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不限ABC转运模型/膜ABC转运模型/膜对照ABC转运模型/囊泡ABC转运模型/囊泡对照ABC转运模型/囊泡分析试剂盒ATP酶分析试剂盒SLC转运系统分析试剂盒
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含ABC转运蛋白膜(对照用) / Gentest™ Control Membrane Preparation for ABC Transporters 1 Unit

价格:¥

货号:453200
品牌:Gentest

经销:基因有限公司

含重组ABC(ATP-bindingcasette transporter)转运蛋白的阴性对照,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 This preparation should be used as a negative control with recombinant ABC transporters, e.g. human P-gp (MDR1) membranes. Protein concentration: 5 mg/ml. 0.5ml

Gentest™ 肝细胞转运蛋白分析试剂盒/Gentest™ Hepatocyte Transporter Suspension Assay Kit 1 Unit

价格:¥

货号:454460
品牌:Gentest

经销:基因有限公司

肝细胞转运蛋白分析试剂盒,含有抑制转运蛋白膜活性成分,100tests,包含操作说明书、10ml滤油,100个离心管。储存过程中可能会出现分层现象,可通过低速轻甩混匀后重新使用。 This kit provides a detailed protocol for hepatocyte transporter uptake assays. It contains the critical components for stopping transport activity and separating hepatocytes from the uptake solution for 100 assay data points. This kit is a convenient in vitro method for measuring transporter activity in cryopreserved or fresh hepatocyte suspensions (Current Drug Metabolism 8:341 [2007]). The filtration assay depends on an oil layer or “cushion” which serves as a barrier between the reaction mix (hepatocytes and substrate) and a lower layer of 2N NaOH. After the incubation period, the assay mixture (containing cells, substrate, and buffer) is layered on top of the oil cushion and centrifuged briefly. The hepatocytes pass through the filtration oil into the lower layer of NaOH, which serves to solubilize the cell membrane and release the radiolabeled substrate.
The oil layer barrier prevents the aqueous assay mix containing free radiolabeled substrate from entering the NaOH layer.The lower portion of the microcentrifuge tube, containing the cells and transported substrate, is cut away from the upper layer, mixed with scintillation cocktail, and measured by scintillation counting. The assay is carried out at both 4°C and 37°C; the 4°C sample serves as the negative control (disintegrations per minute [DPM] at 4°C represents nonspecific binding).

大鼠P-糖蛋白结合的ABC膜/大鼠Mdr1a膜/Gentest™ Rat P-gp (Mdr1a) Membrane 1 Unit

价格:¥

货号:453440
品牌:Gentest

经销:基因有限公司

含大鼠P-糖蛋白结合的ABC家族Mdr1b蛋白的人工膜,一种ATP依赖的物质转运泵,在肠道、大脑、肝脏等组织中内源表达,可实现跨膜转运药物的功能,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Rat Mdr1a is an ATP-dependent efflux pump capable of transporting many drugs across cell membranes. It is expressed in the intestine, brain, liver, and other tissues. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform a colorimetric ATPase assay in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative verapamil-stimulated vanadate-sensitive ATPase activity: xx* nmol phosphate/(mg membrane protein)(min). Protein concentration: 5 mg/ml. Package size: 0.5 ml.

小鼠ABC蛋白超家族Mdr1b蛋白膜/Gentest™ Mouse P-gp (Mdr1b) Membranes 1 Unit

价格:¥

货号:453250
品牌:Gentest

经销:基因有限公司

含小鼠ABC蛋白超家族Mdr1b蛋白的人工膜,一种ATP依赖的物质转运泵,可实现跨膜转运药物的功能,在小肠、大脑、肝脏等组织中内源表达,可作为肿瘤化学药物治疗效果的动物实验指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 Mouse Mdr1b is an ATP-dependent efflux pump capable of transporting many drugs across cell membranes. It is expressed in the intestine, liver, and other tissues. 0.5mL, 5mg/mL.
This product is prepared from baculovirus-infected insect cells. A method to perform a colorimetric ATPase assay in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative verapamil-stimulated vanadate-sensitive ATPase activity: xx* nmol phosphate/(mg membrane protein)(min). Protein concentration: 5 mg/ml. Package size: 0.5 ml.

