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生物通商城首页 -> 试剂 -> RNA分析 -> 反转录/cDNA合成

RNA分析

RNAi

miRNA

RNA纯化

RNA保护试剂

RNAse抑制剂

RNase

RNase污染检测

反义RNA

去除环境RNase

RNA合成/体外转录

体外转录+翻译

体外翻译

反转录/cDNA合成

RNA FISH探针/荧光示踪

lncRNA分析

随机引物

Northern Blot

各类细胞(RNAlater保存/提取RNA)

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产品名/货号/品牌 简介 包装  
M-MuLV 反转录酶:M-MuLV Reverse Transcriptase 5,000 units

价格:¥

货号:M0253V
品牌:NEB

经销:基因有限公司

一种RNA介导的DNA聚合酶。该酶能以RNA(合成 cDNA 时)或单链DNA做模板由引物起始合成一条互补的 DNA。M-MuLV 反转录酶无3→5´ 核酸外切酶活性。200,000 units/ml

AMV 反转录酶:AMV Reverse Transcriptase 200 units

价格:¥

货号:M0277S
品牌:NEB

经销:基因有限公司

禽骨髓母细胞瘤病毒(AMV )反转录酶是一种RNA介导的DNA聚合酶。该酶能以RNA(合成cDNA 时)或单链DNA为模板从引物开始合成互补的DNA链。10,000 units/ml。

M-MuLV 反转录酶:M-MuLV Reverse Transcriptase 10,000 units

价格:¥

货号:M0253S
品牌:NEB

经销:基因有限公司

一种RNA介导的DNA聚合酶。该酶能以RNA(合成 cDNA 时)或单链DNA做模板由引物起始合成一条互补的 DNA。M-MuLV 反转录酶无3→5´ 核酸外切酶活性。200,000 units/ml

ProtoScript® II 反转录酶 :ProtoScript II Reverse Transcriptase 4,000 units

价格:¥

货号:M0368S
品牌:NEB

经销:基因有限公司

ProtoScript Ⅱ反转录酶是M-MuLV反转录酶的重组蛋白,其RNase H活性降低且热稳定性增加。与野生型 M-MuLV 反转录酶相比,ProtoScript Ⅱ可在更高的温度下合成第一链cDNA。ProtoScript Ⅱ活性温度高达50℃,且具有特异性高、产量高和合成 cDNA 更长的特点,合成cDNA长度可达12 kb。

WarmStar RTx 反转录酶:WarmStart RTx Reverse Transcriptase 50 rxns

价格:¥

货号:M0380S
品牌:NEB

经销:基因有限公司

WarmStart® RTx Reverse Transcriptase, 50 reactions 热启动反转录酶,能够以RNA或单链DNA为模板合成双链DNA,15,000units/ml,共50个反应,非常适用于环介导等温扩增。

AMV 反转录酶:AMV Reverse Transcriptase 高浓度,500 units

价格:¥

货号:M0277T
品牌:NEB

经销:基因有限公司

禽骨髓母细胞瘤病毒(AMV )反转录酶是一种RNA介导的DNA聚合酶。该酶能以RNA(合成cDNA 时)或单链DNA为模板从引物开始合成互补的DNA链。25,000 units/ml。

AMV cDNA 第一链合成试剂盒:AMV First Strand cDNA Synthesis Kit 30 rxns

价格:¥

货号:E6550S
品牌:NEB

经销:基因有限公司

AMV cDNA 第一链合成试剂盒含有 AMV 酶混合液与 AMV 2X 反应混合液。AMV 酶混合液是 AMV 反转录酶与小鼠 RNase 抑制剂的优化混合。使用 AMV 反转录酶合成 cDNA 第一链时,最佳反应温度范围广,可以从 37℃ 到 50℃。小鼠 RNase 抑制剂比人 RNase 抑制剂更耐氧化,因而能更好地保护 RNA 模板。AMV 2X 反应混合液是含有 dNTPs 的优化缓冲液。在 1X AMV 反应混合液中,AMV 酶混合液能够以高产量合成长 cDNA(达 10
AMV酶混合液是AMV反转录酶与小鼠RNase抑制剂的优化混合。最佳反应温度范围广,可以从 37℃ 到50℃。小鼠 RNase 抑制剂比人 RNase 抑制剂更耐氧化,因而能更好地保护 RNA 模板。AMV 2X 反应混合液是含有 dNTPs 的优化缓冲液。AMV 酶混合液能够以高产量合成10 kb的长cDNA。含10X AMV 酶混合液,AMV 2X反应缓冲混合液,随机引物混合液(60 μM)、Oligo d(T)23VN 引物 (50 μM)、无核酸酶污染的水

