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特性:
不限热启动高保真聚合酶热启动高保真聚合酶预混液热启动聚合酶热启动酶预混液
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不限 NEB USB Vazyme ENZYMATICS INC. Bioline
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产品名/货号/品牌 简介 包装  
热启动 Taq DNA 聚合酶:Hot Start Taq DNA Polymerase 200 units

价格:¥

货号:M0495S
品牌:NEB

经销:基因有限公司

常规热启动Taq酶,延伸速度 1 kb/min;扩增长度 ≤ 5 kb;1.25 Unit/50ul反应体系;3'A末端。与化学修饰或基于抗体结合的热启动聚合酶不同,NEB 的热启动 Taq 利用一种基于核酸适配体(aptamer)技术。这种独特的核酸适配体通过非共价键作用与聚合酶结合,从而抑制聚合酶在非许可温度下的聚合反应。这种新方法不需要激活步骤,减少样品降解的可能性、缩短整个反应时间。

OneTaq® 热启动 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start 2X Master Mix with GC Buffer 100 rxns

价格:¥

货号:M0485S
品牌:NEB

经销:基因有限公司

High GC Enhancer 随 GC 缓冲液一同提供。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体

OneTaq® 热启动 2X Master Mix(提供标准缓冲液):OneTaq Hot Start 2X Master Mix with Standard Buffer 100 rxns

价格:¥

货号:M0484S
品牌:NEB

经销:基因有限公司

OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二

OneTaq® 热启动 Quick-Load 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer 100 rxns

价格:¥

货号:M0489S
品牌:NEB

经销:基因有限公司

Quick-Load形式酶预混液,可用于直接凝胶上样。High GC Enhancer 随 GC 缓冲液一同提供。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的

OneTaq® 热启动 Quick-Load® 2X Master Mix(提供标准缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer 100 rxns

价格:¥

货号:M0488S
品牌:NEB

经销:基因有限公司

Quick-Load形式酶预混液,可用于直接凝胶上样。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体(a

热启动 Taq 2X Master Mix:Hot Start Taq 2X Master Mix 100 rxns

价格:¥

货号:M0496S
品牌:NEB

经销:基因有限公司

常规热启动Taq酶预混液,延伸速度 1 kb/min;扩增长度 ≤ 5 kb;1.25 Unit/50ul反应体系;3'A末端。与化学修饰或基于抗体结合的热启动聚合酶不同,NEB 的热启动 Taq 利用一种基于核酸适配体(aptamer)技术。这种独特的核酸适配体通过非共价键作用与聚合酶结合,从而抑制聚合酶在非许可温度下的聚合反应。这种新方法不需要激活步骤,减少样品降解的可能性、缩短整个反应时间。

OneTaq® 热启动 DNA 聚合酶:OneTaq Hot Start DNA Polymerase 200 units

价格:¥

货号:M0481S
品牌:NEB

经销:基因有限公司

延伸速率1kb/min. 扩增长度≤6kb。保真度〉2X Taq; 1.25units/50ul反应体系。 产物末端结构:3'A。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二聚体和

Q5® 热启动超保真 DNA 聚合酶:Q5 Hot Start High-Fidelity DNA Polymerase 100 units

价格:¥

货号:M0493S
品牌:NEB

经销:基因有限公司

Q5 热启动超保真DNA聚合酶延伸速度 6kb/min;扩增长度 ≤ 20kb;高保真度:>100X Taq;1 Unit/50ul反应体系;平末端。Q5 无与伦比的扩增保真性(> Taq 酶的100倍)及其超低的错配率,建立了高保真聚合酶的新标准。无论延伸速度,扩增长度,保真度和可靠性都远远超过常规Taq酶。NEB的 Q5 采用了独特的核酸适配体修饰的热启动方式,通过非共价键作用与聚合酶结合,在建立反应时可有效封闭聚合酶活性。Q5 热启动聚合酶在常规热循环条件下才能发挥作用,因此可在室温条件下建立反应体系。Q5 热启动 DNA 聚合酶无需额外的高温激活步骤,从而减少了样品降解的可能性,缩短了反应时间,使操作更为简便。无论 GC 含量高低,Q5 热启动超保真 DNA 聚合酶都能表现出高特异性扩增性能和广泛的模板适应性
Q5 反应缓冲液系统使扩增范围更广泛,无论模板 GC 含量的高低,Q5 超保真 DNA 聚合酶均以最少的优化,表现出卓越的扩增能力。对于常规或 GC 含量 ≤ 65% 的复杂模板,使用 Q5 反应缓冲液提供了可靠的、超强的扩增能力;对于高 GC 含量的模板(> 65%),添加 Q5 High GC Enhancer 确保最大的扩增性能。
Q5超保真DNA聚合酶有无可匹敌的扩增保真性(> Taq 酶的 100 倍)及其超低的错配率,携带具有持续合成能力的Sso7d DNA结合域,能够提高反应速度、保真度和可靠性。速度快产量高,扩增范围更广泛。延伸速率高达6kb/min,扩增长度<=20kb,保真度> 100X Taq,产物平末端。添加Q5 High GC Enhancer可确保高GC含量模板(> 65%)的最大扩增性能。热启动

