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生物通商城首页 -> 试剂 > RNA分析 > RNA纯化 > 微量样本RNA纯化

RNA分析

RNAi

miRNA

RNA纯化

RNA保护试剂

RNAse抑制剂

RNase

RNase污染检测

反义RNA

去除环境RNase

RNA合成/体外转录

体外转录+翻译

体外翻译

反转录/cDNA合成

RNA FISH探针/荧光示踪

lncRNA分析

随机引物

Northern Blot

各类细胞(RNAlater保存/提取RNA)

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不限 MNG Bioline
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产品名/货号/品牌 简介 包装  
NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 10 preps

价格:¥

货号:740902.10
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。<5 mg组织, 1-100000个细胞。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
10 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 50 preps

价格:¥

货号:740902.50
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
50 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 250 preps

价格:¥

货号:740902.250
品牌:MNG

经销:基因有限公司

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

Technology: Silica-membrane technology
Format: Mini spin column – XS design
Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
Fragment size: > 200 b
Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9 (depending on sample quality)
Elution volume: 5–30 µL
Preparation time: 40 min/12 preps
Binding capacity: 110 µg

Applications*
• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays
NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
250 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

ISOLATE II RNA 提取试剂盒(微量样本):ISOLATE II RNA Micro Kit 10 preps

价格:¥

货号:BIO-52074
品牌:Bioline

经销:基因有限公司

适合单细胞,显微切割,激光俘获,活检穿刺,流式分选细胞等微量样品的总RNA快速纯化试剂盒(硅胶模离心柱),40分钟可同时完成12个样品操作,载量90ugRNA,洗脱体积5-30ul. for the fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material includes tissue (of up to 5 mg) or small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, cultured cell pellets and flow cytometer sorted cells
•Rapid protocol - 40 min/12 preps
•Perfect for small samples - biopsy or single cells
•High yield and purity - typical A260/A280 ratio 1.9 - 2.1
•Extra filters (shredders) and DNase I included - for excellent RNA recovery

Applications: Isolation of RNA from small amounts of samples:
•Single cells
•Pellets of cultured cells
•Cryosections
•Laser-captured or flow sorted cells
•Small amounts of tissues e.g. biopsy materials
Isolated RNA is ready for downstream applications such as reverse transcription and real-time PCR.

Description
ISOLATE II RNA Micro Kit is specially designed for fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material comprises tissue (up to 5 mg), small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, pellets of cultured cells and flow cytometer sorted cells.

The step-by-step protocol is easy to follow. Cells are lysed with an optimized lysis buffer containing chaotropic ions which simultaneously inactivates RNases, thus preventing degradation of released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit).

There is no need to perform a separate DNase I digestion step. RNA is then bound to the silica membrane. The novel kit design allows elution of RNA in as little as 5–30μl. Simple wash steps remove remaining cell debris. Pure, highly concentrated RNA is eluted in the final step with RNase-free water .

Carrier RNA- poly(-A) RNA is included in the kit, which typically does not interfere with subsequent RT-PCR but allows for optimal performance with the smallest samples.

The isolated RNA shows excellent performance in downstream applications such as reverse transcription, primer extension, Real-Time PCR , Northern blot analysis, microarrays and RNA protection assays.

ISOLATE II RNA 提取试剂盒(微量样本):ISOLATE II RNA Micro Kit 50 preps

价格:¥

货号:BIO-52075
品牌:Bioline

经销:基因有限公司

适合单细胞,显微切割,激光俘获,活检穿刺,流式分选细胞等微量样品的总RNA快速纯化试剂盒(硅胶模离心柱),40分钟可同时完成12个样品操作,载量90ugRNA,洗脱体积5-30ul. for the fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material includes tissue (of up to 5 mg) or small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, cultured cell pellets and flow cytometer sorted cells
•Rapid protocol - 40 min/12 preps
•Perfect for small samples - biopsy or single cells
•High yield and purity - typical A260/A280 ratio 1.9 - 2.1
•Extra filters (shredders) and DNase I included - for excellent RNA recovery

Applications: Isolation of RNA from small amounts of samples:
•Single cells
•Pellets of cultured cells
•Cryosections
•Laser-captured or flow sorted cells
•Small amounts of tissues e.g. biopsy materials
Isolated RNA is ready for downstream applications such as reverse transcription and real-time PCR.

Description
ISOLATE II RNA Micro Kit is specially designed for fast and efficient isolation of extremely pure total RNA from very small samples.

Typical sample material comprises tissue (up to 5 mg), small numbers of cells (up to 5 x 105) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, pellets of cultured cells and flow cytometer sorted cells.

The step-by-step protocol is easy to follow. Cells are lysed with an optimized lysis buffer containing chaotropic ions which simultaneously inactivates RNases, thus preventing degradation of released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit).

There is no need to perform a separate DNase I digestion step. RNA is then bound to the silica membrane. The novel kit design allows elution of RNA in as little as 5–30μl. Simple wash steps remove remaining cell debris. Pure, highly concentrated RNA is eluted in the final step with RNase-free water .

Carrier RNA- poly(-A) RNA is included in the kit, which typically does not interfere with subsequent RT-PCR but allows for optimal performance with the smallest samples.

The isolated RNA shows excellent performance in downstream applications such as reverse transcription, primer extension, Real-Time PCR , Northern blot analysis, microarrays and RNA protection assays.

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