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产品名/货号/品牌 简介 包装  
NEBNext High-Fidelity 2X PCR Master Mix, 50 rxns (50 μl vol) 50rxns

价格:¥

货号:M0541S
品牌:NEB

经销:基因有限公司

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化,不论GC含量多少,该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂,适合大多数模板的扩增,包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

NEBNext® Q5® Hot Start HiFi PCR Master Mix, 50 reactions 50 reactions

价格:¥

货号:M0543S
品牌:NEB

经销:基因有限公司

为新一代测序文库扩增优化的高性能超保真扩增酶预混液,超过常规Taq酶100倍的超高保真度,超强扩增能力,可用于高GC含量模板,是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix
The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

NEBNext High-Fidelity 2X PCR Master Mix, 250 rxns (50 μl vol) 250rxns

价格:¥

货号:M0541L
品牌:NEB

经销:基因有限公司

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化,不论GC含量多少,该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂,适合大多数模板的扩增,包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

NEBNext® Q5® Hot Start HiFi PCR Master Mix, 250 reactions 250 reactions

价格:¥

货号:M0543L
品牌:NEB

经销:基因有限公司

为新一代测序文库扩增优化的高性能超保真扩增酶预混液,超过常规Taq酶100倍的超高保真度,超强扩增能力,可用于高GC含量模板,是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix
The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

VAHTSTM Super-Fidelity DNA Polymerase 24 rxn

价格:¥

货号:N206-01
品牌:Vazyme

经销:南京诺唯赞生物

VAHTSTM Super-Fidelity DNA Polymerase是针对高通量测序平台文库构建定向优化而成的高保真聚合酶。该酶是一种基于Pfu DNA Polymerase改造而成的新一代高保真DNA聚合酶,具有极高的扩增效率和广泛的模板适应性。和Pfu DNA Polymerase相比,VAHTSTM Super-Fidelity DNA Polymerase的行进性得到的大幅度提升,即使是非常复杂的模板也能快速准确的完成PCR反应。其错配率是Taq DNA Polymerase的1/52,是P

VAHTSTM Super-Fidelity DNA Polymerase 96 rxn

价格:¥

货号:N206-02
品牌:Vazyme

经销:南京诺唯赞生物

VAHTSTM Super-Fidelity DNA Polymerase是针对高通量测序平台文库构建定向优化而成的高保真聚合酶。该酶是一种基于Pfu DNA Polymerase改造而成的新一代高保真DNA聚合酶,具有极高的扩增效率和广泛的模板适应性。和Pfu DNA Polymerase相比,VAHTSTM Super-Fidelity DNA Polymerase的行进性得到的大幅度提升,即使是非常复杂的模板也能快速准确的完成PCR反应。其错配率是Taq DNA Polymerase的1/52,是P

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