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产品名/货号/品牌 简介 包装  
Luna® Universal Probe One-Step RT-qPCR Kit, 200 rxns 1Kit

价格:¥

货号:E3006S
品牌:NEB

经销:基因有限公司

NEB Luna Universal One-Step RT-qPCR Kit 是一步法 RT-qPCR 试剂盒,经过优化的试剂盒可以对目标 RNA 序列进行实时荧光定量检测,该试剂盒适 用于具有 SYBR®/FAM 通道的大部分 qPCR 设备。One-Step RT-qPCR Kit 为 RNA 检测和定量提供了方便、强大的方法。首先,RNA 在逆转录酶的作 用下被反转录为 cDNA,然后 cDNA 在 DNA 聚合酶的扩增作用下通过 qPCR 完成定量检测,并且都是在一管内完成。

Luna® Universal One-Step RT-qPCR Kit, 200 rxns 1Kit

价格:¥

货号:E3005S
品牌:NEB

经销:基因有限公司

NEB Luna Universal One-Step RT-qPCR Kit 是一步法 RT-qPCR 试剂盒,经过优化的试剂盒可以对目标 RNA 序列进行实时荧光定量检测,该试剂盒适 用于具有 SYBR®/FAM 通道的大部分 qPCR 设备。One-Step RT-qPCR Kit 为 RNA 检测和定量提供了方便、强大的方法。首先,RNA 在逆转录酶的作 用下被反转录为 cDNA,然后 cDNA 在 DNA 聚合酶的扩增作用下通过 qPCR 完成定量检测,并且都是在一管内完成。

Luna® Universal Probe One-Step RT-qPCR Kit, 500 rxns 1Kit

价格:¥

货号:E3006L
品牌:NEB

经销:基因有限公司

NEB Luna Universal One-Step RT-qPCR Kit 是一步法 RT-qPCR 试剂盒,经过优化的试剂盒可以对目标 RNA 序列进行实时荧光定量检测,该试剂盒适 用于具有 SYBR®/FAM 通道的大部分 qPCR 设备。One-Step RT-qPCR Kit 为 RNA 检测和定量提供了方便、强大的方法。首先,RNA 在逆转录酶的作 用下被反转录为 cDNA,然后 cDNA 在 DNA 聚合酶的扩增作用下通过 qPCR 完成定量检测,并且都是在一管内完成。

Luna® Universal One-Step RT-qPCR Kit, 500 rxns 1Kit

价格:¥

货号:E3005L
品牌:NEB

经销:基因有限公司

NEB Luna Universal One-Step RT-qPCR Kit 是一步法 RT-qPCR 试剂盒,经过优化的试剂盒可以对目标 RNA 序列进行实时荧光定量检测,该试剂盒适 用于具有 SYBR®/FAM 通道的大部分 qPCR 设备。One-Step RT-qPCR Kit 为 RNA 检测和定量提供了方便、强大的方法。首先,RNA 在逆转录酶的作 用下被反转录为 cDNA,然后 cDNA 在 DNA 聚合酶的扩增作用下通过 qPCR 完成定量检测,并且都是在一管内完成。

SensiFAST Probe Hi-ROX One-Step Kit, 100 rxns 100 rxns

价格:¥

货号:BIO-77001
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(Hi-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 通用的探针法单管实时RT-PCR试剂盒,包括Taqman,分子信标,Scorpions探针。从RNA样本中快速精确灵敏地定量基因表达。适合标准或者快速循环模式。热启动技术减少非特异扩增。预混ROX。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology. It uses the latest advances in buffer chemistry, together with a reverse transcriptase and hot-start DNA polymerase system to produce fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.

Accurate quantification - perfect for calculating relative gene expression from RNA samples
Ultra-sensitive - unique buffer chemistry gives superior sensitivity of low copy targets
Rapid - optimized proprietary enyzme and buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Description
SensiFAST Probe Hi-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes.

SensiFAST Probe Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also give SensiFAST Probe Hi-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Hi-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Hi-ROX One-Step Kit also contains pre-mixed ROX for optional use.

