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生物通商城首页 -> 试剂 > RNA分析 > RNA纯化 > 小分子RNA分离

RNA分析

RNAi

miRNA

RNA纯化

RNA保护试剂

RNAse抑制剂

RNase

RNase污染检测

反义RNA

去除环境RNase

RNA合成/体外转录

体外转录+翻译

体外翻译

反转录/cDNA合成

RNA FISH探针/荧光示踪

lncRNA分析

随机引物

Northern Blot

各类细胞(RNAlater保存/提取RNA)

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不限miRNA纯化
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不限 MNG Bioline Biomics
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产品名/货号/品牌 简介 包装  
NucleoSpin 血浆miRNA 提取试剂盒:NucleoSpin miRNA Plasma 10 preps

价格:¥

货号:740981.10
品牌:MNG

经销:基因有限公司

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul,重复上样900ul
Isolation of small RNA and DNA from plasma and serum
• Efficient and selective isolation of small RNA from plasma or serum samples
• Superior recovery of small RNA > 15 nt
• Optional on-column DNA digest for increased sensitivity in downstream applications
• Simple and fast procedure – without phenol / chloroform

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)
Fragment size: < 1000 nt / bp
Elution volume: 20–50 µL
Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)
Binding capacity: 200 µg

Applications**
• Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)
• Typical downstream applications: real-time qRT-PCR, chip hybridisations
NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase
10 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin 血浆miRNA 提取试剂盒:NucleoSpin miRNA Plasma 50 preps

价格:¥

货号:740981.50
品牌:MNG

经销:基因有限公司

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul,重复上样900ul
Isolation of small RNA and DNA from plasma and serum
• Efficient and selective isolation of small RNA from plasma or serum samples
• Superior recovery of small RNA > 15 nt
• Optional on-column DNA digest for increased sensitivity in downstream applications
• Simple and fast procedure – without phenol / chloroform

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)
Fragment size: < 1000 nt / bp
Elution volume: 20–50 µL
Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)
Binding capacity: 200 µg

Applications**
• Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)
• Typical downstream applications: real-time qRT-PCR, chip hybridisations
NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase
50 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin miRNA 提取试剂盒:NucleoSpin miRNA 50 preps

价格:¥

货号:740971.50
品牌:MNG

经销:基因有限公司

方便快速地提取< 200 b的小分子RNA,离心柱式多用途试剂盒也可以分别提取小分子RNA和大分子RNA,以及蛋白质组分。出色的小分子RNA回收率,无需酚氯仿,提供NucleoSpin® Filters有效降低裂解液粘稠度便于均质化,提供rDNase去除DNA污染
Parallel isolation of small and large RNA
• RNA purification fractionated by size: Isolation of small RNA only (< 200 b),
Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
• NucleoSpin® Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 10(7) cultured cells, < 30 mg human / animal tissue; < 50 mg plant tissue, < 150 µL reaction mixture
Fragment size: Small RNA: < 200 b, large RNA: > 200 b
Typical yield: 10 µg small RNA, 95 µg large RNA from 10(7) HeLa cells
Elution volume: 30–100 µL
Preparation time: < 45 min (6 preps human / animal tissue, small and large RNA)
< 35 min (6 preps human / animal tissue, small RNA only)
Binding capacity 200 µg

Applications**
• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol®) lysis
• Purification of siRNA and large dsRNA from DICER reactionsTypical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization. Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
NucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of small and large RNA - NucleoSpin RNA Columns, NucleoSpin miRNA Columns, NucleoSpin Filters, NucleoSpin Protein Removal Columns, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin 血浆miRNA 提取试剂盒:NucleoSpin miRNA Plasma 250 preps

价格:¥

货号:740981.250
品牌:MNG

经销:基因有限公司

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul,重复上样900ul
Isolation of small RNA and DNA from plasma and serum
• Efficient and selective isolation of small RNA from plasma or serum samples
• Superior recovery of small RNA > 15 nt
• Optional on-column DNA digest for increased sensitivity in downstream applications
• Simple and fast procedure – without phenol / chloroform

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)
Fragment size: < 1000 nt / bp
Elution volume: 20–50 µL
Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)
Binding capacity: 200 µg

Applications**
• Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)
• Typical downstream applications: real-time qRT-PCR, chip hybridisations
NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase
250 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin miRNA 提取试剂盒:NucleoSpin miRNA 250 preps

