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不限PCR产物纯化
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不限 NEB MNG Bioline USB
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产品名/货号/品牌 简介 包装  
Monarch PCR & DNA Cleanup Kit, 50 preps

价格:¥

货号:T1030S
品牌:NEB

经销:基因有限公司

PCR产物纯化和胶回收试剂盒,超高性价比,优秀的实验表现,独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch PCR & DNA Cleanup Kit, 250 preps

价格:¥

货号:T1030L
品牌:NEB

经销:基因有限公司

PCR产物纯化和胶回收试剂盒,超高性价比,优秀的实验表现,独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

NucleoTraPCR (10) 10

价格:¥

货号:740587.10
品牌:MNG

经销:基因有限公司

基于硅胶微珠的PCR产物回收试剂盒。硅胶珠吸附力强,大小一致沉降均匀,可避免长片段DNA 片段在离心过程中的断裂降解,特别适合长片段DNA(<50kb)或者大量DNA回收,不受柱载量限制。高效回收120bp以上的DNA片段。适用于从PCR反应中回收纯化DNA;或从反应混合物中浓缩与除盐操作。典型的下游应用包括:克隆、测序、PCR 与限制性酶切分析
硅胶微珠悬浮液
样本 < 400ul PCR反应混合物
回收片段大小:100bp-50kbp
典型回收效率:70– 80%
A260/A280: 1.7 – 1.9
洗脱体积:20 - 50 μL
制备时间:45 min /6 个制备
结合率 10μL悬浮硅胶珠可吸附6μg DNA

The silica-matrix based kit for PCR clean-up without columns
• Batch binding procedure
• Quantitative removal of primer and primer-dimer molecules
• High recovery for DNA fragments ≥ 120 bp
Technology Silica-maxtrix technology
Format Silica bead suspension
Sample material < 400 µL PCR reaction mixture
Fragment size 100 bp–approx. 50 kbp
Typical recovery 70–80%
A260/A280 1.7–1.9
Elution volume 20–50 µL
Preparation time 45 min/6 preps
Binding capacity 6 µg/10 µL suspension

NucleoTraP®CR suspension, buffers
10 preps for PCR clean-up - NucleoTraPCR Suspension, buffers pack of 10

NucleoTraPCR (100) 100

价格:¥

货号:740587
品牌:MNG

经销:基因有限公司

基于硅胶微珠的PCR产物回收试剂盒。硅胶珠吸附力强,大小一致沉降均匀,可避免长片段DNA 片段在离心过程中的断裂降解,特别适合长片段DNA(<50kb)或者大量DNA回收,不受柱载量限制。高效回收120bp以上的DNA片段。适用于从PCR反应中回收纯化DNA;或从反应混合物中浓缩与除盐操作。典型的下游应用包括:克隆、测序、PCR 与限制性酶切分析
硅胶微珠悬浮液
样本 < 400ul PCR反应混合物
回收片段大小:100bp-50kbp
典型回收效率:70– 80%
A260/A280: 1.7 – 1.9
洗脱体积:20 - 50 μL
制备时间:45 min /6 个制备
结合率 10μL悬浮硅胶珠可吸附6μg DNA

The silica-matrix based kit for PCR clean-up without columns
• Batch binding procedure
• Quantitative removal of primer and primer-dimer molecules
• High recovery for DNA fragments ≥ 120 bp
Technology Silica-maxtrix technology
Format Silica bead suspension
Sample material < 400 µL PCR reaction mixture
Fragment size 100 bp–approx. 50 kbp
Typical recovery 70–80%
A260/A280 1.7–1.9
Elution volume 20–50 µL
Preparation time 45 min/6 preps
Binding capacity 6 µg/10 µL suspension

NucleoTraP®CR suspension, buffers
100 preps for PCR clean-up - NucleoTraPCR Suspension, buffers pack of 100

NucleoMag 96 PCR (1 x 96) 1 x 96

价格:¥

货号:744100.1
品牌:MNG

经销:基因有限公司

Magnetic-bead based PCR clean-up
• Closed system: reduced risk of cross-contamination
• Small elution volumes ≥ 25 μL
• Recovery does not depend on elution volume
• Easily adapted to automated use