SensiFAST Probe Lo-ROX One-Step Kit, 100 rxns 100 rxns

价格:¥

货号:BIO-78001
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(Lo-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 通用的探针法单管实时RT-PCR试剂盒,如Taqman,分子信标,Scorpions探针。从RNA样本中快速精确灵敏地定量基因表达。适合标准或者快速循环模式。热启动技术减少非特异扩增。预混ROX。适用于ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™。20ul体系,100次。For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology, using the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe Lo-ROX One-Step produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.

Accurate Quantification - perfect for calculating relative gene expression from RNA samples
Ultra-Sensitive - unique buffer chemistry gives superior sensitivity of low copy targets
Rapid - optimized proprietary enzyme and buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (See product selection table), as well as other instruments that do not require the use of a passive reference.

Description
SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe Lo-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also gives SensiFAST Probe Lo-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Lo-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Lo-ROX One-Step Kit also contains premixed ROX for optional use.

SensiFAST Probe No-ROX One-Step Kit, 100 rxns 100 rxns

价格:¥

货号:BIO-76001
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(No-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 探针法单管实时RT-PCR试剂盒,快速精确灵敏,适用各种探针法。抗体介导的热启动聚合酶可减少非特异扩增,单管内完成反转录和PCR定量。不带ROX。适用Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, iCycler®, MyiQ™, iQ™5, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000,Rotor-Gene™ 6000, Rotor-Gene™Q, Eppendorf Mastercycler® ep realplex and Techne Quantica®, Illumina Eco™, Analytik Jena qTOWER 2, Techne Quantca®, PrimeQ. 20ul体系,100次反应。
For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology The use of the latest advances in buffer chemistry, together with a reverse transcriptase and hot-start DNA polymerase system, ensure that the kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.

Accurate Quantification - perfect for calculating relative gene expression from RNA samples
Ultra-Sensitive - unique buffer chemistry gives superior sensitivity of low copy targets
Rapid - optimized proprietary enyzme and buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, iCycler®, MyiQ™, iQ™5, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000,Rotor-Gene™ 6000, Rotor-Gene™Q, Eppendorf Mastercycler® ep realplex and Techne Quantica®, Illumina Eco™, Analytik Jena qTOWER 2, Techne Quantca®, PrimeQ. (See product selection table).

Description
SensiFAST Probe No-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments.

The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes. The SensiFAST Probe No-ROX One-Step Kit is formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity (Fig. 1).

The advanced buffer chemistry and enhancers also gives SensiFAST Probe No-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe No-ROX One-Step Kit consists of 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe No-ROX One-Step Kit is used with real-time PCR instruments that do not require a passive reference.

SensiFAST SYBR Hi-ROX One-Step Kit, 100 rxns 100rxns

价格:¥

货号:BIO-73001
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(Hi-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,预混ROX内参,可重复性好,灵敏度高。20ul体系,100次。适用于Thermo ABI 7000, 7300, 7700, 7900, 7900HT, 7900 HT FAST, StepOne™, StepOne™ Plus等多种机型。也可用于e Qiagen (Corbett) Rotor-Gene™6000, the Bio-Rad CFX96 or the Roche LightCycler®480等不需ROX的机型. For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® Hi-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR. For ease-of-use and added convenience, SensiFAST SYBR® Hi-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-usepremix, only primers and template need to be added.

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
Sensitive - achieve excellent results from difficult and low copy targets
Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT, 7900 HT FAST, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Description
SensiFAST SYBR® Hi-ROX One-Step Kit have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments.

SensiFAST SYBR® Hi-ROX One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.

The inclusion of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® Hi-ROX One-Step Kit produce fast, highly-specific and reproducible one-step rea-time RT-PCR (Fig.1). SensiFAST SYBR® Hi-ROX One-Step Kit consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® Hi-ROX One-Step Kit contains premixed ROX for optional use as a passive reference.

SensiFAST SYBR Lo-ROX One-Step Kit, 100 rxns 100rxns

价格:¥

货号:BIO-74001
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(Lo-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,预混ROX内参,可重复性好,灵敏度高。适用机型ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™等,也适用于无需ROX的仪器如 Qiagen (Corbett) Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480. 20ul反应体系,100次。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® Lo-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR. For ease-of-use and added convenience, SensiFAST SYBR® Lo-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-use premix, only primers and template need to be added.

