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产品名/货号/品牌 简介 包装  
高分辨率熔解曲线试剂盒:SensiFAST HRM Kit 500 rxns

价格:¥

货号:BIO-32005
品牌:Bioline

经销:基因有限公司

高分辨率熔解曲线试剂盒(HRM,500次反应),采用最新酶化学增强剂,结合抗体介导的热启动酶和过饱和荧光染料,使得HRM分析可以更快、更特异、更灵敏,而且有很好的可重现性 SensiFAST HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis. 20ul x 500rxns

Ultra-sensitive: accurately discriminates class 4 (A/T) SNP mutations
Rapid - enabling accurate results to be achieved faster
Highly reproducible - highly-specific, high-resolution melt analysis
Optimized - assays quickly and reliably established, even for difficult genomic loci

Instrument Compatibility
ABI 7300, 7500 FAST, Qiagen Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex, Illumina® Eco™, Roche LightCycler®; 96 and 480, Bio-Rad Opticon®, CFX96™, CFX384™, Idaho LightScanner® 32 (LS32™), Takara Thermal Cycler Dice (TP800) (See product selection table).

Description
High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses.

SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis, ideal for small and medium-sized genotyping projects.

The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such as class 4 SNPs (Fig. 1) without sequence preference. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping, epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons).

高分辨率熔解曲线试剂盒:SensiFAST HRM Kit 2000 rxns

价格:¥

货号:BIO-32020
品牌:Bioline

经销:基因有限公司

SensiFAST HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis. 20ul x 2000rxns

Ultra-sensitive: accurately discriminates class 4 (A/T) SNP mutations
Rapid - enabling accurate results to be achieved faster
Highly reproducible - highly-specific, high-resolution melt analysis
Optimized - assays quickly and reliably established, even for difficult genomic loci

Instrument Compatibility
ABI 7300, 7500 FAST, Qiagen Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex, Illumina® Eco™, Roche LightCycler®; 96 and 480, Bio-Rad Opticon®, CFX96™, CFX384™, Idaho LightScanner® 32 (LS32™), Takara Thermal Cycler Dice (TP800) (See product selection table).

Description
High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses.

SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis, ideal for small and medium-sized genotyping projects.

The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such as class 4 SNPs (Fig. 1) without sequence preference. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping, epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons).

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