Technology: Magnetic-bead technology
Format: Highly reactive superparamagnetic beads
Processing: Manual or automated
Sample material: < 50 µL PCR reaction mixture
Fragment size: 150 bp–10 kbp
Typical recovery: 80–95%
A260/A280: 1.70–1.90
Elution volume ≥ 25 µL
Preparation time: 30–45 min/96 preps
Binding capacity: Approx. 0.3 µg/µL beads
Applications
• Manual or automated PCR clean-up
• Typical downstream application: sequencing
NucleoMag® P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers
96 preps for the purification of PCR products - NucleoMag P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers

NucleoSpin 8 PCR Clean-up (12 x 8) 12 x 8

价格:¥

货号:740668
品牌:MNG

经销:基因有限公司

8联管快速平行纯化PCR产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 30 min/6 strips
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, buffers
12 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, Elution Plate U-bottom, buffers

NucleoSpin 96 PCR Clean-up (1 x 96) 1 x 96

价格:¥

货号:740658.1
品牌:MNG

经销:基因有限公司

高通量96孔板形式平行纯化回收PCR 产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN 洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 45 min/plate
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
1 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers

NucleoMag 96 PCR (4 x 96) 4 x 96

价格:¥

货号:744100.4
品牌:MNG

经销:基因有限公司

Magnetic-bead based PCR clean-up
• Closed system: reduced risk of cross-contamination
• Small elution volumes ≥ 25 μL
• Recovery does not depend on elution volume
• Easily adapted to automated use

Technology: Magnetic-bead technology
Format: Highly reactive superparamagnetic beads
Processing: Manual or automated
Sample material: < 50 µL PCR reaction mixture
Fragment size: 150 bp–10 kbp
Typical recovery: 80–95%
A260/A280: 1.70–1.90
Elution volume ≥ 25 µL
Preparation time: 30–45 min/96 preps
Binding capacity: Approx. 0.3 µg/µL beads
Applications
• Manual or automated PCR clean-up
• Typical downstream application: sequencing
NucleoMag® P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers
4 x 96 preps for the purification of PCR products - NucleoMag P-Beads, Elution Plates U-bottom, Self-adhering PE Foils, buffers

NucleoSpin 96 PCR Clean-up (2 x 96) 2 x 96

价格:¥

货号:740658.2
品牌:MNG

经销:基因有限公司

高通量96孔板形式平行纯化回收PCR 产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN 洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 45 min/plate
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
2 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, MN Wash Plates , Elution Plates U-bottom, Self-adhering Foils, buffers

NucleoFast 96 PCR Clean-up Kit (4 x 96) 4 x 96

价格:¥

货号:743500.4
品牌:MNG

经销:基因有限公司

Cost-efficient 96-well ultrafiltration kit for PCR clean-up
• Ready-to-use DNA for sequencing and microarray spotting
• Sturdy membrane allows easy recovery of DNA
• No well-to-well cross-contamination
• Fast procedure
• Available as complete kits and as plates only
• Detergent-free membrane
• Manual or automated use

Technology: Ultrafiltration technology
Format: 96-well plates
Processing: Manual or automated, vacuum or centrifugation
Sample material: 20–300 µL
Fragment size: ≥ 150 bp
Typical recovery: 40–95%
A260/A280: 1.70–1.80
Recovery volume 25–100 µL
Preparation time 20 min/plate (for typical PCR reactions of 25 µL)
Applications: Purification of PCR products ≥ 150 bp
NucleoFast® 96 PCR Plates, Elution Plates U-bottom, Self-adhering Foil, buffers
4 x 96 preps for the purification of PCR products – NucleoFast 96 PCR Plates, Elution Plates U-bottom, Self-adhering Foils, buffers

NucleoSpin 8 PCR Clean-up Core Kit (48 x 8) 48 x 8

价格:¥

货号:740463.4
品牌:MNG

经销:基因有限公司

8联管快速平行纯化PCR产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 30 min/6 strips
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Strips, buffers
48 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, buffers

NucleoSpin 96 PCR Clean-up Core Kit (4 x 96) 4 x 96

价格:¥

货号:740464.4
品牌:MNG

经销:基因有限公司

高通量96孔板形式平行纯化回收PCR 产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN 洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 45 min/plate
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Plates, buffers
4 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, buffers

