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生物通商城首页 -> 试剂 > 免疫学 > Western Blot免疫印迹 > 封闭剂

免疫学

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二抗及Ig结合蛋白

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Western Blot免疫印迹

免疫沉淀

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ELISPOT

AlphaLISA

AlphaScreen/互作分析

LANCE(TR-FRET)

多重细胞因子检测

DELFIA TRF检测

流式

同型对照

免疫对照/血清/阻断/封闭

诊断相关血清

封闭剂

免疫缓冲剂/固定/透化

细胞裂解/组织抽提液

染色前处理/Mount

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产品名/货号/品牌 简介 包装  
TBS Blotto A:TBS Blotto A 1 bottle

价格:¥

货号:sc-2333
品牌:Santa Cruz

经销:基因有限公司

TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mLBlocking buffer and antibody diluent for Western blotting applications that contains Santa Cruz Biotechnology Immunoanalytical Grade Blottocontains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid formmakes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2OTBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B: sc-2335 which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20

TBS Blotto B:TBS Blotto B 1 bottle

价格:¥

货号:sc-2335
品牌:Santa Cruz

经销:基因有限公司

TBS Blotto B 特别推荐用于磷酸化抗体, 以冻干粉的形式装在一次性使用的瓶中,重悬至终体积500 mL封闭试剂,以及一抗和二抗的稀释液,可减少免疫印迹应用中的非特异结合和背景;优于BSA免疫分析级别的封闭液包含了1%奶粉, 1%封闭试剂, 0.05%吐温-20; 吐温-20以液态形式单独装在一个瓶子里用去离子水重悬至500ml终体积,制备1X溶液为抑制丝氨酸/苏氨酸磷酸酶活性, 可添加50mM 氟化钠 (NaF)(sc-24988)到封闭液中.及/或 Sodium Orthovanadate (Na3O4V)(sc-3540)抑制蛋白质酪氨酸磷酸酶活性。另一种选择, 可以添加磷酸酶抑制剂A(sc-45044)和磷酸酶抑制剂B(sc-45045)推荐用于降低总体的背景,减少与使用HRP标记的一抗、二抗,或者小鼠IgG结合蛋白的增强化学发光(ECL)蛋白检测法相关的非特异信号。对于近红外光谱(NIR)检测法, 我们强烈推荐替代产品,UltraCruz® 封闭试剂 (sc-516214)如果不是用磷酸化抗体做免疫印迹,我们推荐替代产品,TBS Blotto A (sc-2333),它包含5%奶粉,0.05% 吐温-20的独特配方

Blotto, non-fat dry milk:Blotto, non-fat dry milk 250 g

价格:¥

货号:sc-2324
品牌:Santa Cruz

经销:基因有限公司

Blotto, non-fat dry milk is supplied as a lyophilized powder in a single-use bottle; available in 2 sizes: 250 g and 500 gRecommended to prepare both our TBS Blotto A: sc-2333 and TBS Blotto B: sc-2335 blocking solutionsBlotto, non-fat dry milk is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214

脱脂奶粉:Blotto, non-fat dry milk 500 g

价格:¥

货号:sc-2325
品牌:Santa Cruz

经销:基因有限公司

UltraCruz Blocking Reagent:UltraCruz? Blocking Reagent, 100ml 1Unit

价格:¥

货号:sc-516214
品牌:Santa Cruz

经销:基因有限公司

100 ml ready-to-use solution for blocking and dilutionPlant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.animal-free alternative to sera, BSA or non-fat dry milkDesigned to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applicationsThe UltraCruz® Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are usedHighly recommended for use with Near-Infrared (NIR) Western blot detection methods, such as LI-COR/Odyssey® and other comparable systems

Animal-Free 封闭液(5X):Animal-Free Blocking Solution (5X) 25ml

价格:¥

货号:15019S
品牌:CST

经销:基因有限公司

Animal-Free 封闭液 (5X):Animal-Free Blocking Solution (5X) 250ml

价格:¥

货号:15019L
品牌:CST

经销:基因有限公司

BSA 50g

价格:¥

货号:9998S
品牌:CST

经销:基因有限公司

牛血清蛋白BSA,50g
BSA, 50g

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