Rapid - hot-start capability saves time and reduces primer-dimer formation
Unique buffer chemistry - for highest specificity
Broad dynamic range - for high sensitivity
Simple and reproducible - just add primers and template

Instrument Compatibility
ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™ (See product selection table), as well as other instruments that do not require the use of a passive reference, Qiagen (Corbett) Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480..

Description
SensiFAST SYBR® Lo-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation.

This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® Lo-ROX Kit produces highly-specific and reproducible (fig. 1) real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® Lo-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.

For ease-of-use, SensiFAST SYBR® Lo-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers.

SensiFAST SYBR® Lo-ROX contains premixed ROX for optional use as a passive reference.

SensiFAST SYBR No-ROX One-Step Kit, 100 rxns 100rxns

价格:¥

货号:BIO-72001
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(No-ROX,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,不带ROX,可重复性好,灵敏度高。适用于Bio-Rad Opticon™, Opticon2™, MiniOpticon,Chromo4™, CFX96, CFX384;Cepheid SmartCycler™;Qiagen Rotor-Gene™ 3000 & 6000 Eppendorf Mastercycler® ep realplex;Roche LightCycler® 480;Techne Quantica® ;PCRmax Eco™;Takara Thermal Cycler Dice®(TP800) 。20ul反应体系,100次。 For fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR® Lo-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR. For ease-of-use and added convenience, SensiFAST SYBR® No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-use premix, only primers and template need to be added.

Rapid - hot-start capability saves time and reduces primer-dimer formation
Unique buffer chemistry - for highest specificity
Broad dynamic range - for high sensitivity
Simple and reproducible - just add primers and template

Instrument Compatibility
Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER and Techne Quantica®, PrimeQ.

Description
SensiFAST SYBR® No-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments.
The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn allowing greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and reproducible (fig. 1) real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.
For added convenience, SensiFAST SYBR® No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers.
SensiFAST SYBR® No-ROX is used with real-time PCR instruments that do not require a passive reference.

SensiFAST Probe Hi-ROX One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-77005
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(Hi-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 通用的探针法单管实时RT-PCR试剂盒,包括Taqman,分子信标,Scorpions探针。从RNA样本中快速精确灵敏地定量基因表达。适合标准或者快速循环模式。热启动技术减少非特异扩增。预混ROX。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology. It uses the latest advances in buffer chemistry, together with a reverse transcriptase and hot-start DNA polymerase system to produce fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.

Accurate quantification - perfect for calculating relative gene expression from RNA samples
Ultra-sensitive - unique buffer chemistry gives superior sensitivity of low copy targets
Rapid - optimized proprietary enyzme and buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Description
SensiFAST Probe Hi-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes.

SensiFAST Probe Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also give SensiFAST Probe Hi-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Hi-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Hi-ROX One-Step Kit also contains pre-mixed ROX for optional use.

SensiFAST Probe Lo-ROX One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-78005
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(Lo-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 通用的探针法单管实时RT-PCR试剂盒,如Taqman,分子信标,Scorpions探针。从RNA样本中快速精确灵敏地定量基因表达。适合标准或者快速循环模式。热启动技术减少非特异扩增。预混ROX。适用于ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™。20ul体系,500次反应。For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology, using the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe Lo-ROX One-Step produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.

Accurate Quantification - perfect for calculating relative gene expression from RNA samples
Ultra-Sensitive - unique buffer chemistry gives superior sensitivity of low copy targets
Rapid - optimized proprietary enzyme and buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (See product selection table), as well as other instruments that do not require the use of a passive reference.

Description
SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe Lo-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also gives SensiFAST Probe Lo-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Lo-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Lo-ROX One-Step Kit also contains premixed ROX for optional use.

