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属性:
不限CD14单核细胞CD4 T细胞NK细胞单核细胞淋巴细胞培养基树突状细胞造血细胞培养基
规格/特征:
不限无血清/化学限定
来源:
不限外周血
品牌:
不限 Lonza
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产品名/货号/品牌 简介 包装  
LGM-3 Growth Medium 500 ml 500ml

价格:¥

货号:CC-3211
品牌:Lonza

经销:基因有限公司

Lymphocyte Growth Medium is a chemically defined, serum-free, xeno-free media developed to support (with the addition of appropriate cytokines) the growth and support of human lymphocytes and dendritic cells. LGM-3™ Lymphocyte Growth Medium does not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. LGM-3™ Lymphocyte Growth Medium is devoid of any protein kinase C stimulators and is suitable for the investigation of second messenger systems in the activation of human lymphocytes. LGM-3™ Lymphocyte Growth Medium's formulation is complete and contains human albumin, recombinant human insulin, and pasteurized human transferrin. LGM-3™ Lymphocyte Growth Medium may be further supplemented with additional cytokines and growth factors depending on applications.LGM-3™ medium comes complete with albumin, insulin, transferrin, and gentamicin as the antimicrobial agent.

HPGM Hematopoietic Growth Medium, 500 ml 500ml

价格:¥

货号:PT-3926
品牌:Lonza

经销:基因有限公司

Hematopoietic Growth Medium is a chemically defined, serum-free, xeno-free media developed to support (with the addition of appropriate cytokines) the proliferation and differentiation of CD34+ hematopoietic stem cells (HSC) and hematopoietic progenitor cells (HPC). HPGM™ Hematopoietic Growth Medium does not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. HPGM™ Hematopoietic Growth Medium's formulation is complete and contains human albumin, recombinant human insulin, and pasteurized human transferrin. HPGM™ Hematopoietic Growth Medium may be further supplemented with additional cytokines and growth factors depending on applications. HPGM™ Hematopoietic Growth Medium has been successfully used in the culturing of CD34+ cells from a variety of sources including bone marrow, cord blood, peripheral blood, and fetal liver and a variety of species including both mouse and humans. HPGM™ Hematopoietic Growth Medium has been successfully used in the differentiation of CD34+ cells down a variety of lineages including erythroid and megakaryocyte.

HPBMC Peripheral Blood mononuclear cells, cryo amp 1

价格:¥

货号:CC-2702
品牌:Lonza

经销:基因有限公司

Peripheral blood mononuclear cells (PBMC) are blood cells containing a rounded nucleus (including macrophages, monocytes, and lymphocytes). Lonza peripheral blood mononuclear cells (PBMC) are isolated from peripheral blood by apheresis and density gradient centrifugation. Human Peripheral Blood Mononuclear Cells (PBMCs) are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: LGM-3™ Lymphocyte Growth Medium for serum-free culturing (additional cytokines may be necessary depending on application)

Peripheral blood CD4+ T Cells 1

价格:¥

货号:2W-200
品牌:Lonza

经销:基因有限公司

Human CD4+T Cells are a type of lymphocyte that plays an important role in regulating the cell-mediated immune response to infection. Often known as "helper" T cells, they act on other cells of the immune system to promote various aspects of the immune response, including immunoglobulin isotype switching and affinity maturation of the antibody response, macrophage activation and enhanced activity of natural killer cells and cytotoxic T cells. Poietics™ CD4+T Cells are isolated from normal peripheral blood mononuclear cells using immunomagnetic selection of the CD4 antigen. Purity is ≥ 90 %. Human CD4+ T Cells are guaranteed to express CD4 and contain ≥10 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Recommended Medium: LGM-3™ Lymphocyte Growth Medium (additional cytokines may be necessary depending on application)

NHDC Dendritic Cells cryo amp 1

价格:¥

货号:CC-2701
品牌:Lonza

经销:基因有限公司

Poietics™ Normal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of tumor necrosis factor (TNS). All dendritic cells are isolated from peripheral blood monocytes isolated by elutriation using the Elutra cell separation system and are quality tested for optimal performance. Lonza's Normal Human Dendritic Cells would typically be classified as Mo-DC/MDDC (monocyte derived dendritic cells) or CD11c+ myeloid Dendritic Cells (DC). Normal Human Dendritic Cells (NHDC) are guaranteed to contain ≥3 million viable cells per ampoule, express CD11c, and not express CD14. Testing for expression of CD80, CD86, and HLA-DR is also provided for information only. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. Recommended Medium: LGM-3™ Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Peripheral Blood NK Cells Positive 1

