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几丁质珠:Chitin Resin 10 ml

价格:¥

货号:S6651V
品牌:NEB

经销:基因有限公司

一种亲和介质,能少量分离纯化与 CBD (Chitin Binding Domain)融合的目的蛋白。

几丁质珠:Chitin Resin 20 ml

价格:¥

货号:S6651S
品牌:NEB

经销:基因有限公司

一种亲和介质,能少量分离纯化与 CBD (Chitin Binding Domain)融合的目的蛋白。

Amylose 树脂:Amylose Resin 10 ml

价格:¥

货号:E8021V
品牌:NEB

经销:基因有限公司

每毫升柱床体积可结合 6-8 mg MBP5-paramyosin ΔSal 融合蛋白。

几丁质磁珠:Chitin Magnetic Beads 5 ml

价格:¥

货号:E8036S
品牌:NEB

经销:基因有限公司

一种亲和介质,能少量分离纯化与 CBD (Chitin Binding Domain)融合的目的蛋白。几丁质磁珠是直径约为 50-70 μm 的顺磁微粒。100 μl 几丁质磁珠能结合 30-50 μg CBD 融合蛋白

Amylose 树脂:Amylose Resin 15 ml

价格:¥

货号:E8021S
品牌:NEB

经销:基因有限公司

每毫升柱床体积可结合 6-8 mg MBP5-paramyosin ΔSal 融合蛋白。

羊抗大鼠 IgG 磁珠:Goat Anti-Rat IgG Magnetic Beads 20 mg

价格:¥

货号:S1433S
品牌:NEB

经销:基因有限公司

羊抗大鼠 IgG 磁珠,能少量分离纯化大鼠 IgG。羊抗大鼠 IgG 共价耦联到 1 μm 无孔顺磁颗粒上,此二抗能与大鼠的初级单克隆 IgG Fc 亚单位结合,适用于大鼠初级单克隆抗体 IgG 的免疫实验,还可以用大鼠 IgG 抗体定义细胞表面抗原来对细胞进行分离和分类。1 mg 磁珠能结合 5 μg IgG。

羊抗小鼠 IgG 磁珠:Goat Anti-Mouse IgG Magnetic Beads 20 mg

价格:¥

货号:S1431S
品牌:NEB

经销:基因有限公司

羊抗小鼠 IgG 磁珠,能少量分离纯化小鼠 IgG。羊抗小鼠 IgG 共价耦联到无孔顺磁颗粒上,此二抗能与小鼠IgG 各种亚型的重链相结合,适合用小鼠的初级单克隆抗体 IgG 的免疫实验,还可以用小鼠 IgG 抗体定义细胞表面抗原来对细胞进行分离和分类。 1 mg 磁珠能结合 5 μg IgG。

羊抗兔 IgG 磁珠:Goat Anti-Rabbit IgG Magnetic Beads 20 mg

价格:¥

货号:S1432S
品牌:NEB

经销:基因有限公司

羊抗兔 IgG 磁珠,能少量分离纯化兔 IgG。羊抗兔 IgG 共价耦联到无孔顺磁颗粒上,此二抗能与兔 IgG 各种亚型的重链相结合,适合用兔的初级多克隆抗体 IgG 的免疫实验,还可以用兔 IgG 抗体定义细胞表面抗原来对细胞进行分离和分类。 1 mg 磁珠能结合 5 μg IgG。

Protein A 磁珠:Protein A Magnetic Beads 1 ml

价格:¥

货号:S1425S
品牌:NEB

经销:基因有限公司

1 ml Protein A 或 Protein G 磁珠结合 > 400 μg 人 IgG。Protein A磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力,如:人、兔和小鼠。本品是将重组的截短型 Protein A 共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein A对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。 由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的,因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化,介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法,用所选一抗将目的蛋白从细胞粗提液中沉淀。此外,利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面,形成一个可重复使用的免疫共沉淀磁珠,避免了抗体与目的抗原共同洗脱。

Amylose 树脂 High Flow:Amylose Resin High Flow 15 ml

价格:¥

货号:E8022S
品牌:NEB

经销:基因有限公司

一种硬度增强了的微珠,更适合于全自动亲和层析系统,每毫升柱床体积可结合 6-8 mg MBP5-paramyosin ΔSal 融合蛋白。

Protein G 磁珠:Protein G Magnetic Beads 1 ml

价格:¥

货号:S1430S
品牌:NEB

经销:基因有限公司

Protein G 磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力,如:人、兔和小鼠。本品是将重组的截短型Protein G共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein G 对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。 由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的,因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化,介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法,用所选一抗将目的蛋白从细胞粗提液中沉淀。此外,利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面,形成一个可重复使用的免疫共沉淀磁珠,避免了抗体与目的抗原共同洗脱。

