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产品名/货号/品牌 简介 包装  
EpiMark Hot Start Taq DNA Polymerase, 100 rxns (50 μl vol) 100 rxns

价格:¥

货号:M0490S
品牌:NEB

经销:基因有限公司

EpiMark 热启动 Taq DNA 聚合酶是进行重亚硫酸盐处理的 DNA 操作的绝佳选择。该酶是 Taq DNA 聚合酶和一种对温度敏感的适配体抑制剂的混合物。该抑制剂能够与聚合酶可逆结合,在 45℃ 以下抑制聚合酶活性,而在 PCR 热循环条件下又可以释放聚合酶,所以该酶可以在室温下操作却不需要高温步骤来激活聚合酶。延伸速率 1kb/min。扩增长度≤ 1 kb。1.25 Units/50ul反应体系。产物末端:3'A。

EpiMark Hot Start Taq DNA Polymerase, 500 rxns (50 μl vol) 500 rxns

价格:¥

货号:M0490L
品牌:NEB

经销:基因有限公司

EpiMark 热启动 Taq DNA 聚合酶是进行重亚硫酸盐处理的 DNA 操作的绝佳选择。该酶是 Taq DNA 聚合酶和一种对温度敏感的适配体抑制剂的混合物。该抑制剂能够与聚合酶可逆结合,在 45℃ 以下抑制聚合酶活性,而在 PCR 热循环条件下又可以释放聚合酶,所以该酶可以在室温下操作却不需要高温步骤来激活聚合酶。延伸速率 1kb/min。扩增长度≤ 1 kb。1.25 Units/50ul反应体系。产物末端:3'A。

BIO-X-ACT Short DNA Polymerase, 500 Units 500units

价格:¥

货号:BIO-21065
品牌:Bioline

经销:基因有限公司

BIO-X-ACT截短型PCR酶(500U),扩增片段长达5kb,有PCR预混液产品形式,适用于高GC含量模板、肮脏模板等问题模板扩增 适合困难模板的高性能Taq酶,如GC丰富的基因组模板,法医样本,粗制样本,能Taq所不能。a high-performance enzyme designed for difficult or problematic PCR applications. BIO-X-ACT Short is recommended for short genomic DNA fragments of up to 3kb. When using Lambda DNA as template, the best performance is achieved within the 100bp-5kb range.
High performance - for templates that fail with standard Taq DNA Polymerases
For problematic templates - can work with high GC content, dirty templates or difficult melting profiles
Good for short amplicons - amplifies genomic fragments up to 3kb
Flexible format - Available as a ready-to-use 2x reaction mix (BIO-X-ACT™ Short Mix) Applications
Suitable for TA cloning
GC-rich templates
Crude sample PCR
Forensic applications
Description
BIO-X-ACT™ Short DNA Polymerase is a high-performance enzyme designed for difficult or problematic PCR applications. BIO-X-ACT is the polymerases of choice for difficult applications that would normally fail with standard Taq polymerases .
BIO-X-ACT Short is recommended for short genomic DNA fragments up to 3kb and Lambda DNA up to 5kb.
For enhanced specificity, BIO-X-ACT Short is supplied with a vial of Hi-Spec Additive. Hi-Spec Additive is a very efficient enhancer, which helps to reduce the formation of false background bands and smearing. The specificity and performance can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.

BIO-X-ACT Short DNA Polymerase Mix, 2x, 500 Reactions 500reactions

价格:¥

货号:BIO-25026
品牌:Bioline

经销:基因有限公司

BIO-X-ACT截短型PCR预混液(2x,500反应),扩增片段长达5kb,也有单酶产品形式,适用于高GC含量模板、肮脏模板等问题模板扩增 适合困难模板的高性能即用型2x Taq酶预混液,如GC丰富的基因组模板,法医样本,粗制样本,能Taq所不能。A high-performance, complete, ready-to-use, 2x reaction mix that enables PCR assays to be performed on problematic templates through the simple addition of water, template and primers. BIO-X-ACT Short is recommended for short genomic DNA fragments of up to 3kb. When using Lambda DNA as template, the best performance is achieved within the 100bp-5kb range.

