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产品名/货号/品牌 简介 包装  
Competent Cells, Bronze Efficiency, 2ml ( 10 x 200ul) 2ml

价格:¥

货号:BIO-85025
品牌:Bioline

经销:基因有限公司

感受态细胞2ml(10x200ul),试剂盒中同时提供对照载体pUC19(100pg/ul,100ul),转化用细胞,用于质粒克隆,蓝白斑筛选 High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning. 10x 200ul. α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.pUC19 DNA is also provided as a positive control.

Competent Cells, Silver Efficiency, 2ml ( 10 x 200ul ) 2ml

价格:¥

货号:BIO-85026
品牌:Bioline

经销:基因有限公司

感受态细胞2ml(10x200ul),试剂盒中同时提供对照载体pUC19(10pg/ul,100ul),转化用细胞,用于困难质粒构建和平端连接,蓝白斑筛选 High efficiency -available in ≥108 cfu/μg, perfect for difficult clones. 10x 200ul, ideal for difficult clone construction and blunt-end ligation. Reduced recombination of cloned DNA -designed to accommodates larger plasmids. Easy screening -with blue/white color selection. Convenient size -small aliquots reduce waste. α-Select Silver Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for difficult clone construction and blunt end ligation using plasmid-derived vectors. α-Select Silver Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids. pUC19 DNA is also provided as a positive control. Notes Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-

Competent Cells, Gold Efficiency, 1ml ( 20 x 50ul ) 1ml

价格:¥

货号:BIO-85027
品牌:Bioline

经销:基因有限公司

感受态细胞1ml(20x50ul),试剂盒中同时提供对照载体pUC19(10pg/ul,100ul),转化用细胞,用于困难质粒构建和平端连接,蓝白斑筛选 High efficiency - available in ≥109 cfu/μg, perfect for library construction with limited amounts of DNA. 20x50ul. Reduced recombination of cloned DNA - accommodates larger plasmids. Easy screening - with blue/white color selection. Convenient size - small aliquots reduce waste. α-Select Gold Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. α-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids. pUC19 DNA is also provided as a positive control.

DH5α 化学感受态细胞 100 μl x10

价格:¥

货号:C502-02
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌DH5α菌株制备的化学转化超级感受态细胞,转化效率超过108cfu/μg,广泛适用于质粒转化、基因克隆、蓝白斑筛选等用途。

BL21(DE3)pLysS化学感受态细胞 100 μl x10

价格:¥

货号:C505-02
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌BL21(DE3)pLysS菌株制备的化学转化超级感受态细胞,转化效率超过107cfu/μg。适于用T7启动子系统(如pET系列)表达毒性蛋白。基因型 F-ompThsdS(rB- mB-)gal dcm(DE3) [pLysS Camr]

BL21(DE3)化学感受态细胞 100 μl x10

价格:¥

货号:C504-02
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌BL21(DE3)菌株制备的化学转化超级感受态细胞,转化效率超过107cfu/μg。适用于T7启动子系统的表达载体(如pET系列),基因型 F-ompThsdS(rB-mB-)galdcm (DE3)。

XL10化学感受态细胞 100 μl x10

价格:¥

货号:C503-02
品牌:Vazyme

经销:南京诺唯赞生物

本品为克隆用化学感受态细胞使用改良Inoue法制备,增强大片段DNA转化效率,并使宿主菌细胞生长速度大幅度提高。转化效率超过108 cfu/μg,广泛适用于质粒转化、基因克隆、蓝白斑筛选等用途。

DH5α 化学感受态细胞 100 μl x20

价格:¥

货号:C502-03
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌DH5α菌株制备的化学转化超级感受态细胞,转化效率超过108cfu/μg,广泛适用于质粒转化、基因克隆、蓝白斑筛选等用途。

BL21(DE3)pLysS化学感受态细胞 100 μl x20

价格:¥

货号:C505-03
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌BL21(DE3)pLysS菌株制备的化学转化超级感受态细胞,转化效率超过107cfu/μg。适于用T7启动子系统(如pET系列)表达毒性蛋白。基因型 F-ompThsdS(rB- mB-)gal dcm(DE3) [pLysS Camr]

