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产品名/货号/品牌 简介 包装  
Cas9 核酸酶,S. pyogenes, 50 pmol

目录价:¥

货号:M0386S
品牌:NEB

经销:基因有限公司

Cas9 核酸酶,S. pyogenes, 50 pmol Cas9 Nuclease, S. pyogenes, 50 pmol,Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Q5 Site-Directed Mutagenesis Kit/诱导位点特异性突变的Q5体系 1

目录价:¥

货号:E0552S
品牌:NEB

经销:基因有限公司

Q5? Site-Directed Mutagenesis Kit (Without Competent Cells), 10 reactions 诱导位点特异性突变的Q5体系,不含感受态细胞,能够在低于2h内实现双链质粒DNA上的定点突变,PCR完成后,可直接加入体系中的KLD(Kinase-Ligase-DpnI)室温混合5min,快速实现片段环化和模板去除。10个反应。

Cas9 核酸酶,S. pyogenes, 300 pmol

目录价:¥

货号:M0386T
品牌:NEB

经销:基因有限公司

Cas9 核酸酶,S. pyogenes  Cas9 Nuclease, S. pyogenes, 300 pmol,Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Cas9 核酸酶,S. pyogenes,250 pmol

目录价:¥

货号:M0386L
品牌:NEB

经销:基因有限公司

Cas9 核酸酶,S. pyogenes,250 pmol Cas9 Nuclease, S. pyogenes, 250 pmol,Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

EnGen™ 基因编辑突变检测试剂盒    

目录价:¥

货号:E3321S
品牌:NEB

经销:基因有限公司

EnGen™ 基因编辑突变检测试剂盒    , EnGen™ Mutation Detection Kit, 25 rxns;The EnGen Mutation Detection Kit provides reagents for detection of on-target genome editing events. In the first step, targeted regions from cells whose genomes were targeted (i.e. CRISPR/Cas9, TALENs, Zinc-finger Nucleases) are amplified using Q5 Hot Start High-Fidelity 2X Master Mix. Upon denaturation and re-annealing, heteroduplexes are formed when mutations from insertions and deletions (indels) are present in the amplicon pool. In the second step, annealed PCR products are digested with EnGen T7 Endonuclease I, a structure-specific enzyme that will recognize mismatches larger than 1 base. Both strands of the DNA are cut when a mismatch is present, which results in the formation of smaller fragments. Analysis of the resulting fragments provides an estimate of the efficiency of the genome editing experiments. The EnGen Mutation Detection Kit includes a Control Template and Primer Mix that can be used as a control for the PCR reaction and T7 Endonuclease I digestion. The Control Template and Primer Mix provided contains two plasmids and primers that when amplified, denatured and re-annealed will form heteroduplexes that contain a 10-base insertion. This structure is a substrate for T7 Endonuclease I. The digestion of the 600 bp heteroduplex containing amplicon yields products of 200 bp and 400 bp. 600 bp parental homoduplexes are uncleaved, and are easily distinguished from cleaved heteroduplexes when separated and visualized by agarose gel electrophoresis or fragment analysis instrument. The protocol has been optimized so that PCR products generated by the Q5 Hot Start High-Fidelity 2X Master Mix can be introduced directly into the T7 Endonuclease I digestion without the need for purification. Digestion of the heteroduplex is complete in only 15 minutes, and Proteinase K is included to stop the reaction efficiently. Additional Q5 Hot Start High-Fidelity 2X Master Mix is also included to allow for optimization of target site amplification before digestion.

