TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mLBlocking buffer and antibody diluent for Western blotting applications that contains Santa Cruz Biotechnology Immunoanalytical Grade Blottocontains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid formmakes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2OTBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B: sc-2335 which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20

TBS Blotto B 特别推荐用于磷酸化抗体， 以冻干粉的形式装在一次性使用的瓶中，重悬至终体积500 mL封闭试剂，以及一抗和二抗的稀释液，可减少免疫印迹应用中的非特异结合和背景；优于BSA免疫分析级别的封闭液包含了1%奶粉， 1%封闭试剂， 0.05%吐温-20； 吐温-20以液态形式单独装在一个瓶子里用去离子水重悬至500ml终体积，制备1X溶液为抑制丝氨酸/苏氨酸磷酸酶活性， 可添加50mM 氟化钠 (NaF)（sc-24988）到封闭液中.及/或 Sodium Orthovanadate (Na3O4V)(sc-3540)抑制蛋白质酪氨酸磷酸酶活性。另一种选择， 可以添加磷酸酶抑制剂A(sc-45044)和磷酸酶抑制剂B(sc-45045)推荐用于降低总体的背景，减少与使用HRP标记的一抗、二抗，或者小鼠IgG结合蛋白的增强化学发光（ECL）蛋白检测法相关的非特异信号。对于近红外光谱（NIR）检测法， 我们强烈推荐替代产品，UltraCruz® 封闭试剂 (sc-516214)如果不是用磷酸化抗体做免疫印迹，我们推荐替代产品，TBS Blotto A (sc-2333)，它包含5%奶粉，0.05% 吐温-20的独特配方

Blotto, non-fat dry milk is supplied as a lyophilized powder in a single-use bottle; available in 2 sizes: 250 g and 500 gRecommended to prepare both our TBS Blotto A: sc-2333 and TBS Blotto B: sc-2335 blocking solutionsBlotto, non-fat dry milk is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214

100 ml ready-to-use solution for blocking and dilutionPlant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.animal-free alternative to sera, BSA or non-fat dry milkDesigned to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applicationsThe UltraCruz® Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are usedHighly recommended for use with Near-Infrared (NIR) Western blot detection methods, such as LI-COR/Odyssey® and other comparable systems