BL21(DE3)化学感受态细胞 100 μl x20

价格:¥

货号:C504-03
品牌:Vazyme

经销:南京诺唯赞生物

本品为使用大肠杆菌BL21(DE3)菌株制备的化学转化超级感受态细胞,转化效率超过107cfu/μg。适用于T7启动子系统的表达载体(如pET系列),基因型 F-ompThsdS(rB-mB-)galdcm (DE3)。

XL10化学感受态细胞 100 μl x20

价格:¥

货号:C503-03
品牌:Vazyme

经销:南京诺唯赞生物

本品为克隆用化学感受态细胞使用改良Inoue法制备,增强大片段DNA转化效率,并使宿主菌细胞生长速度大幅度提高。转化效率超过108 cfu/μg,广泛适用于质粒转化、基因克隆、蓝白斑筛选等用途。

BioBlue, 109, 1ml (20 x 50ul) 1ml

价格:¥

货号:BIO-85037
品牌:Bioline

经销:基因有限公司

BIOBlue Chemically Competent Cells provide an ideal host for optimal preparation of both high-quality plasmid and Lambda phage vectors. It is an excellent host for M13 and related filamentous phage and supports blue/white plaque screening and phage production.Versitile -F’ episome eliminates the need to select on minimal media plates. High efficiency - available in ≥109 cfu/μg, perfect for phage library construction. Easy screening - with blue/white color selection. Convenient size - small aliquots reduce waste.

BIOBlue Chemically Competent Cells provide an ideal host for optimal preparation of both high-quality plasmid and Lambda phage vectors. The BIOBlue strain allows blue/white color screening through α-complementation of the ß-galactosidase gene. The endA1 phenotype allows production of high-quality plasmid DNA. Single-stranded DNA can be produced from plasmids containing a phage f1 origin..

BIOBlue is also an excellent host for M13 and related filamentous phage and supports blue/white plaque screening and phage production. Maintenance of the F’ episome in this strain is facilitated via selection with tetracycline, unlike strains such as JM101 which require growth on minimal media. Genotype: recA1, endA1 gyrA96 thi-1 hsdR17(rk-, mk+) supE44 relA1 lac [F’ proAB lacIqZΔM15 Tn10(Tetr)]

CH3-Blue 109, 1ml (20 x 50ul) 1ml

价格:¥

货号:BIO-85040
品牌:Bioline

经销:基因有限公司

Provided at a transformation efficiency of 1x109 cfu/µg supercoiled DNA, making them ideal for the construction of cDNA libraries using plasmid derived vectors. Versitile -cloning of methylated and non-methylated DNA. High efficiency -available in ≥109 cfu/μg, perfect for library construction. Easy screening -with blue/white color selection. Convenient size -small aliquotes reduce waste.

To facilitate the cloning of DNA that contains methylcytosine or 5-hydroxymethylcytosine, CH3-Blue lacks the E.coli restriction systems mcrA, mcrBC, mrr and hsdRMS.

The addition of endA1 allows for cleaner preparations of DNA and better results in downstream applications. The elimination of non-specific digestion by Endonuclease I and the recA1 reduces the occurrence of non-specific recombination in cloned DNA. This minimizes recombination events and improves the quality and yield of plasmid DNA.

The lacZ mutation allows blue/white color screening and α-complementation of recombinants.

Genotype: F- ΔmcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 ara Δ139 Δ(ara, leu)7697 galU galrpsL(StrR) nupG λ-

Competent Cells, Electrocompetent, 1ml ( 10 x 100ul ) 1ml

价格:¥

货号:BIO-85028
品牌:Bioline

经销:基因有限公司

感受态细胞1ml(10x100ul),用于产生高效cDNA文库,转化效率高,可用蓝白斑筛进行简单筛选 High efficiency - available in ≥109 cfu/μg, perfect for library construction. α-Select Electrocompetent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. α-Select Electrocompetent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.pUC19 DNA is also provided as a positive control.

Electro-SHOX, 1ml (10 x 100ul) 1ml

价格:¥

货号:BIO-85038
品牌:Bioline

经销:基因有限公司

High efficiency -available in ≥1010 cfu/μg, perfect for libraries or cloning with limited amounts of DNA. Stable inserts -recA1 and endA1 markers to minimize recombination events, Versatile -cloning of methylated and non-methylated DNA. Convenient size -small aliquots reduce waste. ElectroSHOX Competent Cells are highly efficient E. coli and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as the construction of cDNA or genomic libraries or in transformations with limited input DNA.

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