High performance - for templates that fail with standard Taq DNA Polymerases
For problematic templates - can work with high GC content, dirty templates or difficult melting profiles
Good for short amplicons - amplifies genomic fragments up to 3kb
Convenient - pre-mixed, pre-optimized 2x solution
Ready to use - reduces risk of contamination

BIO-X-ACT™ Short Mix is a complete, ready-to-use, 2x reaction mix which enables PCR assays on problematic templates, simply by adding water, template and primers. BIO-X-ACT Short is recommended for short genomic DNA fragments up to 3kb and Lambda DNA up to 5kb.
To achieve optimal reaction conditions, the BIO-X-ACT Short Mix contains BIO-X-ACT™ Short DNA Polymerase, MgCl2, ultra-pure dNTPs manufactured by Bioline, as well as additives. The mix has been optimized for a wide variety of templates and an additional 50mM MgCl2 solution is included, should fine-tuning be required.
BIO-X-ACT Short Mix reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured through a reduction in the number of pipetting steps that can introduce errors.

EPIK Amplification Kit, 200 Reactions 200reactions

价格:¥

货号:BIO-66025
品牌:Bioline

经销:基因有限公司

甲基化模板PCR试剂盒(200x50ul反应),是一款专门针对甲基化模板经重亚硫酸盐转化后的模板扩增试剂盒。该试剂盒显著改善由于重亚硫酸盐转化、含尿嘧啶的DNA模板等对PCR造成的抑制,增加产量,提高反应灵敏性和特异性 新一代热启动聚合酶试剂盒,特殊缓冲液配方适合含尿嘧啶、重亚硫酸修饰的模板、表观分析、 Pyrosequencing应用。高产New generation hot-start PCR kit specifically engineered to deliver highly reliable, ultra-sensitive amplification from bisulfite-modified template DNA. EPIK Amplification Kit features a novel buffer system, EPIKORE™ that has been specially engineered to overcome problems associated with bisulfite-modified, uracil-containing DNA templates, to offer significant improvements in reliability, yield and sensitivity compared to existing epigenetic kits on the market.

Outstanding reliability - engineered for best-in-class epigenetic analysis time after time
Unique EPIKORE buffer system - optimized for amplification of bisulfite-modified DNA
Engineered to amplify all fragments - with low, medium and high GC content without bias
Hot-start system - powered by MyTaq™ HS for high specificity
Enhanced polymerization - for longer amplicons
Higher yield - even with low DNA template

Applications
Bisulfite-modified, uracil containing DNA (up to 1.5kb)
Difficult DNA templates e.g. GC-rich
Bisulfite-restriction PCR e.g. COBRA
Bisulfite-sequencing PCR (Sanger, oxBS-seq, TAB-seq, NOME-seq)
Bisulfite NGS library preparation (Whole Genome, RRBS)
Pyrosequencing assays
TA cloning

Description
EPIK Amplification Kit has been specifically engineered for amplification of bisulphite-modified DNA and features a the dedicated buffer system (EPIKORE™), designed to overcome the problems associated with bisulfite-modified, uracil-containing DNA templates such as template degradation and uracil stalling. EPIK Amplification Kit offers significant improvements in reliability, yield and sensitivity leading to far greater PCR success rates.

EPIK delivers truly unrivalled, market-leading performance, even with longer amplicons (1.5kb) . Furthermore, EPIK offers significantly improved amplification success rates even with low template concentrations (<0.5ng) of bisulfite-modified DNA .

Powered by MyTaq™ HS and EPIKORE™ technology, the high speed and enhanced specificity of EPIK™ Amplification Kit makes it highly suited for high-throughput epigenetic assays and the very latest bisulfite sequencing applications.

EPIK Amplification Kit, 500 Reactions 500reactions

价格:¥

货号:BIO-66026
品牌:Bioline

经销:基因有限公司

甲基化模板PCR试剂盒(500x50ul反应),是一款专门针对甲基化模板经重亚硫酸盐转化后的模板扩增试剂盒。该试剂盒显著改善由于重亚硫酸盐转化、含尿嘧啶的DNA模板等对PCR造成的抑制,增加产量,提高反应灵敏性和特异性 新一代热启动聚合酶试剂盒,特殊缓冲液配方适合含尿嘧啶、重亚硫酸修饰的模板, Pyrosequencing应用。New generation hot-start PCR kit specifically engineered to deliver highly reliable, ultra-sensitive amplification from bisulfite-modified template DNA. EPIK Amplification Kit features a novel buffer system, EPIKORE™ that has been specially engineered to overcome problems associated with bisulfite-modified, uracil-containing DNA templates, to offer significant improvements in reliability, yield and sensitivity compared to existing epigenetic kits on the market.

