FluoroDye DNA Fluorescent Loading Dye (Green,6X), 1 ml x 5 FluoroDye蓝色/紫外光双用倍荧光DNA无毒上样指示染剂,是即用型产品,适用于定性电泳分析,内含高灵敏度荧光染料,对双股DNA具有高度专一性。1mlX5。A ready-to-use 6× DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye DNA Fluorescent Loading Dye allows the user to immediately visualise electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm .
Contents
FluoroDye DNA Fluorescent Loading Dye is stored in 6× concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
FluoroDye DNA Fluorescent Loading Dye (Green,6X), 1 ml FluoroDye蓝色/紫外光双用倍荧光DNA无毒上样指示染剂,是即用型产品,适用于定性电泳分析,内含高灵敏度荧光染料,对双股DNA具有高度专一性。1ml. A ready-to-use 6× DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye DNA Fluorescent Loading Dye allows the user to immediately visualise electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm .
Contents:
FluoroDye DNA Fluorescent Loading Dye is stored in 6× concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
FluoroStain DNA Fluorescent Staining Dye (Green,10,000X), 500 μl FluoroStain蓝光/紫外光双用万倍荧光DNA安全染料,EB替代物,可与双股DNA链结合,经电泳后其检测灵敏度高于EB 10倍以上,检测下限可低至0.1ng。适用于一般紫外光胶体观测仪及较安全的蓝光胶体观测仪的凝胶成像系统。光敏感产品,需避光保存。a safe replacement for the conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for the user.
The FluoroStain DNA Fluorescent Staining Dye offers a safe alternative to EtBr as well as at least 10 times its sensitivity in DNA detection level, capable of detecting double stranded DNA (dsDNA) fragments up to 0.1 ng in electrophoresis analysis.
The FluoroStain DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making destaining process entirely optional.
FluoroStain DNA Fluorescent Staining Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system
SYBR Green I 核酸凝胶染料是目前检测琼脂糖凝胶和聚丙烯酰胺凝胶中双链DNA(dsDNA)和RNA(dsRNA)的更灵敏的染料之一。SYBR Green I 适用于各种电泳分析,使用简单,灵敏度高(在497 nm的透射光下,灵敏度高于EB 染色法10~25 倍),可以检出20pg 的DNA。SYBR Green I 核酸染料与核酸结合后的更大吸收峰在蓝绿可见光区,适用于使用可见光系列仪器对染色结果进行观察。同时,其在紫外区300nm~380nm 也有一个吸收峰,使得其染色结果也可以使用紫外光相关设备进行分析。在300 nm的透射光下,低至60 pg 的dsDNA 条带也能被SYBR Green I 染料检测。这种灵敏度比传统300 nm 透射光下的溴化乙锭(EB)染色至少高了25 倍(图1)。SYBR Green I 染料对于检测寡核苷酸也非常灵敏,能在15%的聚丙烯酰胺凝胶上检测低至1-2 ng 的合成24 聚体,比溴化乙锭的灵敏度高了50-100 倍。同时在不与dsDNA 结合时背景几乎可以忽略。由于SYBR Green I 染料的DNA 结合力强,可将染料加入DNA预先与DNA 包被电泳(电泳前染色)也可在制备凝胶时预先加入SYBR Green I 染料制备凝胶(电泳中染色),以及电泳后染色对DNA 进行染色,此外,SYBR Green I 染料与DNA 的结合不会抑制多种常见的限制性内切酶的活性,包括Hind III 和EcoR I,也不会干扰Southern 杂交分析。同时SYBR Green I染料还可用于DNA 的胶回收实验。详细请联系4008513777.