NucleoSpin 96 PCR Clean-up (4 x 96) 4 x 96

价格:¥

货号:740658.4
品牌:MNG

经销:基因有限公司

高通量96孔板形式平行纯化回收PCR 产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN 洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 45 min/plate
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
4 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, Elution Plates U-bottom, Self-adhering Foils, MN Wash Plates, buffers

NucleoSpin 8 PCR Clean-up (60 x 8) 60 x 8

价格:¥

货号:740668.5
品牌:MNG

经销:基因有限公司

8联管快速平行纯化PCR产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 30 min/6 strips
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, buffers
60 x 8 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Strips, MN Wash Plates, Racks of Tube Strips, Elution Plates U-bottom, buffers

NucleoMag 96 PCR (24 x 96) 24 x 96

价格:¥

货号:744100.24
品牌:MNG

经销:基因有限公司

Magnetic-bead based PCR clean-up
• Closed system: reduced risk of cross-contamination
• Small elution volumes ≥ 25 μL
• Recovery does not depend on elution volume
• Easily adapted to automated use

Technology: Magnetic-bead technology
Format: Highly reactive superparamagnetic beads
Processing: Manual or automated
Sample material: < 50 µL PCR reaction mixture
Fragment size: 150 bp–10 kbp
Typical recovery: 80–95%
A260/A280: 1.70–1.90
Elution volume ≥ 25 µL
Preparation time: 30–45 min/96 preps
Binding capacity: Approx. 0.3 µg/µL beads
Applications
• Manual or automated PCR clean-up
• Typical downstream application: sequencing
NucleoMag® P-Beads, Elution Plate U-bottom, Self-adhering PE Foil, buffers
24 x 96 preps for the purification of PCR products - NucleoMag P-Beads, Elution Plates U-bottom, Self-adhering PE Foils, buffers no dangerous goods according transport regulations

SureClean Plus, 1 x 5ml 5ml

价格:¥

货号:BIO-37047
品牌:Bioline

经销:基因有限公司

PCR产物纯化试剂盒,无柱方法,75bp以上片段回收效率98%以上,高效去除引物、dNTPs和酶,适用于DNA或dsRNA的纯化或浓缩;试剂盒中同时含粉红色共沉淀剂,方便对核酸pellets进行可视化监测 便宜好用的DNA,dsRNA片段纯化试剂,不需昂贵的硅胶膜纯化柱,也无需玻璃奶或硅胶珠,可用于纯化大于75bp的PCR产物,酶切产物,dsRNA,回收率可达98%,产物纯度高,可用于下游应用。可选用粉色沉淀助剂指示沉淀块,不影响下游应用如PCR,克隆和酶反应等。操作简单,只需在样品中加入SureClean Plus,静置10分钟后高速离心沉淀,用70%乙醇洗涤沉淀,最后再次离心沉淀,干燥后即可将沉淀重新溶解于所需的溶液里。a novel, inexpensive solution that provides a column-free method for nucleic acid purification. SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests with excellent recovery rates. SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.

Rapid - column-free PCR clean-up
Efficient - post-PCR recovery up to 98%
Optional pink dye - for improved visibility and minimal pellet loss
Pure - isolated products are suitable for downstream applications

Applications
Removes primers, non-specifics, dNTPs and enzymes
DNA or dsRNA purification or concentration
Buffer exchange

Description
SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.

Using a simple and rapid procedure, SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.

A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns (fig. 1). SureClean Plus purifies nucleic acids without the use of chaotropic salts that often contribute to denaturation of the DNA duplex.

SureClean Plus enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit your downstream experiments.

Optional Pink Co-Precipitant
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids.The co-precipitant does not interfere with downtown applications and so SureClean Plus is suitable for use in all cloning, standard PCR, real-time PCR and other enzymatic reactions.

Notes Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean solution without pink co-precipitant is recommended.

