识别位点:G/GATCC
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:-
热灭活温度:-
稀释液:A 
粘端匹配的酶:BclI, DpnII；BglII, BstYI (R/GATCY)，BstYI (G/GATCC)
同裂酶:-

识别位点:G/GATCC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:-
稀释液:A 
粘端匹配的酶:BclI, DpnII；BglII, BstYI (R/GATCY)，BstYI (G/GATCC)
同裂酶:-

识别位点:G/AATTC
缓冲液:NEBuffer EcoRI
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping
热灭活温度:65°C
稀释液:C 
粘端匹配的酶:-
同裂酶:FunII

识别位点:G/AATTC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping
热灭活温度:65°C
稀释液:C 
粘端匹配的酶:-
同裂酶:-

识别位点:A/AGCTT
缓冲液:NEBuffer 2.1
反应温度:37°C
甲基化敏感性:-
热灭活温度:80°C
稀释液:B 
粘端匹配的酶:-
同裂酶:-

识别位点:A/AGCTT
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:80°C
稀释液:B 
粘端匹配的酶:-
同裂酶:-

识别位点:T/GATCA
缓冲液:NEBuffer 3.1
反应温度:50°C
甲基化敏感性:dam methylation: Blocked
热灭活温度:-
稀释液:A 
粘端匹配的酶:BamHI, BstYI (R/GATCY)；BglII, MboII
同裂酶:BsiQI, FbaI, Ksp22I

识别位点:GCCNNNN/NGGC
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping
热灭活温度:65°C
稀释液:B 
粘端匹配的酶:-
同裂酶:-

识别位点:A/GATCT
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:-
热灭活温度:-
稀释液:A 
粘端匹配的酶:BamHI, BclI, DpnII，BstYI (R/GATCY)
同裂酶:-

识别位点:CC/WGG
缓冲液:NEBuffer 3.1
反应温度:60°C
甲基化敏感性:-
热灭活温度:-
稀释液:A 
粘端匹配的酶:-
同裂酶:AjnI ^, BciT130I, BptI, BseBI, BsiLI, Bst2UI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^

识别位点:GAG/CTC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping
热灭活温度:65°C
稀释液:A 
粘端匹配的酶:-
同裂酶:Ecl136II, EcoICRI, Psp124BI ^, SacI^, SstI^

识别位点:GAT/ATC
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:CpG Methylation: Impaired by Some Combinations of Overlapping
热灭活温度:80°C
稀释液:A 
粘端匹配的酶:-
同裂酶:Eco32I

识别位点:GAT/ATC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Impaired by Some Combinations of Overlapping
热灭活温度:65°C
稀释液:B 
粘端匹配的酶:-
同裂酶:Eco32I

识别位点:GCG/C
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked
热灭活温度:65°C
稀释液:A 
粘端匹配的酶:-
同裂酶:AspLEI , BstHHI, CfoI, GlaI^, R9529^, HinP1I^, HspAI ^

识别位点:TCG/CGA
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:dam methylation: Blocked by Overlapping; CpG Methylation: Blocked
热灭活温度:-
稀释液:A 
粘端匹配的酶:-
同裂酶:Bsp68I, BtuMI, RruI^

识别位点:C/TCGAG
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked
热灭活温度:-
稀释液:A 
粘端匹配的酶:-
同裂酶:BssHI, Sfr274I, SlaI, StrI, TliI, XhoI

识别位点:CAG/CTG
缓冲液:NEBuffer 3.1
反应温度:37°C
甲基化敏感性:-
热灭活温度:-
稀释液:B 
粘端匹配的酶:-
同裂酶:-

识别位点:CAG/CTG
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:-
稀释液:B 
粘端匹配的酶:-
同裂酶:-

识别位点:GT/AC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping
热灭活温度:-
稀释液:A 
粘端匹配的酶:-
同裂酶:AfaI , Csp6I^, CviQI^

识别位点:GAGCT/C
缓冲液:NEBuffer 1.1
反应温度:37°C
甲基化敏感性:-
热灭活温度:65°C
稀释液:A 
粘端匹配的酶:BanII (GAGCT/C), BsiHKAI, Bsp1286I (GAGCT/C)
同裂酶:Ecl136II^, Eco53kI^, EcoICRI^, Psp124BI , SstI

识别位点:GAGCT/C
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:65°C
稀释液:A 
粘端匹配的酶:BanII (GAGCT/C), BsiHKAI, Bsp1286I (GAGCT/C)
同裂酶:Ecl136II^, Eco53kI^, EcoICRI^, Psp124BI , SstI

识别位点:TAC/GTA
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked
热灭活温度:80°C
稀释液:A 
粘端匹配的酶:-
同裂酶:BstSNI, Eco105I

识别位点:GACN/NNGTC
缓冲液:CutSmart Buffer
反应温度:65°C
甲基化敏感性:-
热灭活温度:-
稀释液:B 
粘端匹配的酶:-
同裂酶:AspI , PflFI, PsyI, TelI

识别位点:GACGT/C
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:80°C
稀释液:B 
粘端匹配的酶:-
同裂酶:ZraI^

识别位点:C/YCGRG
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:CpG Methylation: Blocked
热灭活温度:80°C
稀释液:A 
粘端匹配的酶:AvaI(C/CCGGG)-AgeI, BsaWI, BspEI, BsrFI (R/CCGGY), NgoMIV, SgrAI (CR/CCGGYG)； AvaI(C/TCGAG)-XhoI，SalI； AvaI(C/CCGGG)-XmaI
同裂酶:Ama87I , BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII

识别位点:G/GWCC
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:dcm & CpG Methylation: Blocked by Overlapping
热灭活温度:80°C
稀释液:A 
粘端匹配的酶:PpuMI (RG/GACCY，RG/GTCCY)，RsrII
同裂酶:AflI , Bme18I, Eco47I, PspO3I^, SinI, Vpak11AI^

识别位点:G/GTNACC
缓冲液:NEBuffer 3.1
反应温度:60°C
甲基化敏感性:-
热灭活温度:-
稀释液:A 
粘端匹配的酶:-
同裂酶:BstPI, Eco065I, Eco91I, EcoO65I, PspEI

CutSmart、重组酶、基因工程改造酶、省时酶、高保真酶。对 dam、dcm 和哺乳动物 CpG 甲基化均不敏感。 High-Fidelity (HF®) Restriction Endonucleases, Restriction Endonucleases: B, Time-Saver™ Qualified Restriction Enzymes；High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

识别位点:C/TNAG
缓冲液:CutSmart Buffer
反应温度:37°C
甲基化敏感性:-
热灭活温度:65°C
稀释液:B 
粘端匹配的酶:-
同裂酶:BstDEI, HpyF3I