LongAmp® 热启动 Taq DNA 聚合酶:LongAmp Hot Start Taq DNA Polymerase 500 units

价格:¥

货号:M0534S
品牌:NEB

经销:基因有限公司

扩增长度≤30kb;保真度:2x Taq;延伸速率1.2kb/min;产物末端:3'A、平末端。是 Taq 和 Deep VentRTM DNA 聚合酶的优化混合,相比单用 Taq DNA 聚合酶,它能以更高的保真度扩增出更长的 PCR 产物。
Taq和Deep Vent DNA聚合酶的优化混合,能以更高的保真度扩增出更长的PCR产物。延伸速率:1.2kb/min,扩增长度<=30kb,保真度2X Taq,产物平末端3'A末端混合。5U/50ul反应体系。热启动

LongAmp® 热启动 Taq 2X Master Mix :LongAmp Hot Start Taq 2X Master Mix 100 rxns (50 μl vol)

价格:¥

货号:M0533S
品牌:NEB

经销:基因有限公司

超长扩增长度≤30kb;保真度:2x Taq;延伸速率1.2kb/min;产物末端:3'A、平末端。是 Taq 和 Deep VentRTM DNA 聚合酶的优化混合,相比单用 Taq DNA 聚合酶,它能以更高的保真度扩增出更长的 PCR 产物。LongAmp热启动Taq DNA聚合酶利用了一种特定合成的核酸适配体,它能够与酶可逆结合,当温度低于45℃ 时抑制聚合酶活性,正常的热循环条件下释放聚合酶。即用型酶预混液。
Taq和Deep Vent DNA聚合酶的优化混合,能以更高的保真度扩增出更长的PCR产物。延伸速率:1.2kb/min,扩增长度<=30kb,保真度2X Taq,产物平末端3'A末端混合。5U/50ul反应体系。热启动预混液,contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification rega

热启动 Taq DNA 聚合酶:Hot Start Taq DNA Polymerase 1,000 units

价格:¥

货号:M0495L
品牌:NEB

经销:基因有限公司

常规热启动Taq酶,延伸速度 1 kb/min;扩增长度 ≤ 5 kb;1.25 Unit/50ul反应体系;3'A末端。与化学修饰或基于抗体结合的热启动聚合酶不同,NEB 的热启动 Taq 利用一种基于核酸适配体(aptamer)技术。这种独特的核酸适配体通过非共价键作用与聚合酶结合,从而抑制聚合酶在非许可温度下的聚合反应。这种新方法不需要激活步骤,减少样品降解的可能性、缩短整个反应时间。

热启动 Taq 2X Master Mix:Hot Start Taq 2X Master Mix 500 rxns

价格:¥

货号:M0496L
品牌:NEB

经销:基因有限公司

常规热启动Taq酶预混液,延伸速度 1 kb/min;扩增长度 ≤ 5 kb;1.25 Unit/50ul反应体系;3'A末端。与化学修饰或基于抗体结合的热启动聚合酶不同,NEB 的热启动 Taq 利用一种基于核酸适配体(aptamer)技术。这种独特的核酸适配体通过非共价键作用与聚合酶结合,从而抑制聚合酶在非许可温度下的聚合反应。这种新方法不需要激活步骤,减少样品降解的可能性、缩短整个反应时间。

OneTaq® 热启动 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start 2X Master Mix with GC Buffer 500 rxns

价格:¥

货号:M0485L
品牌:NEB

经销:基因有限公司

High GC Enhancer 随 GC 缓冲液一同提供。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体

OneTaq® 热启动 2X Master Mix(提供标准缓冲液):OneTaq Hot Start 2X Master Mix with Standard Buffer 500 rxns