Outstanding reliability - engineered for best-in-class epigenetic analysis time after time
Unique EPIKORE buffer system - optimized for amplification of bisulfite-modified DNA
Engineered to amplify all fragments - with low, medium and high GC content without bias
Hot-start system - powered by MyTaq™ HS for high specificity
Enhanced polymerization - for longer amplicons
Higher yield - even with low DNA template

Applications
Bisulfite-modified, uracil containing DNA (up to 1.5kb)
Difficult DNA templates e.g. GC-rich
Bisulfite-restriction PCR e.g. COBRA
Bisulfite-sequencing PCR (Sanger, oxBS-seq, TAB-seq, NOME-seq)
Bisulfite NGS library preparation (Whole Genome, RRBS)
Pyrosequencing assays
TA cloning

Description
EPIK Amplification Kit has been specifically engineered for amplification of bisulphite-modified DNA and features a the dedicated buffer system (EPIKORE™), designed to overcome the problems associated with bisulfite-modified, uracil-containing DNA templates such as template degradation and uracil stalling. EPIK Amplification Kit offers significant improvements in reliability, yield and sensitivity leading to far greater PCR success rates.

EPIK delivers truly unrivalled, market-leading performance, even with longer amplicons (1.5kb) . Furthermore, EPIK offers significantly improved amplification success rates even with low template concentrations (<0.5ng) of bisulfite-modified DNA .

Powered by MyTaq™ HS and EPIKORE™ technology, the high speed and enhanced specificity of EPIK™ Amplification Kit makes it highly suited for high-throughput epigenetic assays and the very latest bisulfite sequencing applications.

EPIK Amplification Kit, sample size 1

价格:¥

货号:BIO-66025/S
品牌:Bioline

经销:基因有限公司

新一代热启动聚合酶试剂盒,特殊缓冲液配方适合含尿嘧啶、重亚硫酸修饰的模板, Pyrosequencing应用。New generation hot-start PCR kit specifically engineered to deliver highly reliable, ultra-sensitive amplification from bisulfite-modified template DNA. EPIK Amplification Kit features a novel buffer system, EPIKORE™ that has been specially engineered to overcome problems associated with bisulfite-modified, uracil-containing DNA templates, to offer significant improvements in reliability, yield and sensitivity compared to existing epigenetic kits on the market.

Outstanding reliability - engineered for best-in-class epigenetic analysis time after time
Unique EPIKORE buffer system - optimized for amplification of bisulfite-modified DNA
Engineered to amplify all fragments - with low, medium and high GC content without bias
Hot-start system - powered by MyTaq™ HS for high specificity
Enhanced polymerization - for longer amplicons
Higher yield - even with low DNA template

Applications
Bisulfite-modified, uracil containing DNA (up to 1.5kb)
Difficult DNA templates e.g. GC-rich
Bisulfite-restriction PCR e.g. COBRA
Bisulfite-sequencing PCR (Sanger, oxBS-seq, TAB-seq, NOME-seq)
Bisulfite NGS library preparation (Whole Genome, RRBS)
Pyrosequencing assays
TA cloning

Description
EPIK Amplification Kit has been specifically engineered for amplification of bisulphite-modified DNA and features a the dedicated buffer system (EPIKORE™), designed to overcome the problems associated with bisulfite-modified, uracil-containing DNA templates such as template degradation and uracil stalling. EPIK Amplification Kit offers significant improvements in reliability, yield and sensitivity leading to far greater PCR success rates.

EPIK delivers truly unrivalled, market-leading performance, even with longer amplicons (1.5kb) . Furthermore, EPIK offers significantly improved amplification success rates even with low template concentrations (<0.5ng) of bisulfite-modified DNA .

Powered by MyTaq™ HS and EPIKORE™ technology, the high speed and enhanced specificity of EPIK™ Amplification Kit makes it highly suited for high-throughput epigenetic assays and the very latest bisulfite sequencing applications.

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