SureClean Plus, 2 x 12.5ml 25ml

价格:¥

货号:BIO-37048
品牌:Bioline

经销:基因有限公司

便宜好用的DNA,dsRNA片段纯化试剂,不需昂贵的硅胶膜纯化柱,也可免去玻璃奶或硅胶珠干燥的困扰,可用于纯化大于75bp的PCR产物,酶切产物,dsRNA,回收率可达98%,产物纯度高,可用于下游应用。可选用粉色沉淀助剂指示沉淀块,不影响下游应用如PCR,克隆和酶反应等。操作简单,只需在样品中加入SureClean Plus,静置10分钟后高速离心沉淀,用70%乙醇洗涤沉淀,最后再次离心沉淀,干燥后即可将沉淀重新溶解于所需的溶液里。a novel, inexpensive solution that provides a column-free method for nucleic acid purification. SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests with excellent recovery rates. SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.

Rapid - column-free PCR clean-up
Efficient - post-PCR recovery up to 98%
Optional pink dye - for improved visibility and minimal pellet loss
Pure - isolated products are suitable for downstream applications

Applications
Removes primers, non-specifics, dNTPs and enzymes
DNA or dsRNA purification or concentration
Buffer exchange

Description
SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.

Using a simple and rapid procedure, SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.

A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns (fig. 1). SureClean Plus purifies nucleic acids without the use of chaotropic salts that often contribute to denaturation of the DNA duplex.

SureClean Plus enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit your downstream experiments.

Optional Pink Co-Precipitant
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids.The co-precipitant does not interfere with downtown applications and so SureClean Plus is suitable for use in all cloning, standard PCR, real-time PCR and other enzymatic reactions.

Notes Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean solution without pink co-precipitant is recommended.

ExoSAP PCR产物纯化试剂盒

价格:¥

货号:7825040UL
品牌:USB

经销:基因有限公司

ExoSAP-IT For PCR Product Clean-Up 单管酶法去除PCR体系中多余的引物和dNTP,40ul

PCR产物预测序纯化试剂盒

价格:¥

货号:709951KT
品牌:USB

经销:基因有限公司

PCR Product Pre-Sequencing Kit PCR产物纯化,去除引物、dNTP等以用于一代测序。,1KT

ExoSAP PCR产物纯化试剂盒

价格:¥

货号:78200200UL
品牌:USB

经销:基因有限公司

ExoSAP-IT For PCR Product Clean-Up 单管酶法去除PCR体系中多余的引物和dNTP,200ul

PCR产物预测序纯化试剂盒

价格:¥

货号:709961KT
品牌:USB

经销:基因有限公司

PCR Product Pre-Sequencing Kit PCR产物纯化,去除引物、dNTP等以用于一代测序。,1KT

ExoSAP PCR产物纯化试剂盒

价格:¥

货号:782011ML
品牌:USB

经销:基因有限公司

ExoSAP-IT For PCR Product Clean-Up 单管酶法去除PCR体系中多余的引物和dNTP,1ml

ExoSAP PCR产物纯化试剂盒

价格:¥

货号:782024X1ML
品牌:USB

经销:基因有限公司

ExoSAP-IT For PCR Product Clean-Up 单管酶法去除PCR体系中多余的引物和dNTP,4X1ml

NucleoSpin 96 PCR Clean-up (24 x 96) 24 x 96

价格:¥

货号:740658.24
品牌:MNG

经销:基因有限公司

高通量96孔板形式平行纯化回收PCR 产物,节约时间。完全去除引物与引物二聚体,可选择真空抽滤或离心方式。创新的MN 洗脱板将孔与孔的交叉污染的可能性降低到最低;适合手工操作与自动化平台
单孔样品体积:<100ul
回收片段大小:50 bp–10 kbp
洗脱体积:75–150 µL
单孔载量:15 µg

Clean-up of PCR products – flexible 8-well strip format for varying throughput and proven 96-well plate format for high troughput
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers – optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin® 8/96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.

Sample material < 100 µL PCR reaction mixture
Fragment size 50 bp–10 kbp
Typical recovery 75–95 %
A260/A280 1.7–1.8
Elution volume 75–150 µL
Preparation time 45 min/plate
Binding capacity 15 µg
NucleoSpin® PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
24 x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, MN Wash Plates, Elution Plates U-bottom, Self-adhering Foils, buffers

ExoSAP PCR产物纯化试剂盒

价格:¥

货号:7820510ML
品牌:USB

经销:基因有限公司

ExoSAP-IT For PCR Product Clean-Up 单管酶法去除PCR体系中多余的引物和dNTP,10ml

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