收藏生物通商城 您好!欢迎来到生物通商城![请登录],新用户?[免费注册] 立即咨询 QQ 点击这里给我发消息 我的订单 帮助

商品比较

抗体搜索:
生物通商城首页 -> 试剂 -> 分子生物学 -> 核酸纯化 -> 总RNA/DNA/蛋白同步分离

分子生物学

工具酶

核酸纯化

PCR

RT-PCR

定量PCR/RT-PCR产品

转染/转化/转导

文库构建

组装克隆和筛选

表达系统

突变检测和转座子系统

测序产品

电泳

荧光原位杂交

RNAi

miRNA

RNA分析

杂交膜/杂交系统

cDNA合成

报告系统/蛋白标记定位

细菌培养

基因敲除

基因分型

基因组DNA

培养基

单细胞分析

噬菌体肽库展示

  • 促销专题
  • 您浏览过的产品

清除历史

商品筛选

特性:
不限FFPE RNA/DNA纯化RNA/DNA纯化
品牌:
不限 MNG Epicentre Bioline
抗体筛选:
筛选:
简介:
  • 排序
  • 价格
  • 关注度
  • 销量
  • 更新时间
  • 第1 / 1页 共9条 上一页 下一页
产品名/货号/品牌 简介 包装  
NucleoSpin RNA/Protein (10) 10

价格:¥

货号:740933.10
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin TriPrep (10) 10

价格:¥

货号:740966.10
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin RNA/DNA Buffer Set buffer set for separated isolation of RNA and DNA from one lysate, only applicable with NucleoSpin RNA II, NucleoSpin RNA XS, NucleoSpin miRNA, NS RNA Blood, NucleoSpin RNA Plant, or NucleoSpin RNA/Protein kits, sufficient fo 100

价格:¥

货号:740944
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA and DNA from undivided samples in one working procedure
• To be used in combination with NucleoSpin® RNA II, NucleoSpin® RNA XS, NucleoSpin® miRNA, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein kits
• No need to split samples (e. g., precious, indivisible samples like biopsy material)
• High quality DNA and RNA from one sample, perfect for PCR, RT-PCR, real-time PCR
• Fast procedure

Format Buffer set for elution of DNA in combination with RNA preparations, to be used in combination with NucleoSpin® RNA kits
DNA size < 30 kbp
Typical DNA yield 5 µg from 106 HeLa cells; 16 µg from 30 mg pig liver; 5 µg from 100 mg maize leaf
A260/A280 1.7–2.0
DNA elution volume 100 µL
Applications*
• For biopsy material: RNA for expression analysis; DNA for mutation analysis (e.g., of cancer genes)
• For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (e.g., TBE-V, Tick-borne encephalitis virus, infectious agent of meningoencephalitis); DNA for analysis of germs (e.g., Borrelia burgdorferi, infective agent of the Lyme disease)
• For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or other genes; DNA for analysis of integration site, integration number and sequence confirmation of the transformed gene
• For transfected cultured cells: RNA for expression analysis of the transgene or other genes of interest; DNA for analysis of methylation status of the transgene or other genes of interest


NucleoSpin RNA/Protein (50) 50

价格:¥

货号:740933.50
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin TriPrep (50) 50

价格:¥

货号:740966.50
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin RNA/Protein (250) 250

价格:¥

货号:740933.250
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA and protein from undivided samples
• Reliable interpretation of RNA and protein extracted from the same sample→ direct correlation – no splitting of samples required
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for DNA-free RNA of high quality

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the isolation of RNA and protein from one lysate - NucleoSpin RNA/Protein Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin TriPrep (250) 250

价格:¥

货号:740966.250
品牌:MNG

经销:基因有限公司

Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: ≤ 5 x 10(6) cells; ≤ 30 mg human/animal tissue; ≤ 100 mg plant tissue
Total RNA: Fragment size > 200 b; Typical yield < 70 µg; A260/A280 1.9–2.1; Typical RIN (RNA integrity number) > 9; Elution volume 40–120 µL; Binding capacity 200 µg
Total DNA: Fragment size < 30 kbp; A260/A280 1.7–1.9; Typical yield < 6 µg; Elution volume 100 µL; Binding capacity 10 µg
Total protein: Fragment size 15–300 kDa; Typical yield < 1200 µg; Resolubilization volume 10–100 µL
Preparation time 30-35 min/6 preps
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

ISOLATE II RNA/DNA/Protein Kit, 50 preps

价格:¥

货号:BIO-52085
品牌:Bioline

经销:基因有限公司

30分钟内从单个样品中依次分离总RNA(含小分子RNA),基因组DNA,和总蛋白质,适用于组织,细胞,血液和其他体液,微生物等。 For the sequential isolation of high quality total RNA (including small RNA), genomic DNA and total protein from a single sample without splitting the lysate, for highly reliable analysis of the transcriptome, genome and proteome

•Rapid, phenol-free workflow - reliably purify RNA/DNA/Proteins from a single sample in ≤30 mins
•Novel separation matrix - superior yields of high quality molecules
•Complete diversity of RNA - all sizes of RNA recovered from mRNA to miRNA/siRNA
•Purify small samples - sequential isolation ideal for precious or small biopsy material
•DNase I included for complete removal of genomic DNA in RNA purification

Applications Isolation of total RNA (including small RNA)/DNA/Protein from:
•Mammalian tissue
•Eukaryotic cell lines
•Bacteria, yeast, fungi
•Blood and other biofluids
Pure, isolated molecules ready for all downstream applications such as real-time RT-PCR, RT-PCR, RNA-seq, genotyping, 2D gel electrophoresis, Western blotting, SDS-PAGE and Mass Spectrometry.