大鼠P-糖蛋白结合的ABC膜/大鼠Mdr1b膜/Gentest™ Rat P-gp (Mdr1b) Membranes 1 Unit

价格:¥

货号:453434
品牌:Gentest

经销:基因有限公司

含大鼠P-糖蛋白结合的ABC家族Mdr1b蛋白的人工膜,一种ATP依赖的物质转运泵,在肠道、肝脏等组织中内源表达,可实现跨膜转运药物的功能,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Rat MDR1b is an ATP-dependent efflux pump capable of transporting many drugs across cell membranes. It is expressed in the intestine, liver, and other tissues. 0.5mL, 5mg/mL.
This product is prepared from baculovirus-infected insect cells. A method to perform a colorimetric ATPase assay in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative verapamil-stimulated vanadate-sensitive ATPase activity: 7 nmol phosphate/(mg membrane protein)(min). Protien concentration: 5 mg/ml. Package size: 0.5 ml.

人P-糖蛋白结合的ABC膜/人MDR1膜/Gentest™ Human P-gp (MDR1) Membranes 1 Unit

价格:¥

货号:453228
品牌:Gentest

经销:基因有限公司

含人P-糖蛋白结合的ABC家族MDR1蛋白的人工膜,一种ATP依赖的物质转运泵,可实现跨膜转运药物的功能,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Human P-gp (MDR1) is an ATP-dependent efflux pump that is capable of transporting many drugs across cell membranes. High level expression of this protein has been linked to tumor resistance to chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus-infected insect cells. A method to perform a colorimetric ATPase assay in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative verapamil-stimulated, vanadate-sensitive ATPase activity: 32 nmol phosphate/(mg membrane protein)(min).

小鼠ABC蛋白超家族Mdr1a蛋白膜/Gentest™ Mouse (Mdr1a) Membranes 1 Unit

价格:¥

货号:453232
品牌:Gentest

经销:基因有限公司

含小鼠ABC蛋白超家族Mdr1a蛋白膜的人工膜,一种ATP依赖的物质转运泵,可实现跨膜转运药物的功能,在小肠、大脑、肝脏等组织中内源表达,可作为肿瘤化学药物治疗效果的动物实验指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 Mouse MDR1a is an ATP-dependent efflux pump capable of transporting many drugs across cell membranes. It is expressed in the intestine, brain, liver, and other tissues. 0.5mL, 5mg/mL.
This product is prepared from baculovirus-infected insect cells. A method to perform a colorimetric ATPase assay in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative verapamil-stimulated vanadate-sensitive ATPase activity: 14 nmol phosphate/(mg membrane protein)(min). Protien concentration: 5 mg/ml. Package size: 0.5 ml.

大鼠BRCP膜/Gentest™ Rat BRCP Membranes 1 Unit

价格:¥

货号:453457
品牌:Gentest

经销:基因有限公司

大鼠BRCP膜,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 BCRP (MXR/ABCG2) is a member of ABC superfamily that expresses in many tissues, such as brain, placenta, liver, and intestine. BCRP plays important roles in protecting those tissues against toxic compounds, drugs, and drug metabolites. Rat BCRP was cloned from rat liver cDNA library. 0.5mL, 5mg/mL.
This product was prepared using baculovirus infected insect cells expression system. A method to perform a colorimetric ATPase assay in 96 well plates is provided in the batch data sheet. Other applications of this product are possible. Representative PhIP-stimulated vanadate-sensitive ATPase activity: 33 nmol phosphate/(mg membrane protein)(min).

人ABC蛋白超家族成员MRP3膜/Gentest™ Human MRP3 Membranes 1 Unit

价格:¥

货号:453333
品牌:Gentest

经销:基因有限公司

含人ABC蛋白超家族成员MRP3蛋白的人工膜,在肝脏、肾上腺、小肠等组织中内源表达,参与有机阴离子盐、有机阴离子偶联化合物和其他ATPase底物的转运,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Human MRP3 is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in liver, adrenal glands, kidney, small intestine, and other tissues. Human MRP3 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform a colorimetric ATPase assay in 96 well plates is provided in the batch data sheet. Other applications of this product are possible. Representative Benzbromarone-stimulated vanadate-sensitive ATPase activity: 10.8 nmol phosphate/(mg membrane protein)(min).

人ABC蛋白超家族成员MRP1膜/Gentest™ Human MRP1 Membranes 1 Unit

价格:¥

货号:453456
品牌:Gentest

经销:基因有限公司

含人ABC蛋白超家族成员MRP1蛋白的人工膜,在肺、睾丸、骨骼肌等组织中内源表达,参与有机阴离子盐、有机阴离子偶联化合物和其他ATPase底物的转运,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Human MRP1 (ABCC1) is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in lung, testis, skeletal muscle, and other tissues. Human MRP1 is capable of transporting many organic anions, organic anion conjugates and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform a colorimetric ATPase assay in 96 well plates is provided in the batch data sheet. Other applications of this product are possible. Representative NEM-stimulated vanadate-sensitive ATPase activity: xx nmol phosphate/(mg membrane protein)(min).

