based PCR clean-up • Closed system: reduced risk of cross-contamination • Small elution volumes ≥ 25 μL • Recovery does not depend on elution volume • Easily adapted to automated use
Technology: Magnetic-bead technology Format: Highly reactive superparamagnetic beads Processing: Manual or automated Sample material: < 50 µL PCR reaction mixture Fragment size: 150 bp–10 kbp Typical recovery: 80–95% A260/A280: 1.70–1.90 Elution volume ≥ 25 µL Preparation time: 30–45 min/96 preps Binding capacity: Approx. 0.3 µg/µL beads Applications • Manual or automated PCR clean-up • Typical downstream application: sequencing NucleoMag® P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers 4 x 96 preps for the purification of PCR products - NucleoMag P-Beads, Elution Plates U-bottom, Self-adhering PE Foils, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Cost-efficient 96-well ultrafiltration kit for PCR clean-up • Ready-to-use DNA for sequencing and microarray spotting • Sturdy membrane allows easy recovery of DNA • No well-to-well cross-contamination • Fast procedure • Available as complete kits and as plates only • Detergent-free membrane • Manual or automated use
Technology： Ultrafiltration technology Format： 96-well plates Processing: Manual or automated, vacuum or centrifugation Sample material: 20–300 µL Fragment size: ≥ 150 bp Typical recovery: 40–95% A260/A280: 1.70–1.80 Recovery volume 25–100 µL Preparation time 20 min/plate (for typical PCR reactions of 25 µL) Applications: Purification of PCR products ≥ 150 bp NucleoFast® 96 PCR Plates, Elution Plates U-bottom, Self-adhering Foil, buffers 4 x 96 preps for the purification of PCR products NucleoFast 96 PCR Plates, Elution Plates U-bottom, Self-adhering Foils, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 30 min/6 strips Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Strips, buffers 48 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 45 min/plate Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Plates, buffers 4 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 45 min/plate Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers 4 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, Elution Plates U-bottom, Self-adhering Foils, MN Wash Plates, buffers
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 30 min/6 strips Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, buffers 60 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, MN Wash Plates, Racks of Tube Strips, Elution Plates U-bottom, buffers
Magnetic-bead based PCR clean-up • Closed system: reduced risk of cross-contamination • Small elution volumes ≥ 25 μL • Recovery does not depend on elution volume • Easily adapted to automated use
Technology: Magnetic-bead technology Format: Highly reactive superparamagnetic beads Processing: Manual or automated Sample material: < 50 µL PCR reaction mixture Fragment size: 150 bp–10 kbp Typical recovery: 80–95% A260/A280: 1.70–1.90 Elution volume ≥ 25 µL Preparation time: 30–45 min/96 preps Binding capacity: Approx. 0.3 µg/µL beads Applications • Manual or automated PCR clean-up • Typical downstream application: sequencing NucleoMag® P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers 24 x 96 preps for the purification of PCR products - NucleoMag P-Beads, Elution Plates U-bottom, Self-adhering PE Foils, buffers no dangerous goods according transport regulations
Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput • Time-saving parallel clean-up of PCR products • Complete removal of primers and primer-dimers – optimal recovery • Suitable for processing under vacuum and centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • Suitable for manual or automated use • NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed.
Sample material < 100 µL PCR reaction mixture Fragment size 50 bp–10 kbp Typical recovery 75–95 % A260/A280 1.7–1.8 Elution volume 75–150 µL Preparation time 45 min/plate Binding capacity 15 µg NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers 24 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, MN Wash Plates, Elution Plates U-bottom, Self-adhering Foils, buffers
便宜好用的DNA，dsRNA片段纯化试剂，不需昂贵的硅胶膜纯化柱，也可免去玻璃奶或硅胶珠干燥的困扰，可用于纯化大于75bp的PCR产物，酶切产物，dsRNA，回收率可达98%，产物纯度高，可用于下游应用。可选用粉色沉淀助剂指示沉淀块，不影响下游应用如PCR，克隆和酶反应等。操作简单，只需在样品中加入SureClean Plus，静置10分钟后高速离心沉淀，用70%乙醇洗涤沉淀，最后再次离心沉淀，干燥后即可将沉淀重新溶解于所需的溶液里。a novel, inexpensive solution that provides a column-free method for nucleic acid purification. SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests with excellent recovery rates. SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.
Rapid - column-free PCR clean-up
Efficient - post-PCR recovery up to 98%
Optional pink dye - for improved visibility and minimal pellet loss
Pure - isolated products are suitable for downstream applications
Applications
Removes primers, non-specifics, dNTPs and enzymes
DNA or dsRNA purification or concentration
Buffer exchange
Description
SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.
Using a simple and rapid procedure, SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.
A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns (fig. 1). SureClean Plus purifies nucleic acids without the use of chaotropic salts that often contribute to denaturation of the DNA duplex.
SureClean Plus enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit your downstream experiments.
Optional Pink Co-Precipitant
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids.The co-precipitant does not interfere with downtown applications and so SureClean Plus is suitable for use in all cloning, standard PCR, real-time PCR and other enzymatic reactions.
Notes
Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean solution without pink co-precipitant is recommended.
PCR产物纯化试剂盒，无柱方法，75bp以上片段回收效率98%以上，高效去除引物、dNTPs和酶，适用于DNA或dsRNA的纯化或浓缩；试剂盒中同时含粉红色共沉淀剂，方便对核酸pellets进行可视化监测 便宜好用的DNA，dsRNA片段纯化试剂，不需昂贵的硅胶膜纯化柱，也无需玻璃奶或硅胶珠，可用于纯化大于75bp的PCR产物，酶切产物，dsRNA，回收率可达98%，产物纯度高，可用于下游应用。可选用粉色沉淀助剂指示沉淀块，不影响下游应用如PCR，克隆和酶反应等。操作简单，只需在样品中加入SureClean Plus，静置10分钟后高速离心沉淀，用70%乙醇洗涤沉淀，最后再次离心沉淀，干燥后即可将沉淀重新溶解于所需的溶液里。a novel, inexpensive solution that provides a column-free method for nucleic acid purification. SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests with excellent recovery rates. SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.
Rapid - column-free PCR clean-up
Efficient - post-PCR recovery up to 98%
Optional pink dye - for improved visibility and minimal pellet loss
Pure - isolated products are suitable for downstream applications
Applications
Removes primers, non-specifics, dNTPs and enzymes
DNA or dsRNA purification or concentration
Buffer exchange
Description
SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.
Using a simple and rapid procedure, SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.
A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns (fig. 1). SureClean Plus purifies nucleic acids without the use of chaotropic salts that often contribute to denaturation of the DNA duplex.
SureClean Plus enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit your downstream experiments.
Optional Pink Co-Precipitant
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids.The co-precipitant does not interfere with downtown applications and so SureClean Plus is suitable for use in all cloning, standard PCR, real-time PCR and other enzymatic reactions.
Notes
Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean solution without pink co-precipitant is recommended.
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