Cas9 核酸酶,S. pyogenes,500 pmol

目录价:¥

货号:M0386M
品牌:NEB

经销:基因有限公司

Cas9 核酸酶,S. pyogenes,500 pmol Cas9 Nuclease, S. pyogenes, high conc., 500 pmol,Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Mut Express® II Fast Mutagenesis Kit 10 rxn

目录价:¥

货号:C212-01
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® II Fast Mutagenesis Kit是基于ClonExpress®快速克隆技术的定点突变统。使用本试剂盒,目标质粒扩增产物经DpnI消化、ClonExpress®重组环化后直接进行转化即可完成定点突变。该试剂盒由Phanta® Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块组成。Phanta® Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突变的可能性。高度优化的

Mut Express® II Fast Mutagenesis Kit V2 10 rxn

目录价:¥

货号:C214-01
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® II Fast Mutagenesis Kit V2是基于ClonExpress®快速克隆技术的定点突变统。使用本试剂盒,目标质粒扩增产物经DpnI消化、ClonExpress®重组环化后直接进行转化即可完成定点突变。该试剂盒由Phanta® Max Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块组成。Phanta® Max Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突

Mut Express® II Fast Mutagenesis Kit 25 rxn

目录价:¥

货号:C212-02
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® II Fast Mutagenesis Kit是基于ClonExpress®快速克隆技术的定点突变统。使用本试剂盒,目标质粒扩增产物经DpnI消化、ClonExpress®重组环化后直接进行转化即可完成定点突变。该试剂盒由Phanta® Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块组成。Phanta® Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突变的可能性。高度优化的

Mut Express® II Fast Mutagenesis Kit V2 25 rxn

目录价:¥

货号:C214-02
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® II Fast Mutagenesis Kit V2是基于ClonExpress®快速克隆技术的定点突变统。使用本试剂盒,目标质粒扩增产物经DpnI消化、ClonExpress®重组环化后直接进行转化即可完成定点突变。该试剂盒由Phanta® Max Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块组成。Phanta® Max Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突

Mut Express® MultiS Fast Mutagenesis Kit 10 rxn

目录价:¥

货号:C213-01
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® MultiS Fast Mutagenesis Kit可一次性向目标质粒上三至五个不连续位点同时引入定点突变。该试剂盒由两个模块组成:Phanta® Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块。Phanta® Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突变的可能性。使用Mut Express® MultiS定点突变试剂盒进行DNA多点突变时,引物设计灵活,模板使用量

Mut Express® MultiS Fast Mutagenesis Kit V2 10 rxn

目录价:¥

货号:C215-01
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® MultiS Fast Mutagenesis Kit V2是Mut Express® MultiS Fast Mutagenesis Kit的升级版本。扩增模块由Phanta® Super-Fidelity DNA Polymerase升级为Phanta® Max Super-Fidelity DNA Polymerase,扩增能力更强、模板兼容性更广且高GC片段扩增成功率显著提高。此外,试剂盒优化了引物设计方式:由“每对正反向引物5’端包含至少20 bp反向互补区域”修改为“

Mut Express® MultiS Fast Mutagenesis Kit 25 rxn

目录价:¥

货号:C213-02
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® MultiS Fast Mutagenesis Kit可一次性向目标质粒上三至五个不连续位点同时引入定点突变。该试剂盒由两个模块组成:Phanta® Super-Fidelity DNA Polymerase扩增模块和ClonExpress®快速克隆模块。Phanta® Super-Fidelity DNA Polymerase超高的保真度显著降低了扩增过程中引入新突变的可能性。使用Mut Express® MultiS定点突变试剂盒进行DNA多点突变时,引物设计灵活,模板使用量

Mut Express® MultiS Fast Mutagenesis Kit V2 25 rxn

目录价:¥

货号:C215-02
品牌:Vazyme

经销:南京诺唯赞生物

Mut Express® MultiS Fast Mutagenesis Kit V2是Mut Express® MultiS Fast Mutagenesis Kit的升级版本。扩增模块由Phanta® Super-Fidelity DNA Polymerase升级为Phanta® Max Super-Fidelity DNA Polymerase,扩增能力更强、模板兼容性更广且高GC片段扩增成功率显著提高。此外,试剂盒优化了引物设计方式:由“每对正反向引物5’端包含至少20 bp反向互补区域”修改为“

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