超快探针一步法定量PCR/qPCRSensiFAST Probe No-ROX One-Step Kit, 500 rxns 1

价格:¥

货号:BIO-76005
品牌:Bioline

经销:基因有限公司

SensiFAST探针一步法定量PCR试剂盒(No-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SensiFAST Probe No-ROX One-Step Kit, 500 rxns 超快探针一步法定量PCR,500次

SensiFAST SYBR Hi-ROX One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-73005
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(Hi-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,预混ROX内参,可重复性好,灵敏度高。20ul体系,500次。适用于Thermo ABI 7000, 7300, 7700, 7900, 7900HT, 7900 HT FAST, StepOne™, StepOne™ Plus等多种机型。也可用于e Qiagen (Corbett) Rotor-Gene™6000, the Bio-Rad CFX96 or the Roche LightCycler®480等不需ROX的机型. For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® Hi-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR. For ease-of-use and added convenience, SensiFAST SYBR® Hi-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-usepremix, only primers and template need to be added.

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
Sensitive - achieve excellent results from difficult and low copy targets
Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT, 7900 HT FAST, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Description
SensiFAST SYBR® Hi-ROX One-Step Kit have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments.

SensiFAST SYBR® Hi-ROX One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.

The inclusion of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® Hi-ROX One-Step Kit produce fast, highly-specific and reproducible one-step rea-time RT-PCR (Fig.1). SensiFAST SYBR® Hi-ROX One-Step Kit consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® Hi-ROX One-Step Kit contains premixed ROX for optional use as a passive reference.

SensiFAST SYBR Lo-ROX One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-74005
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(Lo-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,预混ROX内参,可重复性好,灵敏度高。适用机型ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™等,也适用于无需ROX的仪器如 Qiagen (Corbett) Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.20ul反应体系,500次。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR® Lo-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR. For ease-of-use and added convenience, SensiFAST SYBR® Lo-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-use premix, only primers and template need to be added.

Rapid - hot-start capability saves time and reduces primer-dimer formation
Unique buffer chemistry - for highest specificity
Broad dynamic range - for high sensitivity
Simple and reproducible - just add primers and template

Instrument Compatibility
ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™ (See product selection table), as well as other instruments that do not require the use of a passive reference, Qiagen (Corbett) Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480..

Description
SensiFAST SYBR® Lo-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation.

This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® Lo-ROX Kit produces highly-specific and reproducible (fig. 1) real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® Lo-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.

For ease-of-use, SensiFAST SYBR® Lo-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers.

SensiFAST SYBR® Lo-ROX contains premixed ROX for optional use as a passive reference.

SensiFAST SYBR No-ROX One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-72005
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(No-ROX,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶能实现单管内分步完成反转录和定量PCR,有效减少非特异结合竞争,提高定量精确性,不带ROX,可重复性好,灵敏度高。适用于Bio-Rad Opticon™, Opticon2™, MiniOpticon,Chromo4™, CFX96, CFX384;Cepheid SmartCycler™;Qiagen Rotor-Gene™ 3000 & 6000 Eppendorf Mastercycler® ep realplex;Roche LightCycler® 480;Techne Quantica® ;PCRmax Eco™;Takara Thermal Cycler Dice®(TP800) 。20ul反应体系,500次。 For fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR® Lo-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR. For ease-of-use and added convenience, SensiFAST SYBR® No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-use premix, only primers and template need to be added.

Rapid - hot-start capability saves time and reduces primer-dimer formation
Unique buffer chemistry - for highest specificity
Broad dynamic range - for high sensitivity
Simple and reproducible - just add primers and template

Instrument Compatibility
Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER and Techne Quantica®, PrimeQ.

Description
SensiFAST SYBR® No-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments.
The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn allowing greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and reproducible (fig. 1) real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.
For added convenience, SensiFAST SYBR® No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers.
SensiFAST SYBR® No-ROX is used with real-time PCR instruments that do not require a passive reference.

HiScript® II One Step qRT-PCR Probe Kit 100 rxn (50 μl/rxn)

价格:¥

货号:Q222-01
品牌:Vazyme

经销:南京诺唯赞生物

低拷贝数模板或弱阳性样本的检出率仍然是临床诊断的挑战之一。HiScript® II One Step qRT-PCR Probe Kit含有最佳比例的HiScript® II Reverse Transcriptase和ChampagneTM Taq DNA Polymerase,以及充分优化的缓冲体系,无论是总RNA,还是体外转录RNA,或者是病毒RNA,HiScript® II One Step qRT-PCR Probe Kit都具有极高的灵敏度,并且不需要过多优化,就可以实现多重扩增。