ProtoScript® II 反转录酶 :ProtoScript II Reverse Transcriptase 10,000 units

价格:¥

货号:M0368L
品牌:NEB

经销:基因有限公司

ProtoScript Ⅱ反转录酶是M-MuLV反转录酶的重组蛋白,其RNase H活性降低且热稳定性增加。与野生型 M-MuLV 反转录酶相比,ProtoScript Ⅱ可在更高的温度下合成第一链cDNA。ProtoScript Ⅱ活性温度高达50℃,且具有特异性高、产量高和合成 cDNA 更长的特点,合成cDNA长度可达12 kb。

AMV 反转录酶:AMV Reverse Transcriptase 1,000 units

价格:¥

货号:M0277L
品牌:NEB

经销:基因有限公司

禽骨髓母细胞瘤病毒(AMV )反转录酶是一种RNA介导的DNA聚合酶。该酶能以RNA(合成cDNA 时)或单链DNA为模板从引物开始合成互补的DNA链。10,000 units/ml。

M-MuLV 反转录酶:M-MuLV Reverse Transcriptase 50,000 units

价格:¥

货号:M0253L
品牌:NEB

经销:基因有限公司

一种RNA介导的DNA聚合酶。该酶能以RNA(合成 cDNA 时)或单链DNA做模板由引物起始合成一条互补的 DNA。M-MuLV 反转录酶无3→5´ 核酸外切酶活性。200,000 units/ml

WarmStar RTx 反转录酶:WarmStart RTx Reverse Transcriptase 250 rxns

价格:¥

货号:M0380L
品牌:NEB

经销:基因有限公司

WarmStart® RTx Reverse Transcriptase, 250 reactions 热启动反转录酶,能够以RNA或单链DNA为模板合成双链DNA,15,000units/ml,共250个反应,非常适用于环介导等温扩增。

ProtoScript® II 反转录酶 :ProtoScript II Reverse Transcriptase 400,000 units

价格:¥

货号:M0368X
品牌:NEB

经销:基因有限公司

ProtoScript Ⅱ反转录酶是M-MuLV反转录酶的重组蛋白,其RNase H活性降低且热稳定性增加。与野生型 M-MuLV 反转录酶相比,ProtoScript Ⅱ可在更高的温度下合成第一链cDNA。ProtoScript Ⅱ活性温度高达50℃,且具有特异性高、产量高和合成 cDNA 更长的特点,合成cDNA长度可达12 kb。

Reverse Transcriptase, M-MuLV:Reverse Transcriptase, M-MuLV 10000 U

价格:¥

货号:sc-43500
品牌:Santa Cruz

经销:基因有限公司

10,000 URecombinant enzyme possessing full reverse transcriptase and RNase H activitiesSupplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25° C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT)Suitable for synthesis of full-length first strand cDNA up to 13 kbOptimum activity at 42° CActive up to 50° C

Tetro cDNA合成试剂盒:Tetro cDNA Synthesis Kit 30 Reactions

价格:¥

货号:BIO-65042
品牌:Bioline

经销:基因有限公司

cDNA合成试剂盒(30次),将RNA逆转录成cDNA,试剂盒中含酶、buffer、随机引物、Oligo dT引物、dNTP等反应所需组分。起始模板量可以低至10pg总RNA,适用于长模板和稀有模板的逆转录,模板范围10pg-2ug cDNA合成试剂盒,灵敏度高适用于从10pg到2ug的启始RNA,长基因或者低丰度模板均可胜任。 Contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable reverse transcriptase -for long genes and rare transcripts
Simple -one-tube, one-step set-up for highly sensitive PCR
Broad dynamic range -10pg to 2μg of RNA

Applications
Construction of cDNA libraries
2-step PCR assays
Generation of probes for hybridization
Gene cloning

Description
The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA.

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

SensiFAST cDNA快速合成试剂盒:SensiFAST cDNA Synthesis Kit 50 Reactions

价格:¥

货号:BIO-65053
品牌:Bioline

经销:基因有限公司

cDNA快速合成试剂盒(50次),将RNA逆转录成cDNA,试剂盒中含逆转录酶、TransAmp buffer等反应所需组分,配制反应体系更方便快捷,减少污染风险。起始模板量可以低至1pg总RNA,适用于低拷贝模板扩增,RNA全长均一扩增性能卓越 第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
Broad dynamic range – ideal for dilute and low-copy samples
Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
Applications
Two-step RT-PCR
Two-step RT-qPCR

Description
SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

Tetro 逆转录酶:Tetro Reverse Transcriptase Enzyme 10,000 Units

价格:¥

货号:BIO-65050
品牌:Bioline

经销:基因有限公司

逆转录酶(10000u),将RNA你转录成cDNA的酶,产品包括逆转录酶和配套buffer。具有高灵敏性、产量高、模板范围广等特点 MMLv,灵敏度高,可从10pg总RNA中合成高质量全长cDNA。最高可耐受48度以减少RNA二级结构。 A highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.