SNAP-捕获磁珠:SNAP-Capture Magnetic Beads 2 ml

价格:¥

货号:S9145S
品牌:NEB

经销:基因有限公司

耦联有苄基鸟嘌呤底物的磁性纤维素,可用于从溶液中选择性捕获或固定SNAP-tag 融合蛋白。这些微珠对 SNAP-tag融合蛋白结合容量很高,对复杂的细胞裂解液中的蛋白表现出极低的非特异性结合。这些特点使得该产品适于进行 pull-down 实验。

SNAP-捕获 Pull-Down 树脂:SNAP-Capture Pull-Down Resin 2 ml

价格:¥

货号:S9144S
品牌:NEB

经销:基因有限公司

耦联有苄基鸟嘌呤底物的琼脂糖珠,可用于从溶液中选择性捕获或固定SNAP-tag 融合蛋白。这些微珠对 SNAP-tag融合蛋白结合容量很高,对复杂的细胞裂解液中的蛋白表现出极低的非特异性结合。这些特点使得该产品适于进行 pull-down 实验。

亲水性链霉素亲和磁珠:Hydrophilic Streptavidin Magnetic Beads 5 ml

价格:¥

货号:S1421S
品牌:NEB

经销:基因有限公司

链霉素亲和磁珠由2μm超顺磁颗粒与高纯度链霉亲和素共价结合形成。磁珠可用于捕获生物素标记的底物,包括生物素标记的抗原、抗体和核酸。高特异性使得捕获的底物可满足后续实验要求,包括mRNA分离、捕获一抗、二抗抗体。这些磁珠通过独特的封闭试剂处理,因而磁珠表面特性更优,稳定性增强,非特异性结合显著降低,实验操作更方便,因此在涉及DNA免疫沉淀的应用中信噪比更佳。 2μm 无孔超顺磁微粒(#S1421)1 mg S1421可结合800 pmol以上的游离生物素,或400 pmol以上的生物素标记的20 bp单链寡核苷酸。磁珠以下列形式提供:4 mg/ml 磁珠悬浮在磷酸缓冲液(pH 7.4),0.1% BSA 和 0.02% NaN3组成的体系中。

几丁质珠:Chitin Resin 100 ml

价格:¥

货号:S6651L
品牌:NEB

经销:基因有限公司

一种亲和介质,能少量分离纯化与 CBD (Chitin Binding Domain)融合的目的蛋白。

几丁质磁珠:Chitin Magnetic Beads 25 ml

价格:¥

货号:E8036L
品牌:NEB

经销:基因有限公司

一种亲和介质,能少量分离纯化与 CBD (Chitin Binding Domain)融合的目的蛋白。几丁质磁珠是直径约为 50-70 μm 的顺磁微粒。100 μl 几丁质磁珠能结合 30-50 μg CBD 融合蛋白

Amylose 树脂:Amylose Resin 100 ml

价格:¥

货号:E8021L
品牌:NEB

经销:基因有限公司

每毫升柱床体积可结合 6-8 mg MBP5-paramyosin ΔSal 融合蛋白。

Amylose 树脂 High Flow:Amylose Resin High Flow 100 ml

价格:¥

货号:E8022L
品牌:NEB

经销:基因有限公司

一种硬度增强了的微珠,更适合于全自动亲和层析系统,每毫升柱床体积可结合 6-8 mg MBP5-paramyosin ΔSal 融合蛋白。

Protino 纯化柱 14ml:Protino Columns 14 mL 10 columns

价格:¥

货号:745250.10
品牌:MNG

经销:基因有限公司

empty columns (gravity flow )for use with, e.g., Protino® Ni-NTA Agarose

empty gravity-flow columns for use with, e.g., Protino resins, filter frits pack of 10

Protino 纯化柱 35ml:Protino Columns 35 mL 10 columns

价格:¥

货号:745255.10
品牌:MNG

经销:基因有限公司

empty columns (gravity flow)for use with, e.g., Protino® Ni-NTA Agarose

empty columns for gravity-flow use with, e.g., Protino resins, filter frits pack of 10

Protino Ni-IDA 1000 镍柱:Protino Ni-IDA 1000 packed columns 5 preps

价格:¥

货号:745160.5
品牌:MNG

经销:基因有限公司

Ni-IDA预装纯化柱,提供缓冲液。IDA螯合基团,3个蛋白结合位点提供更高结合载量
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-IDA 1000 Packed Columns, buffers
5 preps for the purification of His-tag proteins - Protino Ni-IDA 1000 Packed Columns, buffers

Protino Ni-TED 1000 镍柱:Protino Ni-TED 1000 packed columns 5 preps

价格:¥

货号:745110.5
品牌:MNG

经销:基因有限公司

Ni-TED预装纯化柱,提供缓冲液。Ted螯合基团,单个蛋白结合位点实现最高纯度和选择性
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-TED 1000 Packed Columns, buffers
5 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers

Protino Ni-IDA 2000 镍柱:Protino Ni-IDA 2000 packed columns 120g

价格:¥

货号:745170.5
品牌:MNG

经销:基因有限公司

Ni-IDA预装纯化柱,提供缓冲液。IDA螯合基团,3个蛋白结合位点提供更高结合载量
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-IDA 2000 Packed Columns, buffers
5 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers

Protino Ni-IVD 树脂:Protino Ni-IDA Resin 5g

价格:¥

货号:745210.5
品牌:MNG

经销:基因有限公司

Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins
• Chelating group IDA (iminodiacetic acid), three protein binding sites
• High protein yield / recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
• Dry resin → storage at room temperature

Protino® Ni-IDA Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-IDA Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin

Technology: IMAC, immobilized metal ion affinity chromatography
Chelating ligand: IDA (iminodiacetic acid)
Format: Bulk matrix
Matrix: Macroporous silica
Physical form: Dry matrix, precharged with Ni2+
Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume
Max. pressure 145 psi (10 bar)

* Binding capacity will vary for each polyhistidin-tagged protein

Applications
• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins
• Chelating group IDA (iminodiacetic acid), three protein binding sites
• High protein yield / recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
• Dry resin → storage at room temperature

Protino® Ni-IDA Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-IDA Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin

Technology: IMAC, immobilized metal ion affinity chromatography
Chelating ligand: IDA (iminodiacetic acid)
Format: Bulk matrix
Matrix: Macroporous silica
Physical form: Dry matrix, precharged with Ni2+
Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume
Max. pressure 145 psi (10 bar)

* Binding capacity will vary for each polyhistidin-tagged protein

Applications
• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications

Protino Ni-IDA Resin for the purification of His-tag proteins pack of 5 g

Protino Ni-TED 2000 镍柱:Protino Ni-TED 2000 packed columns 5 preps

价格:¥

货号:745120.5
品牌:MNG

经销:基因有限公司

Ni-TED预装纯化柱,提供缓冲液。Ted螯合基团,单个蛋白结合位点实现最高纯度和选择性
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-TED 2000 Packed Columns, buffers
5 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers

Protino Ni-TED 树脂:Protino Ni-TED Resin 5g

价格:¥

货号:745200.5
品牌:MNG

经销:基因有限公司

Ni-TED纯化填料螯合Ted基团,可耐受高压高流速,可用于FPLC,高亲和高选择性得到更纯的His-tag蛋白,在还原和螯合试剂中亦能保持高度稳定,极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供,灵活多用。
High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
• Dry resin → storage at room temperature

Protino® Ni-TED Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-TED Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin

Technology: IMAC (immobilized metal ion affinity chromatography)
Chelating ligand: TED (tris-carboxymethyl ethylene diamine)
Format: Bulk matrix
Matrix: Macroporous silica
Physical form: Dry matrix, precharged with Ni2+
Binding capacity* 10 mg/g resin, 5 mg/mL bed volume
Max. pressure 145 psi (10 bar)

* Binding capacity will vary for each polyhistidin-tagged protein

Applications
• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications

Protino Ni-TED Resin for the purification of His-tag proteins pack of 5 g

Protino Ni-IDA 150 镍柱:Protino Ni-IDA 150 packed columns 10 preps

价格:¥

货号:745150.10
品牌:MNG

经销:基因有限公司

Ni-IDA预装纯化柱,提供缓冲液。IDA螯合基团,3个蛋白结合位点提供更高结合载量
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-IDA 150 Packed Columns, buffers
10 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers

Protino Ni-TED 150 镍柱:Protino Ni-TED 150 packed columns 10 preps

价格:¥

货号:745100.10
品牌:MNG

经销:基因有限公司

Ni-TED预装纯化柱,提供缓冲液。Ted螯合基团,单个蛋白结合位点实现最高纯度和选择性
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
Protino® Ni-TED 150 Packed Columns, buffers
10 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers buffers

Protino Ni-NTA 镍柱:Protino Ni-NTA Column 5 mL 1 preps

价格:¥

货号:745415.1
品牌:MNG

经销:基因有限公司

预装FPLC柱。预装Ni-NTA Agarose,用于纯化His-tag蛋白。
Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose
Applications
• Purification of polyhistidine-tagged proteins
• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.)
FPLC™ columns
FPLC column (5 mL) for the purification of His-tag proteins pack of 1

Protino Ni-NTA 镍柱:Protino Ni-NTA Column 1 mL 5 preps

价格:¥

货号:745410.5
品牌:MNG

经销:基因有限公司

预装FPLC柱。预装Ni-NTA Agarose,用于纯化His-tag蛋白。
Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose
Applications
• Purification of polyhistidine-tagged proteins
• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.)
FPLC™ columns
FPLC columns (1 mL) for the purification of His-tag proteins pack of 5

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