价格:¥

货号:M0484L
品牌:NEB

经销:基因有限公司

OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二

OneTaq® 热启动 Quick-Load 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer 500 rxns

价格:¥

货号:M0489L
品牌:NEB

经销:基因有限公司

Quick-Load形式酶预混液,可用于直接凝胶上样。High GC Enhancer 随 GC 缓冲液一同提供。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的

OneTaq® 热启动 Quick-Load® 2X Master Mix(提供标准缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer 500 rxns

价格:¥

货号:M0488L
品牌:NEB

经销:基因有限公司

Quick-Load形式酶预混液,可用于直接凝胶上样。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低,都只需最小的优化即能获得最高的产量。OneTaq 热启动 DNA 聚合酶利用核酸适配体(a

OneTaq® 热启动 DNA 聚合酶:OneTaq Hot Start DNA Polymerase 1,000 units

价格:¥

货号:M0481L
品牌:NEB

经销:基因有限公司

延伸速率1kb/min. 扩增长度≤6kb。保真度〉2X Taq; 1.25units/50ul反应体系。 产物末端结构:3'A。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二聚体和

Q5® 热启动超保真 DNA 聚合酶:Q5 Hot Start High-Fidelity DNA Polymerase 500 units

价格:¥

货号:M0493L
品牌:NEB

经销:基因有限公司

Q5 热启动超保真DNA聚合酶延伸速度 6kb/min;扩增长度 ≤ 20kb;高保真度:>100X Taq;1 Unit/50ul反应体系;平末端。Q5 无与伦比的扩增保真性(> Taq 酶的100倍)及其超低的错配率,建立了高保真聚合酶的新标准。无论延伸速度,扩增长度,保真度和可靠性都远远超过常规Taq酶。NEB的 Q5 采用了独特的核酸适配体修饰的热启动方式,通过非共价键作用与聚合酶结合,在建立反应时可有效封闭聚合酶活性。Q5 热启动聚合酶在常规热循环条件下才能发挥作用,因此可在室温条件下建立反应体系。Q5 热启动 DNA 聚合酶无需额外的高温激活步骤,从而减少了样品降解的可能性,缩短了反应时间,使操作更为简便。无论 GC 含量高低,Q5 热启动超保真 DNA 聚合酶都能表现出高特异性扩增性能和广泛的模板适应性
Q5 反应缓冲液系统使扩增范围更广泛,无论模板 GC 含量的高低,Q5 超保真 DNA 聚合酶均以最少的优化,表现出卓越的扩增能力。对于常规或 GC 含量 ≤ 65% 的复杂模板,使用 Q5 反应缓冲液提供了可靠的、超强的扩增能力;对于高 GC 含量的模板(> 65%),添加 Q5 High GC Enhancer 确保最大的扩增性能。
Q5超保真DNA聚合酶有无可匹敌的扩增保真性(> Taq 酶的 100 倍)及其超低的错配率,携带具有持续合成能力的Sso7d DNA结合域,能够提高反应速度、保真度和可靠性。速度快产量高,扩增范围更广泛。延伸速率高达6kb/min,扩增长度<=20kb,保真度> 100X Taq,产物平末端。添加Q5 High GC Enhancer可确保高GC含量模板(> 65%)的最大扩增性能。

LongAmp® 热启动 Taq DNA 聚合酶:LongAmp Hot Start Taq DNA Polymerase 2,500 units

价格:¥

货号:M0534L
品牌:NEB

经销:基因有限公司

扩增长度≤30kb;保真度:2x Taq;延伸速率1.2kb/min;产物末端:3'A、平末端。是 Taq 和 Deep VentRTM DNA 聚合酶的优化混合,相比单用 Taq DNA 聚合酶,它能以更高的保真度扩增出更长的 PCR 产物。
Taq和Deep Vent DNA聚合酶的优化混合,能以更高的保真度扩增出更长的PCR产物。延伸速率:1.2kb/min,扩增长度<=30kb,保真度2X Taq,产物平末端3'A末端混合。5U/50ul反应体系。热启动

LongAmp® 热启动 Taq 2X Master Mix :LongAmp Hot Start Taq 2X Master Mix 500 rxns (50 μl vol)