Highly sensitive - for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable - for long genes and rare transcripts
High yield - produces high quality cDNA ideal for PCR
Broad dynamic range - 10pg to 2μg of RNA
Description
Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor , with highly efficient and sensitive transcription, from as little as 10pg, up to 2μg of RNA .

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

Tetro cDNA合成试剂盒:Tetro cDNA Synthesis Kit 100 Reactions

价格:¥

货号:BIO-65043
品牌:Bioline

经销:基因有限公司

cDNA合成试剂盒(100次),将RNA逆转录成cDNA,试剂盒中含酶、buffer、随机引物、Oligo dT引物、dNTP等反应所需组分。起始模板量可以低至10pg总RNA,适用于长模板和稀有模板的逆转录,模板范围10pg-2ug cDNA合成试剂盒,灵敏度高适用于从10pg到2ug的启始RNA,长基因或者低丰度模板均可胜任。 Contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable reverse transcriptase -for long genes and rare transcripts
Simple -one-tube, one-step set-up for highly sensitive PCR
Broad dynamic range -10pg to 2μg of RNA

Applications
Construction of cDNA libraries
2-step PCR assays
Generation of probes for hybridization
Gene cloning

Description
The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA.

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

Tetro 逆转录酶:Tetro Reverse Transcriptase Enzyme 4 x 10,000 Units

价格:¥

货号:BIO-65051
品牌:Bioline

经销:基因有限公司

逆转录酶(4x10000u),将RNA你转录成cDNA的酶,产品包括逆转录酶和配套buffer。具有高灵敏性、产量高、模板范围广等特点 MMLv,灵敏度高,可从10pg总RNA中合成高质量全长cDNA。最高可耐受48度以减少RNA二级结构。 A highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.

Highly sensitive - for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable - for long genes and rare transcripts
High yield - produces high quality cDNA ideal for PCR
Broad dynamic range - 10pg to 2μg of RNA
Description
Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor , with highly efficient and sensitive transcription, from as little as 10pg, up to 2μg of RNA .

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

SensiFAST cDNA快速合成试剂盒:SensiFAST cDNA Synthesis Kit 250 Reactions

价格:¥

货号:BIO-65054
品牌:Bioline

经销:基因有限公司

cDNA快速合成试剂盒(250次),将RNA逆转录成cDNA,试剂盒中含逆转录酶、TransAmp buffer等反应所需组分,配制反应体系更方便快捷,减少污染风险。起始模板量可以低至1pg总RNA,适用于低拷贝模板扩增,RNA全长均一扩增性能卓越 第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
Broad dynamic range – ideal for dilute and low-copy samples
Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
Applications
Two-step RT-PCR
Two-step RT-qPCR

Description
SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

逆转录酶:Reverse Transcriptase 10,000 Units x 2

价格:¥

货号:RP1000
品牌:SMOBIO

经销:基因有限公司

ExcelRT Reverse Transcriptase, 10,000 Units x 2 ExcelRT高效逆转录酶,莫罗尼鼠白血病病毒逆转录酶改造而成,可利用RNA合成cDNA,具有RNase H活性低,热稳定性强,可合成大于8kbcDNA等特点。200 U/ µl, 10,000 U × 2. Recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, a RNA dependent DNA polymerase capable of generating the first strand cDNA using RNA template. Features • High yield • Thermostable, up to 50°C, during first strand synthesis • High processivity, generating cDNA up to 8 kb • Reduced RNase H ribonuclease activity • No detectable a 3’→ 5’ exonucleolytic proofreading function • Thermal stable for at least 4 weeks when stored at 4°C

逆转录试剂盒:Reverse Transcription kit 100 Rxn

价格:¥

货号:RP1300
品牌:SMOBIO

经销:基因有限公司

逆转录试剂盒 II:Reverse Transcription kit 100 Rxn

价格:¥

货号:RP1400
品牌:SMOBIO

经销:基因有限公司

HiScript® Reverse Transcriptase 2000 U

价格:¥

货号:R101-01
品牌:Vazyme

经销:南京诺唯赞生物

HiScript® Reverse Transcriptase是在M-MLV (RNase H-) Reverse Transcriptase基础上经过多点突变得到的全新逆转录酶。HiScript® Reverse Transcriptase在50℃下活性最高,且可耐受高达55℃的反应温度,可用于具有二级结构的RNA模板的逆转录。增强与模板的亲和力,可获得更多全长的cDNA,并可检测到低至1 pg的总RNA,特别适合少量模板以及低拷贝基因的逆转录。此外HiScript® Reverse Transcrip

