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不限IPTGX-GAL
品牌:
不限 Bioline
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产品名/货号/品牌 简介 包装  
IPTG, 5g 5g

价格:¥

货号:BIO-37036
品牌:Bioline

经销:基因有限公司

IPTG,5g,蛋白表达诱导剂 IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

Induces E.coli lac operon activity - suitable for cloning and protein expression procedures
Dioxane free - does not disrupt normal cell function
>99.6% purity - confirmed by HPLC
Convenient - available as powder and stabilized stock solution

Applications
Blue/white color screening
Induction of lac operon for protein expression
Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG

Description
Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG.

X-GAL, 1g 1g

价格:¥

货号:BIO-37035
品牌:Bioline

经销:基因有限公司

X-GAL is a chromogenic substrate for ß-Galactosidase used to differentiate recombinants from nonrecombinants in cloning experiments using vectors containing the lacZ or lacZ α-peptide gene. Perfect for cloning - gives an intense blue precipitate upon hydrolysis by β-galactosidase. Extremely pure - confirmed by HPLC

Applications
Blue/White cloning systems
Immunoblotting
Immunocytochemical assays
Microbiology and cell culture media

Description
5-bromo-4-chloro-3-indolyl ß-D-galactopyranoside (X-GAL) is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate.It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect micro-organisms which have ß-Galactosidase activity (usually coliforms). It can be combined with the R-substrates to differentiate between two species of organisms on the same plate. X-GAL is soluble in N, N-dimethylformamide.

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