价格:¥

货号:M0533L
品牌:NEB

经销:基因有限公司

超长扩增长度≤30kb;保真度:2x Taq;延伸速率1.2kb/min;产物末端:3'A、平末端。是 Taq 和 Deep VentRTM DNA 聚合酶的优化混合,相比单用 Taq DNA 聚合酶,它能以更高的保真度扩增出更长的 PCR 产物。即用型酶预混液。
Taq和Deep Vent DNA聚合酶的优化混合,能以更高的保真度扩增出更长的PCR产物。延伸速率:1.2kb/min,扩增长度<=30kb,保真度2X Taq,产物平末端3'A末端混合。5U/50ul反应体系。热启动预混液

OneTaq® 热启动 DNA 聚合酶:OneTaq Hot Start DNA Polymerase 5,000 units

价格:¥

货号:M0481X
品牌:NEB

经销:基因有限公司

延伸速率1kb/min. 扩增长度≤6kb。保真度〉2X Taq; 1.25units/50ul反应体系。 产物末端结构:3'A。OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentRTM DNA 聚合酶的混合物,可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´ →5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二聚体和

MyFi热启动酶预混液:MyFi Mix 2x, 100 Reactions

价格:¥

货号:BIO-25049
品牌:Bioline

经销:基因有限公司

MyFi热启动PCR预混液(2x,100反应),含新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。保真性高于Taq酶3.5倍,扩增片段长达10kb,适用于高GC/AT等困难模板扩增 高保真热启动聚合酶即用型2x预混液。常温下抗体封闭Taq酶活,加热后抗体失活释放酶活,保真度比常规Taq高3.5倍,TA克隆,可扩增10kb模板。A novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

Convenient - ready-to-use, all-in-one 2x mastermix
New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications
TA Cloning
Long PCR
Low-copy PCR assays
PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)
High-fidelity PCR assays (library amplification)

Description
MyFi Mix is a ready-to-use 2x mix formulation, containing all the components (including proprietary enhancers and stabilizers) necessary for trouble-free PCR reaction assembly, while delivering the same unique balance of PCR sensitivity and high-fidelity. The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility .

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.

MyTaq热启动DNA 聚合酶:MyTaq HS DNA Polymerase 250 units

价格:¥

货号:BIO-21111
品牌:Bioline

经销:基因有限公司

MyTaq热启动PCR酶(250U),是新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。可用于快速PCR,低浓度模板、高GC模板及困难模板扩增 热启动Taq酶,Taq酶抗体在室温下封闭Taq酶活性,加热后抗体失活释放Taq酶活性。A very high-performance, antibody-mediated hot-start DNA polymerase, designed for fast, highly-specific PCR. MyTaq HS DNA Polymerase has the added convenience of allowing room temperature reaction assembly, without non-specific amplification and primer-dimer formation.
Universal DNA polymerase - recommended for most applications
Fast - complete PCR reaction in less than 30 minutes
Antibody-based hot-start polymerase - for highest specificity
Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
All-in-one mastermix available - for enhance reproducibility
Applications
Fast PCR reactions
Colony PCR
Genotyping
Multiplexing
Low-copy PCR assays
Specific amplification of difficult templates (GC-rich)
Assays with prolonged reaction set-up
Description
MyTaq HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, resulting in significant improvements in yield, sensitivity and speed. MyTaq HS has the added convenience of room temperature reaction assembly, thus avoiding non-specific amplification and primer-dimer formation. With activation times as low as 1 to 2 minutes, a complete PCR assay can be completed in less than 30 minutes .
The enzyme is supplied with an industry-leading novel buffer system, consisting of a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations. This removes the need for optimizing individual reactions and delivers superior amplification time after time