Gentest™ BSEP Vesicle Assay Kit Gentest™ BSEP囊泡分析试剂盒 1 Unit

价格:¥

货号:459011
品牌:Gentest

经销:基因有限公司

BSEP囊泡分析试剂盒,200 tests This assay kit characterizes the substrates or inhibitors of Bile Salt Export Pump (BSEP). The assay measures the cumulative uptake of test compounds in the inside-out BSEP vesicle transporter to directly identify BSEP substrates or inhibitors.

Shelf Life:2 years
Shipping:Dry ice
Features
• Supports EMA recommended BSEP
• Provides one simple protocol to accommodate both Corning human and animal BSEP vesicle studies
• Supports high-volume cell harvester applications with bulk wash buffers available separately
• Saves researchers time by eliminating reagent preparation steps; assays can be completed within one day
• Supports multiple time usage with robust, stable reagents accommodating up to six freeze-thaw cycles

Contents
Kit contains reagents for 200 assays
• Assay Uptake Buffer
• 10 X Wash Buffer
• 200 mM ATP
• 200 mM AMP
• BSEP Substrate
• Blocking Buffer (50X)

Preparation and Storage: -20°C

人ABC蛋白超家族成员MRP2膜//Gentest™ Human MRP2 Membranes 1 Unit

价格:¥

货号:453332
品牌:Gentest

经销:基因有限公司

含人ABC蛋白超家族成员MRP2蛋白的人工膜,在肝脏、肾脏、大脑等组织中内源表达,参与有机阴离子盐、有机阴离子偶联化合物和其他ATPase底物的跨膜转运,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 Human MRP2 an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in liver, kidney, brain, and other tissues. Human MRP2 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform a colorimetric ATPase assay in 96 well plates is provided in the batch data sheet. Other applications of this product are possible. Representative probenecid-stimulated vanadate-sensitive ATPase activity: xx nmol phosphate/(mg membrane protein)(min).

小鼠BCRP膜/Gentest™ Mouse BCRP Membranes 1 Unit

价格:¥

货号:453251
品牌:Gentest

经销:基因有限公司

小鼠BCRP膜,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 BCRP (MXR/ABCG2) is a member of the ABC superfamily. It expresses in many tissues, such as brain, placenta, liver, and intestine. BCRP plays important roles in protecting those tissues against toxic compounds, drugs, and drug metabolites. Mouse BCRP was cloned from mouse liver cDNA library. 0.5mL, 5mg/mL.
This product was prepared using baculovirus infected insect cells expression system. A method to perform a colorimetric ATPase assay in 96 well plates is provided in the batch data sheet. Other applications of this product are possible. Representative Sulfasalazine-stimulated vanadate-sensitive ATPase activity: XX nmol phosphate/(mg membrane protein)(min).

Gentest™ ABC转运蛋白囊泡(对照用)/Gentest™ Control Vesicle Preparation for ABC Transporters 1 Unit

价格:¥

货号:453500
品牌:Gentest

经销:基因有限公司

含ABC(ATP-bindingcasette transporter)转运蛋白的控制囊泡,一种重组形式提供的蛋白控制囊泡,阴性对照,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记化合物的摄取分析。 This preparation should be used as a negative control with recombinant ABC transporter vesicles. E.g.: human MRP2 Vesicles (Cat. No. 453450). Representative LTC4 Uptake Activity: 2.6 pmole/min/mg in the presence of ATP; 1.2 pmol/min/mg in the absence of ATP. 0.5mL, 5mg/mL.

大鼠ABC蛋白超家族MRP1囊泡/Gentest™ Rat MRP1 Vesicles 1 Unit

价格:¥

货号:453510
品牌:Gentest

经销:基因有限公司

含大鼠ABC蛋白超家族MRP1蛋白的囊泡,在肝脏、肾脏、大脑等组织中内源表达,参与有机阴离子盐、有机阴离子偶联化合物和其他ATPase底物的转运,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Rat MRP1 (ABCC1) is an ATP-dependent efflux pump, belonging to the ABC transporter superfamily. It is expressed in liver, kidney, brain, and other tissues. Rat MRP1 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative LTC4 Uptake Activity: 78 pmole/min/mg in the presence of ATP; 14 pmole/min/mg in the absence of ATP.

