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分类简介:Tyramide Signal Amplification (TSA™) from PerkinElmer is a technology that improves fluorescent and chromogenic signals by up to 100-fold while allowing reduced consumption of primary antibodies or probes. 

TSA Plus is an optimized and enhanced version that is up to 20 times more sensitive than standard tyramide reagents. TSA Plus Fluorescence Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of fluorophores directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can then be viewed directly using standard or confocal microscopy. TSA Plus Fluorescein may also be used in combination with anti-fluorescein enzyme conjugates and appropriate chromogenic substrates for brightfield microscopy.

The use of TSA reagents results in a significant increase in sensitivity over standard detection methods, while maintaining specificity and resolution. Moreover, TSA reagents allow drastically reduced consumption of primary antibody or probe. TSA Plus Fluorescence kits are ideal for multiplexed IHC or ISH in combination. 

Detect & quantify low abundance analytes
Improve performance of weakly binding primary antibodies
Use less antibody & probe for improved background with reduced cost 
Reduce scanning time producing images faster


TSA Selection Guide   

TSA Plus Kits for direct fluorescence detection(TSA Plus Kits provides ~20X improved sensitivity over standard tyramide reagents)
TSA Plus Fluorescein   494 nm 517 nm
TSA Plus TMR            550 nm 570 nm
TSA Plus Cyanine 3     550 nm 570 nm
TSA Plus Cyanine 3.5  581 nm 596 nm
TSA Plus Cyanine 5     648 nm 667 nm
TSA Plus Cyanine 5.5  673 nm 692 nm

TSA Plus Kits for chromogenic or indirect fluorescence detection(TSA Plus Kits provides ~20X improved sensitivity over standard tyramide reagents) 
TSA Plus Biotin         Biotin
TSA Plus DIG           Digoxigenin
TSA Plus Fluorescein Fluorescein
TSA Plus DNP          2,4 Dinitrophenyl

TSA Kits, original formulation 
TSA Systems for direct fluorescence detection
TSA Coumarin    402 nm 443 nm
TSA Fluorescein  494 nm 517 nm
TSA TMR           550 nm 570 nm
TSA Cyanine 3    550 nm 570 nm
TSA Cyanine 5    648 nm 667 nm

TSA Systems for chromogenic or indirect fluorescence detection
TSA Biotin
TSA Fluorescein

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