Description
ISOLATE II RNA/DNA/Protein Kit is a specially developed 3-in-1 kit that provides a rapid method for the sequential isolation and purification of high quality total RNA (including small RNA), genomic DNA and protein from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants.

ISOLATE II RNA/DNA/Protein Kit is ideal for the isolation of macromolecules from precious or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis becomes more reliable since the RNA, DNA and proteins are derived from the same sample (i.e. purified molecules are from the same population of cells being studied).

ISOLATE II RNA/DNA/Protein Kit allows convenient processing of multiple samples in 30 minutes, utilizing a two-column system. Genomic DNA is bound to the first column while the total RNA and proteins are removed in the flow-through. The bound DNA is then washed and highly purified DNA is eluted. The flow-through is then loaded onto the second column, where total RNA is bound while the proteins are removed in the flow-through. The bound total RNA is washed and purified RNA is eluted (fig. 1 and 2). Proteins are rapidly purified and eluted using the column.

ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and can be used in a wide variety of downstream applications including expression profiling, miRNA profiling, siRNA gene silencing studies, novel biomarker discovery, epigenetics, genotyping, transgenic analysis and cell line characterization.

Components Reagent 50 Reactions
ISOLATE II RNA/Protein Columns 50
ISOLATE II DNA Columns 50
Elution Tubes (1.7ml) 150
Elution Tubes (1.7ml) 150
Lysis Buffer TX 40ml
Wash Buffer W1 (Concentrate) 2 x 38ml
Wash Buffer W2 (Concentrate) 15ml
Wash Buffer W3 30ml
DNase I Reaction Buffer DRB 6ml
DNase I Solution (RNase-free) 0.8ml
RNA Elution Buffer 6ml
DNA Elution Buffer 6ml
Protein Elution Buffer 8ml
Protein Binding Buffer 8ml
Protein Neutralization Buffer 4ml
Protein Loading Dye 2ml
Product Manual 1
Bench Protocol Sheet 1

Storage & StabilityThe DNase I Solution should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label. Customer Testimonials "This kit (ISOLATE II RNA/DNA/Protein) means we would require less plates of cells, less steps in dividing up a sample (such as tissue) and most importantly time in sample prep." Michael Epis, The Harry Perkins Institute of Medical Research

ISOLATE II FFPE RNA/DNA Kit , 50 preps 50 preps

价格:¥

货号:BIO-52087
品牌:Bioline

经销:基因有限公司

从福尔马林固定石蜡包埋样品中高效率纯化总RNA和小分子RNA(包括miRNA),基因组DNA(>80bp),单次样品最多可达10mg石蜡块或者多至4张切片(<20um厚),RNA硅胶模纯化柱载量35ug, DNA纯化柱载量10ug。操作简单快捷。For the isolation of high yield, pure total RNA, including small RNA and genomic DNA from formalin-fixed paraffin embedded (FFPE) tissue samples.

•Reliable - RNA/DNA from a single FFPE sample with no splitting of the lysate, reducing variability and inconsistent results
•Isolate diverse RNA and DNA species - all sizes of RNA from large mRNA down to miRNA and all DNA above 80bp
•Reversal of formalin crosslinks - optimized for high nucleic acid yield and purity
•Phenol-free - all RNA including small RNA purified without bias
•DNase I included - complete removal of genomic DNA from RNA fraction

Applications: The purified RNA can be used in a number of downstream applications including:
•Real-Time RT-PCR
•RNA-seq
•Expression microarray

The purified genomic DNA can be used in:
•Real-Time PCR
•Multiplex PCR
•Methylation analyses by bisulfite conversion and sequencing
•Next generation sequencing
•SNP microarray
Description
ISOLATE II FFPE RNA/DNA Kit is specially designed for the rapid, phenol-free, sequential isolation of pure total RNA (including small RNA) and genomic DNA from challenging FFPE tissue samples. Formalin fixation of tissues results in crosslinking of the nucleic acids and proteins. Furthermore, the embedding process can also lead to fragmented nucleic acids with time, often resulting in lower molecular weight nucleic acids than those obtained from fresh or frozen samples. ISOLATE II FFPE RNA/DNA Kit has been developed to allow for partial reversing of formalin modifications resulting in high quality and yield of nucleic acids.

The recovery of total RNA and genomic DNA from the same sample provides the distinct advantage of providing matched nucleic acid fractions, from the same population of cells. Matched RNA/DNA is invaluable for integrative studies of the genome, epigenome and transcriptome. Maximizing RNA and DNA retrieval from a single specimen is also crucial when using tissues that are of limited availability.

Isolation is based on a fast spin column format using a novel affinity resin as the separation matrix. The RNA and DNA are preferentially purified from other cellular components without the use of phenol or chloroform, resulting in a high quality and yield of nucleic acids. The ISOLATE II FFPE RNA/DNA Kit also overcomes the bias seen in recovery of the small RNA fraction observed with phenol-based RNA recovery.

The purified RNA can be used in a number of downstream applications including real-time RT-PCR, microarrays and RNA-seq. The purified genomic DNA can be used in real-time PCR, next-generation sequencing, multiplex PCR and bisulfite conversion for epigenetic analysis.

第1 / 1页 共9条 上一页 下一页

生物通 版权所有

未经书面授权,所有页面内容、图片和设计不得以任何形式进行复制

Copyright © 2000-2017 eBiotrade.com, All Rights Reserved

本栏目业务联系电话:广州 020-87511980

主编信箱:

粤公网安备 44010602000434号 粤ICP备09063491号 免责声明

47ms47ms109ms344ms964ms