大鼠BSEP囊泡/Gentest™ Rat BSEP Vesicles 1 Unit

价格:¥

货号:453501
品牌:Gentest

经销:基因有限公司

大鼠BSEP囊泡,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Rat BSEP is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. Expressed in liver, RAT SEP is capable of transporting bile salts, some organic anions, and organic anion conjugates across hepatic canalicular membrane. 0.5mL, 5mg/mL. This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative TCA Uptake Activity: 3.2 pmole/min/mg in the presence of ATP; 1.3 pmole/min/mg in the absence of ATP.

大鼠ABC蛋白超家族MRP2囊泡/Gentest™ Rat MRP2 Vesicles 1 Unit

价格:¥

货号:453523
品牌:Gentest

经销:基因有限公司

含大鼠ABC蛋白超家族MRP2蛋白的囊泡,在肝脏、肾脏、大脑等组织中内源表达,参与有机阴离子盐、有机阴离子偶联化合物和其他ATPase底物的转运,可作为肿瘤化学药物治疗效果的指标,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Rat MRP2 (ABCC2) is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in liver, kidney, brain, and other tissues. Rat MRP2 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative LTC4 Uptake Activity: X.X pmole/min/mg in the presence of ATP; X.X pmole/min/mg in the absence of ATP.

人ABC蛋白超家族成员MRP1囊泡/Gentest™ Human MRP1 Vesicles 1 Unit

价格:¥

货号:453455
品牌:Gentest

经销:基因有限公司

含人P-糖蛋白结合的ABC家族MRP1蛋白的囊泡,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Human MRP1 (ABCC1) is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in lung, testis, skeletal muscle, and other tissues. Human MRP1 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative LTC4 Uptake Activity: 70 pmole/min/mg in the presence of ATP; 13 pmole/min/mg in the absence of ATP.

人ABC蛋白超家族成员MRP2囊泡/Gentest™ Human MRP2 Vesicles 1 Unit

价格:¥

货号:453450
品牌:Gentest

经销:基因有限公司

含人P-糖蛋白结合的ABC家族MRP2蛋白的囊泡,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Human MRP2 (ABCC2) is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in liver, kidney, brain, and other tissues. Human MRP2 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL.
This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative LTC4 Uptake Activity: 7.4 pmole/min/mg in the presence of ATP; 0.9 pmole/min/mg in the absence of ATP.

人ABC蛋白超家族成员MRP3囊泡/Gentest™ Human MRP3 Vesicles 1 Unit

价格:¥

货号:453520
品牌:Gentest

经销:基因有限公司

含人P-糖蛋白结合的ABC家族MRP3蛋白的囊泡,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Human MRP3 (ABCC3) is an ATP-dependent efflux pump belonging to the ABC transporter superfamily. It is expressed in adrenal glands, kidney, small intestine, and other tissues. Human MRP3 is capable of transporting many organic anions, organic anion conjugates, and other substrates across cell membranes. High level expression of this protein has been linked to drug resistance during chemotherapy. 0.5mL, 5mg/mL 。 This product is prepared from baculovirus infected insect cells. A method to perform an uptake assay with and without ATP using radiolabeled substrates in 96-well plates is provided in the batch data sheet. Other applications of this product are possible. Representative E217bG Uptake Activity: 2.2 pmole/min/mg in the presence of ATP; 0.5 pmole/min/mg in the absence of ATP.

人BSEP囊泡模型/Gentest™ Human BSEP Vesicles 1 Unit

价格:¥

货号:453502
品牌:Gentest

经销:基因有限公司

人BSEP囊泡模型,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Human BSEP Vesicles, 0.5mL, 5mg/mL

人 BCRP (Thr482) 膜模型/Gentest™ Human BCRP (Thr482) Membranes 1 Unit

价格:¥

货号:453257
品牌:Gentest

经销:基因有限公司

人 BCRP (Thr482) 膜,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Human BCRP (Thr482) Membranes, 0.5mL, 5mg/mL

人BCRP (Arg482)膜模型/Gentest™ Human BCRP (Arg482) Membranes 1 Unit

价格:¥

货号:453270
品牌:Gentest

经销:基因有限公司

人BCRP (Arg482)膜模型,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性 Human BCRP (Arg482) Membranes, 0.5mL, 5mg/mL

Gentest™ MRP/BCRP Vesicle Assay KitGentest™ MRP/BCRP 囊泡分析试剂盒 1 Unit

价格:¥

货号:459010
品牌:Gentest

经销:基因有限公司

MRP/BCRP 囊泡分析试剂盒,200 tests MRP/BCRP Vesicle Assay Kit for characterizing the substrates or inhibitors of MRP/BCRP transporters. The assay measures the cumulative uptake of test compounds in the inside-out MRP or BCRP vesicle transporters to directly determine the substrates or inhibitors of ABC transporters. This is done using radioisotope-labeled compounds or fluorescence compounds. The kit provides reagents for 200 assays and accommodates 4 vials of vesicles.