IMMOLASE DNA聚合酶:IMMOLASE DNA Polymerase 250 Units

价格:¥

货号:BIO-21046
品牌:Bioline

经销:基因有限公司

IMMOLASE DNA聚合酶(250U),是化学修饰的热启动酶。与普通聚合酶相比,该酶可有效防止室温下聚合酶引发的非特异性扩增反应,提高目的片段的扩增产物。与Bioline 2x PolyMate Additive (supplied separately)联用可用于富含GC或者AT的模板扩增。 化学修饰的热启动聚合酶常温下无活性,95度加热10分钟后活化,严格的热启动避免非特异结合扩增。5u/µl。 A heat-activated, thermostable DNA polymerase. IMMOLASE provides high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specific binding, such as primer-dimers and mis-primed products.
Higher Performance - robust polymerase
Chemical hot-start PCR enzyme - eliminates non-specific binding
Reaction requires activation step - allows convenient reaction set-up at room temperature
Suitable for TA cloning - leaves ´A´ overhang
Applications
For multiplex reactions
Products suitable for TA cloning
Description
IMMOLASE™ is a heat-activated thermostable DNA polymerase and provides high yield and improved specificity when compared to standard polymerases. IMMOLASE can eliminate the presence of non-specifics such as primer-dimers and mis-primed products.
IMMOLASE is inactive at room temperature and requires activation by heat treatment for 10 minutes prior to PCR cycling . This provides greater flexibility in reaction set ups and allows the premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to preferred protocols for thermostable DNA polymerases.
The specificity and performance of IMMOLASE can be further improved through the use of Bioline 2x PolyMate Additive (supplied separately, see related products) which is designed for GC-rich or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.

MyFi热启动 DNA 聚合酶:MyFi DNA Polymerase 250 Units

价格:¥

货号:BIO-21117
品牌:Bioline

经销:基因有限公司

MyFi热启动PCR酶(250U),是新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。保真性高于Taq酶3.5倍,扩增片段长达10kb,适用于高GC/AT等困难模板扩增 高保真热启动聚合酶。常温下抗体封闭Taq酶活,加热后抗体失活释放酶活,保真度比常规Taq高3.5倍,TA克隆,可扩增10kb模板,2u/µl。A novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

Convenient - ready-to-use, all-in-one 2x mastermix
New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications
Routine PCR applications, TA Cloning
Long PCR
Low-copy PCR assays
PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)
High throughput PCR requiring high specificity combined with higher fidelity

Description
MyFi DNA Polymerase is a high-performance, new generation DNA polymerase complex, combining the sensitivity of our ever-popular MyTaq HS DNA Polymerase with a highly processive proof-reading DNA polymerase. This unique combination coupled with an efficient antibody-based hot-start is engineered and optimized for maximum sensitivity and robust, high-fidelity PCR amplification.

MyFi has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates .

MyFi DNA Polymerase generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.

MyTaq热启动酶预混液:MyTaq HS Mix 2x, 200 Reactions

价格:¥

货号:BIO-25045
品牌:Bioline

经销:基因有限公司

MyTaq热启动PCR预混液(2x,200反应),含新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。可用于快速PCR,低浓度模板、高GC模板及困难模板扩增,有单酶形式可供选择 MyTaq HS Mix, 2x, 200 Reactions 热启动PCR预混液 ,200次

MyTaq 热启动酶预混液(红色):MyTaq HS Red Mix 2x, 200 Reactions

价格:¥

货号:BIO-25047
品牌:Bioline

经销:基因有限公司

MyTaq热启动PCR预混液(红色)(2x,200反应),含新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。可用于快速PCR,低浓度模板、高GC模板及困难模板扩增 带指示剂的即用型热启动2xTaq酶预混液。Taq抗体常温下封闭酶活性,加热后抗体失活释放酶,提高特异性。自带指示剂便于识别,补影响后继反应,PCR后可直接上样电泳。A ready-to-use, direct gel load, red colored, 2x mix for fast, highly-specific hot-start PCR. MyTaq HS Red Mix is powered by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield.

All-in-one mastermix formulations - for enhanced reproducibility
Fast - complete PCR reaction in less than 30 minutes
Antibody-based hot-start polymerase - for highest specificity
Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
Red dye - for direct gel loading
Applications
Fast PCR reactions
Colony PCR
Genotyping
Multiplexing
Low-copy PCR assays
Specific amplification of difficult templates (GC-rich)
Assays with prolonged reaction set-up
MyTaq™ HS Red Mix is a ready-to-use, direct gel load, red colored, 2x mix for fast, highly-specific hot-start PCR. MyTaq HS Red Mix is powered by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield , making it ideal for high-throughput.
MyTaq HS Red Mix is designed to facilitate increased efficiency, throughput and reproducibility, making MyTaq HS Red Mix perfect for techniques such as for multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, even though the amplicons are different sizes.
The specially designed MyTaq Red formulation does not interfere with the PCR reaction and allows users to load samples directly onto a gel after the PCR without the need to add loading buffer, making MyTaq HS Red Mix ideal for automation. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).
MyTaq HS Red Mix only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.

