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DNA 提取对照试剂(Orange Cap):DNA Extraction Control 560 (Orange Cap) 500 rxns

价格:¥

货号:BIO-35031
品牌:Bioline

经销:基因有限公司

DNA Extraction Control (containing Cal Fluor® Orange 560 labeled probe) enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are spiked into the lysis buffer with the sample prior to DNA extraction. Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.

Simple - easy monitoring and validation of DNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
DNA Extraction Control 560 is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

DNA Extraction Control 560 uses Cal Fluor® Orange 560 and is also available with Quasar® 670 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after DNA extraction. This monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1). Bioline have specially developed a DNA Extraction Control (DEC), which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the DEC is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.

The DEC cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The DEC cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix (primers and probe) is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). DEC also monitors co-purification of PCR inhibitors (fig. 3) that may cause biased or false amplification patterns.

DNA 提取对照试剂(Red Cap):DNA Extraction Control 670 (Red Cap) 500 rxns

价格:¥

货号:BIO-35028
品牌:Bioline

经销:基因有限公司

DNA提取对照试剂(500ul),是一款辅助监测样本核酸提取成败的试剂,将该产品与样本混合后同时进行核酸提取及下游PCR分析以监测上游核酸提取的优劣。试剂盒中含有DNA核酸提取对照试剂、阳性序列引物及探针,适用于ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®仪器 DNA Extraction Control containing Quasar® 670 labeled probe enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are spiked into the lysis buffer with the sample prior to DNA extraction. Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.

Simple - easy monitoring and validation of DNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
DNA Extraction Control 670 is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

DNA Extraction Control 670 uses Quasar® 670 and is also available with Cal Fluor® Orange 560 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after DNA extraction. This monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1).

Bioline has specially developed a DNA Extraction Control (DEC) which more closely mimics the test sample, as compared to spike controls. The genetic material from the test sample and the DEC is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.

The DEC cells are of a known concentration and contain the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The DEC cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix (primers and probe) is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). DEC also monitors co-purification of PCR inhibitors (fig. 3) that may cause biased or false amplification patterns.

RNA 提取对照试剂(560通道):RNA Extraction Control 560 500 Reactions

价格:¥

货号:BIO-38045
品牌:Bioline

经销:基因有限公司

RNA Extraction Control enables users of diagnostic assays to validate their extraction step. RNA Extraction Control contains a known concentration of artificial cells that contain the control RNA sequence. Cells containing internal control RNA are spiked into the lysis buffer with the sample prior to RNA extraction. Following extraction, the reaction mix is added to the extracted RNA prior to reverse transcription and amplification. Presence of internal control RNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample RNA.

Simple - easy monitoring and validation of RNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
RNA Extraction Control is suitable for use with commercially available silica-membrane RNA extraction kits and CHELEX matrices and has been extensively tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

To fit in with existing protocols, RNA Extraction Control 560 uses Cal Fluor® Orange 560 and is also available with Quasar® 670 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after RNA extraction. This monitors PCR inhibition but has no value as an extraction control or indicator of reverse transcription efficiency. RNA Extraction Control (REC) provides quality control assurance for the entire workflow, from sample extraction and reverse transcription/cDNA synthesis through to real-time amplification and analysis.

The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1). Bioline has specially developed a RNA Extraction Control that more closely mimics the test sample, as compared to “spike” controls. The genetic material from the test sample and REC is simultaneously extracted through common extraction methods, with REC being as sensitive to inhibition and extraction failure as the test sample.

REC consists of artificial cells of a known concentration that contain the Internal Control RNA sequence. This sequence has no known homology to any organism and, importantly, has minimal interference with the detection of sample RNA. REC cells are “spiked” into the lysis buffer with the target sample prior to DNA extraction.

The Control Mix, comprising primers and probe, is then added to the reaction mix prior to amplification. The signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). REC also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns (fig. 3).

RNA 提取对照试剂(670通道):RNA Extraction Control 670 500 Reactions

价格:¥

货号:BIO-38041
品牌:Bioline

经销:基因有限公司

RNA Extraction Control enables users of diagnostic assays to validate their extraction step. RNA Extraction Control contains a known concentration of artificial cells that contain the control RNA sequence. Cells containing internal control RNA are spiked into the lysis buffer with the sample prior to RNA extraction. Following extraction, the reaction mix is added to the extracted RNA prior to reverse transcription and amplification. Presence of internal control RNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample RNA.

Simple - easy monitoring and validation of RNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
RNA Extraction Control is suitable for use with commercially available silica-membrane RNA extraction kits and CHELEX matrices and has been extensively tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

RNA Extraction Control 670 uses Quasar® 670 and is also available with Cal Fluor® Orange 560 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after RNA extraction. This monitors PCR inhibition but has no value as an extraction control or indicator of reverse transcription efficiency. RNA Extraction Control (REC) provides quality control assurance for the entire workflow, from sample extraction and reverse transcription/cDNA synthesis through to real-time amplification and analysis.

The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1). Bioline has specially developed a RNA Extraction Control that more closely mimics the test sample, as compared to “spike” controls. The genetic material from the test sample and REC is simultaneously extracted through common extraction methods, with REC being as sensitive to inhibition and extraction failure as the test sample.

REC consists of artificial cells of a known concentration that contain the Internal Control RNA sequence. This sequence has no known homology to any organism and, importantly, has minimal interference with the detection of sample RNA. REC cells are “spiked” into the lysis buffer with the target sample prior to DNA extraction.

The Control Mix, comprising primers and probe, is then added to the reaction mix prior to amplification. The signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). REC also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns (fig. 3).

DNA 提取对照试剂(Orange Cap):DNA Extraction Control 560 (Orange Cap) 2000 rxns

价格:¥

货号:BIO-35032
品牌:Bioline

经销:基因有限公司

DNA Extraction Control (containing Cal Fluor® Orange 560 labeled probe) enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are spiked into the lysis buffer with the sample prior to DNA extraction. Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.

Simple - easy monitoring and validation of DNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
DNA Extraction Control 560 is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

DNA Extraction Control 560 uses Cal Fluor® Orange 560 and is also available with Quasar® 670 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after DNA extraction. This monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1). Bioline have specially developed a DNA Extraction Control (DEC), which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the DEC is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.

The DEC cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The DEC cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix (primers and probe) is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). DEC also monitors co-purification of PCR inhibitors (fig. 3) that may cause biased or false amplification patterns.

DNA 提取对照试剂(Red Cap):DNA Extraction Control 670 (Red Cap) 2000 rxns

价格:¥

货号:BIO-35029
品牌:Bioline

经销:基因有限公司

DNA Extraction Control containing Quasar® 670 labeled probe enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are spiked into the lysis buffer with the sample prior to DNA extraction. Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.

Simple - easy monitoring and validation of DNA extraction protocols
Specific - minimal interference with sample detection
Optimized - ideal for blood, urine and sputum samples
Sensitive - specially designed for real-time PCR assays

Instrument Compatibility
DNA Extraction Control 670 is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

DNA Extraction Control 670 uses Quasar® 670 and is also available with Cal Fluor® Orange 560 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

Description
A common practice in real-time PCR is to add a known amount of “spiked” control DNA after DNA extraction. This monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1).

Bioline has specially developed a DNA Extraction Control (DEC) which more closely mimics the test sample, as compared to spike controls. The genetic material from the test sample and the DEC is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.

The DEC cells are of a known concentration and contain the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The DEC cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix (primers and probe) is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step (fig. 2). DEC also monitors co-purification of PCR inhibitors (fig. 3) that may cause biased or false amplification patterns.

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