Features
• Supports FDA-recommended BCRP, as well as MRP vesicle panel.
• Provides one simple protocol to accommodate Corning human and animal MRP/BCRP vesicle studies.
• Delivers maximum flexibility allowing researchers the ability to perform a single transporter study or both MRP and BCRP studies with one kit.
• Supports high-volume cell harvester applications with bulk wash buffers available separately.
• Provides researchers an alternative to radiolabeled compounds with included CDCF fluorescent substrate for MRP2/MRP3 transporters.
• Saves researchers time by eliminating reagent preparation steps; assays can be completed within one day.
• Supports multiple time usage with robust, stable reagents accommodating up to six freeze-thaw cycles.

Contents
Kit contains reagents for 200 assays
• Assay Uptake Buffer
• 10X Wash Buffer
• 200 mM ATP
• 200 mM AMP
• 300 mM GSH
• MPR3 Substrate
• BCRP Substrate
• MRP2/MPR3 Fluorescent Substrate
• MRP1/MRP2 Substrate

Preparation and Storage:-80°C
Shelf Life:2 years
Shipping:Dry ice

大鼠BCRP囊泡/Gentest™ Rat BCRP Vesicles 1 Unit

价格:¥

货号:453345
品牌:Gentest

经销:基因有限公司

含大鼠BCRP蛋白的囊泡,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Rat BCRP Vesicles, 0.5mL, 5mg/mL

人BCRP (Arg482)囊泡模型/Gentest™ Human BCRP (Arg482) Vesicles 1 Unit

价格:¥

货号:453271
品牌:Gentest

经销:基因有限公司

人BCRP (Arg482)囊泡模型,5mg/ml,0.5ml。操作简便,可用于放射性元素标记或未标记药物化合物的摄取分析,适用于高通量分析。 Human BCRP (Arg482) Vesicles, 0.5mL, 5mg/mL

小鼠BCRP囊泡/Gentest™ Mouse BCRP Vesicles 1 Unit

价格:¥

货号:453252
品牌:Gentest

经销:基因有限公司

小鼠BCRP囊泡,5mg/ml,0.5ml。操作简便,高通量,适用于非放射性标记的ABC蛋白底物分析,或检测药物刺激的钒酸酯敏感性ATP酶活性。 Mouse BCRP Vesicles, 0.5mL, 5mg/mL

Corning® ATP酶分析试剂盒/Corning® ATPase Assay Kit 1 Unit

价格:¥

货号:459006
品牌:Gentest

经销:基因有限公司

ATP酶分析试剂盒,5*96 次反应。比色分析法,可通过检测释放的无机盐磷的含量快速检测ABC转运蛋白状态。保质期:2年。 This colorimetric ATPase assay kit rapidly screens ABC transporter proteins (membranes) for potential substrates or inhibitors. The assay determines ATPase activity by measuring the release of inorganic phosphate resulting from substrate-stimulated ATP.

Shelf Life:2 years. Quality: Validated using P-gp, MRP1, MRP2, MRP3 and BCRP membrane

TransportoCells™ Control, 10 Million Cells 1 Unit

价格:¥

货号:354854
品牌:Gentest

经销:基因有限公司

Control. Cryopreserved cells can be thawed, plated and assayed in just two days. Robust results with uptake ratios =10 fold. Consistent with other mammalian cell-based models. One vial contains 10 million cells and supports one 24-well or one 96-well plate. Includes all current USFDA and European Medicines Agency (EMA)-recommended SLC drug transporters.

Corning TransportoCells products are high performance mammalian cells in a convenient, cryo­preserved format that transiently overexpress a single human SLC transporter protein. The frozen cells deliver robust data, while eliminating the time required to culture and maintain stable cell lines.