HiScript® II Reverse Transcriptase 2000U

价格:¥

货号:R201-01
品牌:Vazyme

经销:南京诺唯赞生物

产品描述在上一代的HiScript® Reverse Transcriptase的基础上,我们通过专利的体外分子进化技术,获得了一个全新的逆转录酶突变体-HiScript® II Reverse Transcriptase。其携带的多个点突变使得其热稳定性大幅提高,在50℃的半衰期超过240分钟,并可在55℃长时间反应而保持稳定,保证了具有复杂二级结构模板的逆转录成功率。同时,高温下保持的高活性能够带来更高的cDNA产量。同时,HiScript® II Reverse Transcriptase与模板的亲

M-MLV Reverse Transcriptase 10000 U

价格:¥

货号:R011-01
品牌:Vazyme

经销:南京诺唯赞生物

野生型M-MLV (Moloney Murine Leukemia Virus)具有以下几种活性:依赖于RNA的DNA聚合酶活性;依赖于DNA的DNA聚合酶活性;RNase H活性。由于RNase H活性能够催化降解DNA/RNA杂合体中的RNA,因此在cDNA第一条链的合成反应中可能会降解模板RNA。M-MLV (H-) Reverse Transcriptase是通过定点突变得到的RNase H活性缺失的M-MLV突变体。与常见的通过删除(deletion) RNase H结构域方法得到的突变体相比,

M-MLV(H-) Reverse Transcriptase 10000 U

价格:¥

货号:R021-01
品牌:Vazyme

经销:南京诺唯赞生物

野生型M-MLV (Moloney Murine Leukemia Virus)具有以下几种活性:依赖于RNA的DNA聚合酶活性;依赖于DNA的DNA聚合酶活性;RNase H活性。由于RNase H活性能够催化降解DNA/RNA杂合体中的RNA,因此在cDNA第一条链的合成反应中可能会降解模板RNA。M-MLV (H-) Reverse Transcriptase是通过定点突变得到的RNase H活性缺失的M-MLV突变体。与常见的通过删除(deletion) RNase H结构域方法得到的突变体相比,

HiScript® Reverse Transcriptase 10000 U

价格:¥

货号:R101-02
品牌:Vazyme

经销:南京诺唯赞生物

HiScript® Reverse Transcriptase是在M-MLV (RNase H-) Reverse Transcriptase基础上经过多点突变得到的全新逆转录酶。HiScript® Reverse Transcriptase在50℃下活性最高,且可耐受高达55℃的反应温度,可用于具有二级结构的RNA模板的逆转录。增强与模板的亲和力,可获得更多全长的cDNA,并可检测到低至1 pg的总RNA,特别适合少量模板以及低拷贝基因的逆转录。此外HiScript® Reverse Transcrip

HiScript® 1st Strand cDNA Synthesis Kit 50 rxn (20 μl/rxn)

价格:¥

货号:R111-01
品牌:Vazyme

经销:南京诺唯赞生物

HiScript® 第一链cDNA合成试剂盒基于HiScript® Reverse Transcriptase,包含合成高质量第一链cDNA所需的所有成分,适合于后续的PCR、qPCR以及PCR克隆。2 × RT Mix包含优化的缓冲体系和dNTP;Enzyme Mix包含HiScript® Reverse Transcriptase和RNase inhibitor。可根据需要,选择Oligo (dT)18或Random hexamers作为逆转录引物。

HiScript® II 1st Strand cDNA Synthesis Kit 50 rxn (20 μl/rxn)

价格:¥

货号:R211-01
品牌:Vazyme

经销:南京诺唯赞生物

HiScript® II两步法RT-PCR/qPCR系列试剂盒以高性能的HiScript® II Reverse Transcriptase为基础,对反应体系进行了大幅简化,特别是即用型的SuperMix系列产品,将合成高质量第一链cDNA所需的所有成分合并为一管5 × Mix,只需加入RNA和水即可进行逆转录反应,且RNA体积最多可加至反应总体积的80%,非常适合于低浓度RNA模板的逆转录反应,且SuperMix在-20℃不会冻结,使用方便。RNA模板中的基因组污染往往会对后续的定量PCR结果造成很大干

HiScript® II Reverse Transcriptase 10000U

价格:¥

货号:R201-02
品牌:Vazyme

经销:南京诺唯赞生物

产品描述在上一代的HiScript® Reverse Transcriptase的基础上,我们通过专利的体外分子进化技术,获得了一个全新的逆转录酶突变体-HiScript® II Reverse Transcriptase。其携带的多个点突变使得其热稳定性大幅提高,在50℃的半衰期超过240分钟,并可在55℃长时间反应而保持稳定,保证了具有复杂二级结构模板的逆转录成功率。同时,高温下保持的高活性能够带来更高的cDNA产量。同时,HiScript® II Reverse Transcriptase与模板的亲

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