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产品名/货号/品牌 简介 包装  
ProtoScript cDNA 第一链合成试剂盒:ProtoScript First Strand cDNA Synthesis Kit 30 rxns

价格:¥

货号:E6300S
品牌:NEB

经销:基因有限公司

重组的M-MuLV反转录酶,降低了RNase H活性且提高热稳定性,活性温度高达50 ℃,特异性高、cDNA产量高。可合成至少13 kb长度的cDNA。包含10X ProtoScript Ⅱ酶混合液,2X ProtoScript Ⅱ反应混合液,随机引物混合液(60μM,含1mM dNTP)、Oligo d(T)23VN 引物(含1mM dNTP,50 μM)、无核酸酶污染的水. 酶混合液含 ProtoScriptⅡ 反转录酶和小鼠 RNase 抑制剂;反应混合液含dNTPs与经过优化的缓冲液。

AMV cDNA 第一链合成试剂盒:AMV First Strand cDNA Synthesis Kit 30 rxns

价格:¥

货号:E6550S
品牌:NEB

经销:基因有限公司

AMV cDNA 第一链合成试剂盒含有 AMV 酶混合液与 AMV 2X 反应混合液。AMV 酶混合液是 AMV 反转录酶与小鼠 RNase 抑制剂的优化混合。使用 AMV 反转录酶合成 cDNA 第一链时,最佳反应温度范围广,可以从 37℃ 到 50℃。小鼠 RNase 抑制剂比人 RNase 抑制剂更耐氧化,因而能更好地保护 RNA 模板。AMV 2X 反应混合液是含有 dNTPs 的优化缓冲液。在 1X AMV 反应混合液中,AMV 酶混合液能够以高产量合成长 cDNA(达 10
AMV酶混合液是AMV反转录酶与小鼠RNase抑制剂的优化混合。最佳反应温度范围广,可以从 37℃ 到50℃。小鼠 RNase 抑制剂比人 RNase 抑制剂更耐氧化,因而能更好地保护 RNA 模板。AMV 2X 反应混合液是含有 dNTPs 的优化缓冲液。AMV 酶混合液能够以高产量合成10 kb的长cDNA。含10X AMV 酶混合液,AMV 2X反应缓冲混合液,随机引物混合液(60 μM)、Oligo d(T)23VN 引物 (50 μM)、无核酸酶污染的水

ProtoScript cDNA 第一链合成试剂盒:ProtoScript First Strand cDNA Synthesis Kit 150 rxns

价格:¥

货号:E6300L
品牌:NEB

经销:基因有限公司

重组的M-MuLV反转录酶,降低了RNase H活性且提高热稳定性,活性温度高达50 ℃,特异性高、cDNA产量高。可合成至少13 kb长度的cDNA。包含10X ProtoScript Ⅱ酶混合液,2X ProtoScript Ⅱ反应混合液,随机引物混合液(60μM,含1mM dNTP)、Oligo d(T)23VN 引物(含1mM dNTP,50 μM)、无核酸酶污染的水. 酶混合液含 ProtoScriptⅡ 反转录酶和小鼠 RNase 抑制剂;反应混合液含dNTPs与经过优化的缓冲液。

Tetro cDNA合成试剂盒:Tetro cDNA Synthesis Kit 30 Reactions

价格:¥

货号:BIO-65042
品牌:Bioline

经销:基因有限公司

cDNA合成试剂盒(30次),将RNA逆转录成cDNA,试剂盒中含酶、buffer、随机引物、Oligo dT引物、dNTP等反应所需组分。起始模板量可以低至10pg总RNA,适用于长模板和稀有模板的逆转录,模板范围10pg-2ug cDNA合成试剂盒,灵敏度高适用于从10pg到2ug的启始RNA,长基因或者低丰度模板均可胜任。 Contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable reverse transcriptase -for long genes and rare transcripts
Simple -one-tube, one-step set-up for highly sensitive PCR
Broad dynamic range -10pg to 2μg of RNA

Applications
Construction of cDNA libraries
2-step PCR assays
Generation of probes for hybridization
Gene cloning

Description
The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA.

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

SensiFAST cDNA快速合成试剂盒:SensiFAST cDNA Synthesis Kit 50 Reactions

价格:¥

货号:BIO-65053
品牌:Bioline

经销:基因有限公司

cDNA快速合成试剂盒(50次),将RNA逆转录成cDNA,试剂盒中含逆转录酶、TransAmp buffer等反应所需组分,配制反应体系更方便快捷,减少污染风险。起始模板量可以低至1pg总RNA,适用于低拷贝模板扩增,RNA全长均一扩增性能卓越 第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
Broad dynamic range – ideal for dilute and low-copy samples
Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
Applications
Two-step RT-PCR
Two-step RT-qPCR

Description
SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

Tetro cDNA合成试剂盒:Tetro cDNA Synthesis Kit 100 Reactions

价格:¥

货号:BIO-65043
品牌:Bioline

经销:基因有限公司

cDNA合成试剂盒(100次),将RNA逆转录成cDNA,试剂盒中含酶、buffer、随机引物、Oligo dT引物、dNTP等反应所需组分。起始模板量可以低至10pg总RNA,适用于长模板和稀有模板的逆转录,模板范围10pg-2ug cDNA合成试剂盒,灵敏度高适用于从10pg到2ug的启始RNA,长基因或者低丰度模板均可胜任。 Contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
Ultra-stable reverse transcriptase -for long genes and rare transcripts
Simple -one-tube, one-step set-up for highly sensitive PCR
Broad dynamic range -10pg to 2μg of RNA

Applications
Construction of cDNA libraries
2-step PCR assays
Generation of probes for hybridization
Gene cloning

Description
The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA.

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

SensiFAST cDNA快速合成试剂盒:SensiFAST cDNA Synthesis Kit 250 Reactions

价格:¥

货号:BIO-65054
品牌:Bioline

经销:基因有限公司

cDNA快速合成试剂盒(250次),将RNA逆转录成cDNA,试剂盒中含逆转录酶、TransAmp buffer等反应所需组分,配制反应体系更方便快捷,减少污染风险。起始模板量可以低至1pg总RNA,适用于低拷贝模板扩增,RNA全长均一扩增性能卓越 第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
Broad dynamic range – ideal for dilute and low-copy samples
Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
Applications
Two-step RT-PCR
Two-step RT-qPCR

Description
SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

SensiFAST cDNA Synthesis Kit, sample size 1

价格:¥

货号:BIO-65053/S
品牌:Bioline

经销:基因有限公司

第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
Broad dynamic range – ideal for dilute and low-copy samples
Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
Applications
Two-step RT-PCR
Two-step RT-qPCR

Description
SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

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