Corning® TransportoCells™ SLC Transporter Cells Frequently Asked Questions

1. What are Corning TransportoCells products?
Corning TransportoCells products are cryopreserved HEK293 cells transiently overexpressing human SLC drug transporter proteins. Each TransportoCells product contains an individually expressed SLC transporter.
2. Which SLC transporters are available as TransportoCells products?
The SLC transporters are OATP1B1, OATP1B3, OAT1, OAT3, OCT1 and OCT2.
3. Which SLC transporters are recommended for testing by the FDA and EMA in the most recent guidance documents published by these regulatory agencies?
The 2012 FDA Draft Guidance recommends testing OATP1B1, OATP1B3, OAT1, OAT3 and OCT2. The 2013 EMA Guidance recommends testing OATP1B1, OATP1B3, OAT1, OAT3, OCT1 and OCT2. All of these transporters are available as TransportoCells products.
4. Is a control cell available for testing along with the TransportoCells products?
Control cells, which are parent HEK293 cells transiently expressing empty expression vector, are available (Cat. No. 354854). We recommend that negative control cells be tested side­by­side with TransportoCells products.
5. How are TransportoCells products shipped and stored? Are there any special handling procedures when the product arrives at my lab?
TransportoCells products are cryopreserved cells in a 2 mL cryogenic vial. The product is shipped in a liquid nitrogen dry shipper. Upon arrival in your lab, the vials should be removed immediately from the dry shipper and placed in the vapor phase of a liquid nitrogen storage tank. The empty dry shipper should be returned following the instructions included.
6. What assays can be performed using TransportoCells products?
Typical assays that can be performed with TransportoCells products include:
Reaction phenotyping: determining if a specific SLC transporter is capable of transporting a test compound from the outside of a cell to the inside.
Kinetic assays: determining the Km and Vmax values for the uptake of a test compound.
Inhibition profiling: determining IC50 and Ki values for inhibitor/drug­drug interaction (DDI) screening.
7. What are the basic steps for preparing an uptake assay with TransportoCells products? Are detailed instructions for using TransportoCells products provided?
The steps involved in preparing TransportoCells for an assay include thawing and plating the cells on day one and performing the assay 24 hours after plating. Detailed instructions for thaw­ing and plating cells, as well as conducting an uptake assay, are provided with each shipment of TransportoCells products and are available online at www.corning.com/lifesciences.
8. How many cells can be recovered from each vial of Corning® TransportoCells™ product? How many assay plates can be prepared with one vial of cells?
One vial of TransportoCells product provides a minimum of 10 million viable cells following the thawing procedure in the “Instructions for Use.” Typically 10 to 15 million viable cells can be obtained per vial. One vial of cells are enough for one 24 well plate or one 96 well plate.
9. What is post-thaw viability of TransportoCells products?
The post­thaw viability is equal to, or greater than, 80%.
10. What is substrate-uptake performance of TransportoCells products?
The uptake performance of TransportoCells products is reported as the “uptake ratio.” The uptake ratio of the quality control substrate for each TransportoCells product is equal to, or greater than, 10. The uptake ratio is calculated by dividing the uptake activity of the quality control substrate in TransportoCells products by the uptake activity in the control cells.
11. Are the products tested for mycoplasma?
TransportoCells products are tested and found negative for mycoplasma.
12. Can antibiotics be used in the plating media?
You can use antibiotics with TransportoCells products but they are not required.
13. Is sodium butyrate recommended for use in the assay?
Sodium butyrate is recommended to boost the uptake activity of OATP1B1 and OATP1B3, but not OAT1, OAT3, OCT1 and OCT2. In­house data demonstrate that sodium butyrate has minimal effect on the uptake activity of OATs and OCTs. Addition of sodium butyrate into the cell culture can negatively impact the cell confluence.
14. What surface can be used to plate TransportoCells products?
Poly­D­Lysine coated plates are recommended for use with TransportoCells products. Other surfaces can be used, but the cell attachment, confluency and assay robustness might be compromised.
15. What assay buffer can be used with TransportoCells products other than HBSS (Ca2+Mg2+)?
Krebs­Henseleit Buffer (KHB) can be used with TransportoCells products. However, KHB buffer can easily form precipitation when warmed. It has to be made fresh each time before use. In­house data demonstrate HBSS without Ca2+Mg2+ does not perform as well as HBSS with Ca2+Mg2+.
16. Do test compounds need to be radiolableled? Are non-labeled compounds and detection by mass spectrometry possible alternatives?
Radiolabeled scintillation detection assays, non­labeled/LC­MS assays, and fluorescent detection assays have been developed using TransportoCells products. More information about using MS and fluorescent detection systems for uptake assays can be obtained by contacting Corning Technical Support at CLStechserv@corning.com.
17. For detection by mass spectrometry, does the sample require any further process, such as removal of protein prior to analysis?
The cells can be lysed with 80% acetonitrile when using mass spectrometry to facilitate protein precipitation. After centrifugation, the supernatant can be injected into an LC­MS and analyzed. More information about LC­MS analysis can be obtained by contacting Corning Technical Support at CLStechserv@corning.com.
18. How long can TransportoCells products be kept in liquid nitrogen vapor phase storage?
TransportoCells products have a 3­year shelf life.3
19. What is the uptake assay “window” (i.e., how long can the cells be maintained after plating and retain optimal uptake assay performance)?
Corning® TransportoCells™ products have been purposely optimized to have maximal uptake activity at 24 to 48 hours after plating. The short window of optimal uptake activity eliminates the need for cell culture maintenance and allows for quick return of assay results within 24 to 48 hours after thawing the cells.
20. Does the assay have to be performed exactly 24 hours after plating?
The Instructions for Use provide an optimized protocol for performing the assay at 24 hours. In­house data demonstrate that similar uptake activity can be achieved when the assays are performed between 18 hours and 30 hours after plating, but not longer.
21. Can TransportoCells products be subcultured after the assay is complete? Can a frozen cell bank be prepared for future use?
TransportoCells products are designed as a “consumable” for one time use. They are not to be further cultured beyond the “best used by” time described in the instructions, which is within 48 hours after thawing and plating. By design, TransportoCells products quickly lose uptake activity 48 hours after plating.
22. What is the organic solvent tolerance for the uptake assay using TransportoCells products? What is the recommended maximum final test compound solvent concentration?
We recommended using less than 1% of organic solvent. In­house data demonstrate that 5% DMSO can significantly impact the uptake activity of the TransportoCells products. Other organic solvents, such as methanol and acetonitrile, have not been tested.