HiScript® II One Step qRT-PCR SYBR® Green Kit 100 rxn (50 μl/rxn)

价格:¥

货号:Q221-01
品牌:Vazyme

经销:南京诺唯赞生物

HiScript® II One Step qRT-PCR SYBR® Green Kit采用SYBR®  Green I 嵌合荧光法,专为以RNA为模板(如RNA病毒)的定量PCR检测而设计。使用基因特异引物 (GSP),逆转录和PCR反应在一管内完成,不需要额外的开管/移液操作,大大提高了检测通量,并降低了污染的风险。整合HiScript® II Reverse Transcriptase以及Champagne TaqTM DNA Polymerase的优越性能,配合经过优化的缓冲体系,One Ste

HiScript® II U+ One Step qRT-PCR Probe Kit 100 rxn (50 μl/rxn)

价格:¥

货号:Q223-01
品牌:Vazyme

经销:南京诺唯赞生物

dUTP/UDG防污染系统是一种非常有效的控制PCR扩增产物污染的手段。在以DNA为模板的PCR检测试剂中,已逐渐成为了一种强制标准。不过,常用的E. coli UDG在通常的逆转录温度 (42-55℃) 下仍具有较高的活性,会降解cDNA,导致One Step RT-PCR/qPCR的灵敏度降低。HiScript® II U+ One Step qRT-PCR Probe Kit含有比例优化的dUTP/dNTP mix,并加入了来源于嗜冷海洋细菌的Heat-labile UDG。Heat-labile

SensiFAST SYBR & Fluorescein One-Step Kit, 100 rxns 100rxns

价格:¥

货号:BIO-75001
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(SYBR & Fluorescein,100x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶可减少引物二聚体和非特异竞争,快速,专一,灵敏,可重复性好。适用于Bio-Rad iCycler®, iQ™5, MyiQ™。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST SYBR® & Fluorescein One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.The SensiFAST SYBR® & Fluorescein One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
Sensitive - achieve excellent results from difficult and low copy targets
Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
Bio-Rad iCycler®, iQ™5, MyiQ™, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.

Description
SensiFAST SYBR® & Fluorescein One-Step Kit have been developed for fast real-time RT-PCR and has been validated on all commonly used real-time instruments. SensiFAST SYBR® & Fluorescein One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.

The addition of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® & Fluorescein One-Step Kit produce fast, highly-specific and reproducible one-step real-time RT-PCR (Fig. 1). SensiFAST SYBR® & Fluorescein One-Step Kit consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® & Fluorescein One-Step Kit contains premixed fluorescein for optional use as a passive reference.

SensiFAST SYBR & Fluorescein One-Step Kit, 500 rxns 500rxns

价格:¥

货号:BIO-75005
品牌:Bioline

经销:基因有限公司

SensiFAST染料一步法定量PCR试剂盒(SYBR & Fluorescein,500x20ul反应),可进行从RNA模板到定量PCR结果的一管式一步法反应,单管完成逆转录和定量两个过程,减少污染风险,快速、灵敏、适合多重定量 SYBR染料法单管实时RT-PCR试剂盒,抗体介导的热启动聚合酶可减少引物二聚体和非特异竞争,快速,专一,灵敏,可重复性好。适用于Bio-Rad iCycler®, iQ™5, MyiQ™。 For highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST SYBR® & Fluorescein One-Step Kit produces fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.The SensiFAST SYBR® & Fluorescein One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
Sensitive - achieve excellent results from difficult and low copy targets
Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling
Flexible - compatible with all standard and fast cycling instruments

Instrument Compatibility
Bio-Rad iCycler®, iQ™5, MyiQ™, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.

Description
SensiFAST SYBR® & Fluorescein One-Step Kit have been developed for fast real-time RT-PCR and has been validated on all commonly used real-time instruments. SensiFAST SYBR® & Fluorescein One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.

The addition of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® & Fluorescein One-Step Kit produce fast, highly-specific and reproducible one-step real-time RT-PCR (Fig. 1). SensiFAST SYBR® & Fluorescein One-Step Kit consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® & Fluorescein One-Step Kit contains premixed fluorescein for optional use as a passive reference.

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