TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mLBlocking buffer and antibody diluent for Western blotting applications that contains Santa Cruz Biotechnology Immunoanalytical Grade Blottocontains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid formmakes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2OTBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B: sc-2335 which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20
Blotto, non-fat dry milk is supplied as a lyophilized powder in a single-use bottle; available in 2 sizes: 250 g and 500 gRecommended to prepare both our TBS Blotto A: sc-2333 and TBS Blotto B: sc-2335 blocking solutionsBlotto, non-fat dry milk is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214
100 ml ready-to-use solution for blocking and dilutionPlant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.animal-free alternative to sera, BSA or non-fat dry milkDesigned to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applicationsThe UltraCruz® Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are usedHighly recommended for use with Near-Infrared (NIR) Western blot detection methods, such as LI-COR/Odyssey® and other comparable systems