Organic anion transporter 1,OAT1 / SLC22A6, 10 million cells 1 Unit

价格:¥

货号:354857
品牌:Gentest

经销:基因有限公司

OAT1 / SLC22A6. Cryopreserved cells can be thawed, plated and assayed in just two days. Robust results with uptake ratios =10 fold. Consistent with other mammalian cell-based models. One vial contains 10 million cells and supports one 24-well or one 96-well plate. Includes all current USFDA and European Medicines Agency (EMA)-recommended SLC drug transporters.

Corning TransportoCells products are high performance mammalian cells in a convenient, cryo­preserved format that transiently overexpress a single human SLC transporter protein. The frozen cells deliver robust data, while eliminating the time required to culture and maintain stable cell lines.

Corning® TransportoCells™ SLC Transporter Cells Frequently Asked Questions

1. What are Corning TransportoCells products?
Corning TransportoCells products are cryopreserved HEK293 cells transiently overexpressing human SLC drug transporter proteins. Each TransportoCells product contains an individually expressed SLC transporter.
2. Which SLC transporters are available as TransportoCells products?
The SLC transporters are OATP1B1, OATP1B3, OAT1, OAT3, OCT1 and OCT2.
3. Which SLC transporters are recommended for testing by the FDA and EMA in the most recent guidance documents published by these regulatory agencies?
The 2012 FDA Draft Guidance recommends testing OATP1B1, OATP1B3, OAT1, OAT3 and OCT2. The 2013 EMA Guidance recommends testing OATP1B1, OATP1B3, OAT1, OAT3, OCT1 and OCT2. All of these transporters are available as TransportoCells products.
4. Is a control cell available for testing along with the TransportoCells products?
Control cells, which are parent HEK293 cells transiently expressing empty expression vector, are available (Cat. No. 354854). We recommend that negative control cells be tested side­by­side with TransportoCells products.
5. How are TransportoCells products shipped and stored? Are there any special handling procedures when the product arrives at my lab?
TransportoCells products are cryopreserved cells in a 2 mL cryogenic vial. The product is shipped in a liquid nitrogen dry shipper. Upon arrival in your lab, the vials should be removed immediately from the dry shipper and placed in the vapor phase of a liquid nitrogen storage tank. The empty dry shipper should be returned following the instructions included.
6. What assays can be performed using TransportoCells products?
Typical assays that can be performed with TransportoCells products include:
Reaction phenotyping: determining if a specific SLC transporter is capable of transporting a test compound from the outside of a cell to the inside.
Kinetic assays: determining the Km and Vmax values for the uptake of a test compound.
Inhibition profiling: determining IC50 and Ki values for inhibitor/drug­drug interaction (DDI) screening.
7. What are the basic steps for preparing an uptake assay with TransportoCells products? Are detailed instructions for using TransportoCells products provided?
The steps involved in preparing TransportoCells for an assay include thawing and plating the cells on day one and performing the assay 24 hours after plating. Detailed instructions for thaw­ing and plating cells, as well as conducting an uptake assay, are provided with each shipment of TransportoCells products and are available online at www.corning.com/lifesciences.
8. How many cells can be recovered from each vial of Corning® TransportoCells™ product? How many assay plates can be prepared with one vial of cells?
One vial of TransportoCells product provides a minimum of 10 million viable cells following the thawing procedure in the “Instructions for Use.” Typically 10 to 15 million viable cells can be obtained per vial. One vial of cells are enough for one 24 well plate or one 96 well plate.
9. What is post-thaw viability of TransportoCells products?
The post­thaw viability is equal to, or greater than, 80%.
10. What is substrate-uptake performance of TransportoCells products?
