100 µg蛋白溶解于200 µl SDS-PAGE缓冲液免疫印迹阴性对照的细胞裂解液来自于未经转染的293细胞适合与我们的293转染细胞裂解液一起使用推荐使用：10-20 µl每泳道应储存在-20° C，尽量避免反复冻融样品管应在使用前煮沸一次

100 µg蛋白溶解于200 µl SDS-PAGE缓冲液免疫印迹阴性对照的细胞裂解液来自于未经转染的293T细胞适合与我们的293T转染细胞裂解液一起使用推荐使用：10-20 µl每泳道应储存在-20° C，尽量避免反复冻融样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferrat whole cell lysate; Raf-1 transformed fibroblast cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting buffermouse whole cell lysate; pre-adipose embryo fibroblast cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferrat whole cell lysate; thoracic aorta myoblast cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; melanoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; malignant melanoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

2 mg protein in 800 µl RIPA Lysis Bufferhuman whole cell lysate; epidermoid carcinoma cellswhole cell lysate provided for immunoprecipitation; for subsequent WB use, resuspend pellet in 80-800 µl electrophoresis sample buffer and load up to 20 µl per lane.should be stored at -80°C and repeated freezing and thawing should be minimized

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cells treated with alkaline phosphatase (AP)whole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cells induced with Calyculin Awhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

2 mg protein in 800 µl RIPA Lysis Bufferhuman whole cell lysate; epidermoid carcinoma cells induced with EGFwhole cell lysate provided for immunoprecipitation; for subsequent WB use, resuspend pellet in 80-800 µl electrophoresis sample buffer and load up to 20 µl per lane.should be stored at -80°C and repeated freezing and thawing should be minimized

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cells induced with EGFwhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cells treated with pervanadatewhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cells induced with UVwhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; epidermoid carcinoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; lung carcinoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; rhabdomyosarcoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; monocytic leukemia cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; endometrial adenocarcinoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting buffermouse whole cell lysate; smooth muscle like brain tumor cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; B cell lymphoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; breast carcinoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting buffermouse whole cell lysate; muscle myoblast cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; amelanotic melanoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting buffermouse whole cell lysate; contact sensitive embryo cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting buffermouse whole cell lysate; epithelial hepatoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; adenocarcinoma, colorectal cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; kidney adenocarcinoma cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; skin fibroblast cells induced with PDGFwhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次

500 µg protein in 200 µl SDS-PAGE Western blotting bufferhuman whole cell lysate; skin fibroblast cellswhole cell lysate provided as Western blotting positive controlshould be stored at -20°C and repeated freezing and thawing should be minimized样品管应在使用前煮沸一次