价格:¥

货号:740971.250
品牌:MNG

经销:基因有限公司

方便快速地提取< 200 b的小分子RNA,离心柱式多用途试剂盒也可以分别提取小分子RNA和大分子RNA,以及蛋白质组分。出色的小分子RNA回收率,无需酚氯仿,提供NucleoSpin® Filters有效降低裂解液粘稠度便于均质化,提供rDNase去除DNA污染
Parallel isolation of small and large RNA
• RNA purification fractionated by size: Isolation of small RNA only (< 200 b),
Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
• NucleoSpin® Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 10(7) cultured cells, < 30 mg human / animal tissue; < 50 mg plant tissue, < 150 µL reaction mixture
Fragment size: Small RNA: < 200 b, large RNA: > 200 b
Typical yield: 10 µg small RNA, 95 µg large RNA from 10(7) HeLa cells
Elution volume: 30–100 µL
Preparation time: < 45 min (6 preps human / animal tissue, small and large RNA)
< 35 min (6 preps human / animal tissue, small RNA only)
Binding capacity 200 µg

Applications**
• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol®) lysis
• Purification of siRNA and large dsRNA from DICER reactionsTypical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization. Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
NucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the isolation of small and large RNA - NucleoSpin RNA Columns, NucleoSpin miRNA Columns, NucleoSpin Filters, NucleoSpin Protein Removal Columns, Collection Tubes, buffers, RNase-free rDNase

ISOLATE II miRNA 提取试剂盒:ISOLATE II miRNA Kit 25 preps

价格:¥

货号:BIO-52083
品牌:Bioline

经销:基因有限公司

从细胞/组织中快速,无偏向性分离富集小分子RNA(包括miRNA, siRNA和small RNA <200nt),操作非常简便,不需酚,裂解后第一次离心过柱去除大分子RNA(可选择同时纯化大分子RNA),第二次离心过柱吸附纯化小分子RNA。包含DNAseI,纯化柱最高载量50ug,洗脱体积最小20ul。for the rapid and unbiased, phenol-free isolation of highly enriched small RNA( <200nt ) with superior yield and purity, from a wide range of cells and tissues. Isolate high quality large RNA >200nt in parallel from the same sample. Ideal for highly sensitive applications...

•Rapid, phenol-free workflow - unbiased small RNA isolation
•Novel separation matrix - highest yields and purity
•Highly specific small RNA enrichment - highest recovery of miRNA, siRNA and all other small RNA <200nt
•Parallel large RNA purification (optional) - high quality large RNA >200nt
•DNase I included - highly efficient digestion of genomic DNA

Applications Isolation of miRNA and other small RNA from
•Mammalian tissue and cell lines
•Bacteria and yeast
•Biofluids (saliva, semen, urine, blood, plasma, serum)
Fully compatible with highly sensitive applications such as real-time RT-PCR, microarrays, single-cell assays and next-generation RNA sequencing (small RNA-seq).

Description
ISOLATE II miRNA Kit is specially developed for the rapid, phenol-free isolation of extremely pure and highly enriched small RNA (<200nt) including miRNA, siRNA, snRNA and piRNA, without bias . High quality large RNA (>200nt) such as mRNA can be easily sequentially purified from the same sample, as a separate fraction .

ISOLATE II miRNA Kit is optimized to deliver superior recovery from a wide range of sample sources such as mammalian tissue, cell lines, bacteria, yeast, blood and other biofluids, including challenging samples with low RNA content.

ISOLATE II miRNA Kit allows convenient processing of multiple samples in 25 minutes, without toxic chemicals such as phenol and chloroform. Isolation is based on fast spin column technology using a new generation silica. The novel separation matrix preferentially purifies small RNA from other cellular components and contaminants without laborious phenol-based phase separation and alcohol precipitation steps. In contrast, phenol-carryover is observed in preparations of small RNA using phenol-based methods and can affect performance in sensitive downstream applications.

Moreover, phenol-chloroform isolation has been reported to introduce significant bias in recovery of selected small RNA, resulting in unreliable results. ISOLATE II miRNA Kit has been developed to overcome the bias observed with small RNA isolation using phenol-based techniques, due to its proprietary small RNA separation and enrichment technology, as well as highly optimized chemistry. ISOLATE II miRNA Kit utilizes a two-column system to enrich exclusively for small RNAs, with complete diversity and no background of large RNA. Large RNA greater than 200 nucleotides are bound to the first column and ethanol is then added to the flow-through in order to increase the binding affinity of small RNA to the second column. Once captured and enriched onto the second column, small RNA molecules are then eluted, ready-for-use.

The highly purified and enriched small RNA fraction can be used for the most sensitive applications including miRNA expression profiling and quantification using real-time PCR, RT-PCR, microarrays, small RNA library construction for small RNA-seq, as well as single cell assays.

EzOmics™ RNA QUICK clear KIT--一步纯化RNA转录产物 请咨询

价格:¥

货号:RNA KIT
品牌:Biomics

经销:百奥迈科

可和Biomics生产的 T7 sgRNA MICscript™ KIT配套使用。使用该试剂盒纯化后的sgRNA可直接进行转染及细胞显微注射。详细请联系4008513777.

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