The uptake performance of TransportoCells products is reported as the “uptake ratio.” The uptake ratio of the quality control substrate for each TransportoCells product is equal to, or greater than, 10. The uptake ratio is calculated by dividing the uptake activity of the quality control substrate in TransportoCells products by the uptake activity in the control cells.
11. Are the products tested for mycoplasma?
TransportoCells products are tested and found negative for mycoplasma.
12. Can antibiotics be used in the plating media?
You can use antibiotics with TransportoCells products but they are not required.
13. Is sodium butyrate recommended for use in the assay?
Sodium butyrate is recommended to boost the uptake activity of OATP1B1 and OATP1B3, but not OAT1, OAT3, OCT1 and OCT2. In­house data demonstrate that sodium butyrate has minimal effect on the uptake activity of OATs and OCTs. Addition of sodium butyrate into the cell culture can negatively impact the cell confluence.
14. What surface can be used to plate TransportoCells products?
Poly­D­Lysine coated plates are recommended for use with TransportoCells products. Other surfaces can be used, but the cell attachment, confluency and assay robustness might be compromised.
15. What assay buffer can be used with TransportoCells products other than HBSS (Ca2+Mg2+)?
Krebs­Henseleit Buffer (KHB) can be used with TransportoCells products. However, KHB buffer can easily form precipitation when warmed. It has to be made fresh each time before use. In­house data demonstrate HBSS without Ca2+Mg2+ does not perform as well as HBSS with Ca2+Mg2+.
16. Do test compounds need to be radiolableled? Are non-labeled compounds and detection by mass spectrometry possible alternatives?
Radiolabeled scintillation detection assays, non­labeled/LC­MS assays, and fluorescent detection assays have been developed using TransportoCells products. More information about using MS and fluorescent detection systems for uptake assays can be obtained by contacting Corning Technical Support at CLStechserv@corning.com.
17. For detection by mass spectrometry, does the sample require any further process, such as removal of protein prior to analysis?
The cells can be lysed with 80% acetonitrile when using mass spectrometry to facilitate protein precipitation. After centrifugation, the supernatant can be injected into an LC­MS and analyzed. More information about LC­MS analysis can be obtained by contacting Corning Technical Support at CLStechserv@corning.com.
18. How long can TransportoCells products be kept in liquid nitrogen vapor phase storage?
TransportoCells products have a 3­year shelf life.3
19. What is the uptake assay “window” (i.e., how long can the cells be maintained after plating and retain optimal uptake assay performance)?
Corning® TransportoCells™ products have been purposely optimized to have maximal uptake activity at 24 to 48 hours after plating. The short window of optimal uptake activity eliminates the need for cell culture maintenance and allows for quick return of assay results within 24 to 48 hours after thawing the cells.
20. Does the assay have to be performed exactly 24 hours after plating?
The Instructions for Use provide an optimized protocol for performing the assay at 24 hours. In­house data demonstrate that similar uptake activity can be achieved when the assays are performed between 18 hours and 30 hours after plating, but not longer.
21. Can TransportoCells products be subcultured after the assay is complete? Can a frozen cell bank be prepared for future use?
TransportoCells products are designed as a “consumable” for one time use. They are not to be further cultured beyond the “best used by” time described in the instructions, which is within 48 hours after thawing and plating. By design, TransportoCells products quickly lose uptake activity 48 hours after plating.
22. What is the organic solvent tolerance for the uptake assay using TransportoCells products? What is the recommended maximum final test compound solvent concentration?
We recommended using less than 1% of organic solvent. In­house data demonstrate that 5% DMSO can significantly impact the uptake activity of the TransportoCells products. Other organic solvents, such as methanol and acetonitrile, have not been tested.

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