IMMOLASE DNA聚合酶:IMMOLASE DNA Polymerase 500 Units

价格:¥

货号:BIO-21047
品牌:Bioline

经销:基因有限公司

IMMOLASE DNA聚合酶(500U),是化学修饰的热启动酶。与普通聚合酶相比,该酶可有效防止室温下聚合酶引发的非特异性扩增反应,提高目的片段的扩增产物。与Bioline 2x PolyMate Additive (supplied separately)联用可用于富含GC或者AT的模板扩增。 化学修饰的热启动聚合酶常温下无活性,95度加热10分钟后活化,严格的热启动避免非特异结合扩增。5u/µl。 A heat-activated, thermostable DNA polymerase. IMMOLASE provides high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specific binding, such as primer-dimers and mis-primed products.
Higher Performance - robust polymerase
Chemical hot-start PCR enzyme - eliminates non-specific binding
Reaction requires activation step - allows convenient reaction set-up at room temperature
Suitable for TA cloning - leaves ´A´ overhang
Applications
For multiplex reactions
Products suitable for TA cloning
Description
IMMOLASE™ is a heat-activated thermostable DNA polymerase and provides high yield and improved specificity when compared to standard polymerases. IMMOLASE can eliminate the presence of non-specifics such as primer-dimers and mis-primed products.
IMMOLASE is inactive at room temperature and requires activation by heat treatment for 10 minutes prior to PCR cycling . This provides greater flexibility in reaction set ups and allows the premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to preferred protocols for thermostable DNA polymerases.
The specificity and performance of IMMOLASE can be further improved through the use of Bioline 2x PolyMate Additive (supplied separately, see related products) which is designed for GC-rich or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.

MyFi热启动 DNA 聚合酶:MyFi DNA Polymerase 500 Units

价格:¥

货号:BIO-21118
品牌:Bioline

经销:基因有限公司

MyFi热启动PCR酶(500U),是新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。保真性高于Taq酶3.5倍,扩增片段长达10kb,适用于高GC/AT等困难模板扩增 高保真热启动聚合酶。常温下抗体封闭Taq酶活,加热后抗体失活释放酶活,保真度比常规Taq高3.5倍,TA克隆,可扩增10kb模板,2u/µl。A novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

Convenient - ready-to-use, all-in-one 2x mastermix
New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications
Routine PCR applications, TA Cloning
Long PCR
Low-copy PCR assays
PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)
High throughput PCR requiring high specificity combined with higher fidelity

Description
MyFi DNA Polymerase is a high-performance, new generation DNA polymerase complex, combining the sensitivity of our ever-popular MyTaq HS DNA Polymerase with a highly processive proof-reading DNA polymerase. This unique combination coupled with an efficient antibody-based hot-start is engineered and optimized for maximum sensitivity and robust, high-fidelity PCR amplification.

MyFi has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates .

MyFi DNA Polymerase generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.

MyTaq热启动DNA 聚合酶:MyTaq HS DNA Polymerase 1000 units

价格:¥

货号:BIO-21112
品牌:Bioline

经销:基因有限公司

MyTaq热启动PCR酶(1000U),是新型的抗体介导的热启动酶,有效防止室温条件下的非特异性扩增,提高目的片段产量。可用于快速PCR,低浓度模板、高GC模板及困难模板扩增 热启动Taq酶,Taq酶抗体在室温下封闭Taq酶活性,加热后抗体失活释放Taq酶活性。A very high-performance, antibody-mediated hot-start DNA polymerase, designed for fast, highly-specific PCR. MyTaq HS DNA Polymerase has the added convenience of allowing room temperature reaction assembly, without non-specific amplification and primer-dimer formation.
Universal DNA polymerase - recommended for most applications
Fast - complete PCR reaction in less than 30 minutes
Antibody-based hot-start polymerase - for highest specificity
Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
All-in-one mastermix available - for enhance reproducibility
Applications
Fast PCR reactions
Colony PCR
Genotyping
Multiplexing
Low-copy PCR assays
Specific amplification of difficult templates (GC-rich)
Assays with prolonged reaction set-up
Description
MyTaq HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, resulting in significant improvements in yield, sensitivity and speed. MyTaq HS has the added convenience of room temperature reaction assembly, thus avoiding non-specific amplification and primer-dimer formation. With activation times as low as 1 to 2 minutes, a complete PCR assay can be completed in less than 30 minutes .
The enzyme is supplied with an industry-leading novel buffer system, consisting of a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations. This removes the need for optimizing individual reactions and delivers superior amplification time after time

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