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属性:
不限FBS 替代HL-1贴壁细胞培养基通用培养基
规格/特征:
不限不含L-谷氨酰胺含L-谷氨酰胺化学限定无血清无血清/化学限定
来源:
不限
品牌:
不限 Lonza
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产品名/货号/品牌 简介 包装  
UltraMEM Reduced Serum Medium w/ ITES 500 ml 500ml

¥

货号:12-743F
品牌:Lonza

经销:基因有限公司

without L-Gln. chemically defined media designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations (see table). When supplemented with 2 – 4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages. In addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all. Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, selenium, and ethanolamine (ITS or ITES) to the basal medium [Catalog Nos 17-838Z and 17-839Z respectively]. UltraMEM™ Reduced Serum Media are offered as a protein-free basal medium without L-glutamine, or as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/ml.

UltraMEM Reduced Serum Medium w/o L-Gln 500 ml 500ml

¥

货号:12-745F
品牌:Lonza

经销:基因有限公司

with ITES with L-Gln. chemically defined media designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations (see table). When supplemented with 2 – 4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages. In addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all. Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, selenium, and ethanolamine (ITS or ITES) to the basal medium [Catalog Nos 17-838Z and 17-839Z respectively]. UltraMEM™ Reduced Serum Media are offered as a protein-free basal medium without L-glutamine, or as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/ml. Applications – Growth and maintenance of anchorage-dependent cell types

UltraCULTURE Serum-free Medium w/o L-Gln 500ml 500ml

¥

货号:12-725F
品牌:Lonza

经销:基因有限公司

A complete, allpurpose medium designed for the cultivation of a wide variety of adherent and non-adherent mammalian cell types. UltraCULTURE™ Medium can be used to support fusion of cells during hybridoma formation, growth of monocyte, macrophage, epithelial, and fibroblastic cell lines, and generation of virus particles for vaccine production. The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-132A) to cryopreserve cells in a serum-free environment. UltraCULTURE™ Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior to use.
The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to crossreference the Master File may be obtained by contacting the Regulatory Affairs Department.
Applications
– Cultivation of adherent and non-adherent mammalian cells
– Generation of viral particles for vaccine production
Performance and Quality Tests
– Turbidity may develop in UltraCULTURE™ Medium; experiments have determined that the turbidity will not alter the performance of the product

HL-1 chemically defined FBS Substitute 10 ml 10ml

¥

货号:77227
品牌:Lonza

经销:基因有限公司

Chemically Defined FBS Substitute (100X) is a chemically defined medium additive that can be used to replace serum or significantly reduce its concentration in a variety of basal media. It contains less than 30 μg protein/ml when diluted 1:100 in medium and it does not contain bovine serum albumin or other undefined protein ingredients.HL-1™ Supplement, when added to most basal media, will support the growth of mouse hybridoma cells and certain other differentiated cells of lymphoid origin.

HL-1 Chemically Defined, Serum-free Medium for hybridoma and hematopoietic cells, without L-glutamine 2x500ml

¥

货号:77201
品牌:Lonza

经销:基因有限公司

a chemically defined culture medium containing less than 30 µg protein per ml. Components of HL-1 include water for injection, a modified DMEM/F12 base, HEPES Buffer, known amounts of insulin, transferrin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids and proprietary stabilizing proteins. It contains no bovine serum albumin or other undefined protein mixtures. HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. HL-1™ does not contain L-glutamine. Applications– Serum-free growth of hybridomas and differentiated cells of lymphoid origin

PC-1 Chemically Defined, Serum-free Medium for primary adherent cells, without L-glutamine 2x500 ml, 2x344018; 2x344022 2x500ml

¥

货号:77232
品牌:Lonza

经销:基因有限公司

a low-protein medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1™ Medium contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ Medium is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content. Applications– Cultivation of primary and anchorage-dependent cells

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