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SNAP-Surface 阻断剂 :SNAP-Surface Block 200 nmol

价格:¥

货号:S9143S
品牌:NEB

经销:基因有限公司

阻断剂是非荧光底物,在活细胞表面(SNAP-Surface Block)阻断 SNAP-tag 的反应。在 SNAP-tag 融合蛋白的活细胞标记实验中,可以用阻断剂制备无荧光对照。SNAP-Surface 阻断剂也与 SNAP-tag 不可逆反应,使之在随后的标记步骤中失活。该阻断剂不能透过细胞膜,仅可阻断暴露于细胞表面的 SNAP-tag。

pSNAPf 载体:pSNAPf Vector 20 μg

价格:¥

货号:N9183S
品牌:NEB

经销:基因有限公司

在哺乳动物细胞稳定和瞬时表达。 哺乳动物SNAPf载体表达各种SNAP-tags 的速度更快。tag 表达载体的上、下游具有改良后的多克隆位点,使得 tag 可以表达在目的蛋白的任何一端,该表达过程受 CMV 启动子调控。SNAPf-tag表达载体含有一个新霉素抗性基因(NeoR),可用于筛选稳定转染子。载体上还带有一个 IRES 元件,有助于融合蛋白和 NeoR 的高效表达。

pSNAP-tag(T7)-2 载体:pSNAP-tag(T7)-2 Vector 20 μg

价格:¥

货号:N9181S
品牌:NEB

经销:基因有限公司

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl chloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates.

SNAP-Surface 488 50 nmol

价格:¥

货号:S9124S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光506 nm,发射光526nm。SNAP-Surface® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate (BG-488) is based on ATTO-TEC dye ATTO 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 506 nm and emission maximum at 526 nm. This package includes 50 nmol of SNAP-Surface 488, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface 549 50 nmol

价格:¥

货号:S9112S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光560nm,发射光575nm。SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate (BG-549) is based on the Dyomics dye DY-549P1 and is suitable for use with standard TAMRA or Cy3 filter sets. It has an excitation maximum at 560 nm and emission maximum at 575 nm. This package includes 50 nmol of SNAP-Surface 549, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface 594 50 nmol

价格:¥

货号:S9134S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光606nm,发射光626 nm。SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells. This substrate (BG-594) is based on the ATTO-TEC dye ATTO 594 and is suitable for standard Texas Red and Alexa 594 filter sets. It has an excitation maximum at 606 nm and emission maximum at 626 nm. This package includes 50 nmol of SNAP-Surface 594 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on manmmalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface 649 50 nmol

价格:¥

货号:S9159S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发波长655nm,发射波长676nm。SNAP-Surface 649 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins on cell surfaces and in solution. This substrate (BG-649) is based on the Dyomics dye DY-649P1 and is suitable for excitation with a 650 nm diode laser or use with Cy5 filter sets. It has an excitation maximum at 655 nm and emission maximum at 676 nm. This package includes 50 nmol of SNAP-Surface 649 substrate, sufficient to make 10 ml of a 5 μM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine- DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAPtag substrate is described below.

SNAP-Surface Alexa Fluor® 488:SNAP-Surface Alexa Fluor 488 50 nmol

价格:¥

货号:S9129S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光496nm,发射光520nm。SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on BG-Alexa Fluor 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 496 nm and emission maximum at 520 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 488 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface Alexa Fluor 546 50 nmol

价格:¥

货号:S9132S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光 558nm,发射光574nm。SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on Alexa Fluor 546 and is suitable for standard TAMRA or Cy3 filter sets. It has an excitation maximum at 558 nm and emission maximum at 574 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 546 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface Alexa Fluor 647 50 nmol

价格:¥

货号:S9136S
品牌:NEB

经销:基因有限公司

细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光652nm,发射光670nm。SNAP-Surface Alexa Fluor 647 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on Alexa Fluor 647 and is suitable for standard 635 nm or 650 nm diode laser excitation. It has an excitation maximum at 652 nm and emission maximum at 670 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 647 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

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