价格:¥

货号:2W-502
品牌:Lonza

经销:基因有限公司

Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation. Since they are part of innate immunity, they do not require sensitization for the expression of their activity. Natural Killer (NK) cells play significant roles in viral infections, autoimmunity, pregnancy, cancer, bone marrow transplantation, and more recently adaptive immunity. Natural Killer (NK) cells are most traditionally characterized by the presence of surface marker CD56, and absence of CD3. Poietics™ Human Natural Killer (NK) cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker. Purity is ≥90% based on the presence of CD56. Negative selection of Natural Killer (NK) cells results in significantly greater enrichment of CD16+ cells (usually >80% of total cells), than does positive selection which has much greater variability for CD16+ cells.
Cryopreserved Poietics™ Human Natural Killer (NK) Cells are guaranteed to express CD56 and CD16 and contain ≥5 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended Medium: LGM-3™ Lymphocyte Growth Medium (additional cytokines may be necessary depending on application)

Peripheral. Blood CD14+ Monocytes Cells 10 million 1

价格:¥

货号:2W-400C
品牌:Lonza

经销:基因有限公司

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the mononuclear cells. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability. Monocytes are isolated from the peripheral blood of screened, healthy donors. The donors are untreated. Peripheral blood is collected from the donors using apheresis. The cell product, enriched for mononuclear cells, is further processed by density centrifugation to remove the remaining red cells and neutrophils. Monocytes are then isolated using positive immunomagnetic selection directed against CD14. Human CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.
Recommended Medium: Recommended media varies based on application To Maintain the Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional) To Produce Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF To Produce Dendritic Cells: LGM-3™ Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4 Media recommendations for macrophage and dendritic cell differentiation are recommendations only. Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Peripheral Blood NK Cells Negative 1

价格:¥

货号:2W-501
品牌:Lonza

经销:基因有限公司

Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation. Since they are part of innate immunity, they do not require sensitization for the expression of their activity. Natural Killer (NK) cells play significant roles in viral infections, autoimmunity, pregnancy, cancer, bone marrow transplantation, and more recently adaptive immunity. Natural Killer (NK) cells are most traditionally characterized by the presence of surface marker CD56, and absence of CD3. Poietics™ Human Natural Killer (NK) cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker. Purity is ≥90% based on the presence of CD56. Negative selection of Natural Killer (NK) cells results in significantly greater enrichment of CD16+ cells (usually >80% of total cells), than does positive selection which has much greater variability for CD16+ cells.
Cryopreserved Poietics™ Human Natural Killer (NK) Cells are guaranteed to express CD56 and CD16 and contain ≥5 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended Medium: LGM-3™ Lymphocyte Growth Medium (additional cytokines may be necessary depending on application)

Peripheral. Blood CD14+ Monocytes Cells 20 million 1

价格:¥

货号:2W-400B
品牌:Lonza

经销:基因有限公司

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the mononuclear cells. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability. Monocytes are isolated from the peripheral blood of screened, healthy donors. The donors are untreated. Peripheral blood is collected from the donors using apheresis. The cell product, enriched for mononuclear cells, is further processed by density centrifugation to remove the remaining red cells and neutrophils. Monocytes are then isolated using positive immunomagnetic selection directed against CD14. Human CD14+ Monocytes are guaranteed to express CD14 and will contain ≥20 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.
Recommended Medium: Recommended media varies based on application To Maintain the Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional) To Produce Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF To Produce Dendritic Cells: LGM-3™ Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4 Media recommendations for macrophage and dendritic cell differentiation are recommendations only. Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Peripheral. Blood CD14+ Monocytes Cells 40 million 1

价格:¥

货号:2W-400A
品牌:Lonza

经销:基因有限公司

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the mononuclear cells. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability. Monocytes are isolated from the peripheral blood of screened, healthy donors. The donors are untreated. Peripheral blood is collected from the donors using apheresis. The cell product, enriched for mononuclear cells, is further processed by density centrifugation to remove the remaining red cells and neutrophils. Monocytes are then isolated using positive immunomagnetic selection directed against CD14. Human CD14+ Monocytes are guaranteed to express CD14 and will contain ≥40 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.
Recommended Medium: Recommended media varies based on application To Maintain the Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional) To Produce Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF To Produce Dendritic Cells: LGM-3™ Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4 Media recommendations for macrophage and dendritic cell differentiation are recommendations only. Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

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