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生物通商城首页 -> 试剂 > 表观遗传学 > AlphaLISA表观遗传学工具

表观遗传学

DNA修饰的检测定量

甲基化敏感酶/相关酶

组蛋白/DNA甲基转移酶

组蛋白分析

重亚硫酸盐处理的PCR

染色质免疫共沉淀

甲基化DNA免疫沉淀

表观遗传学相关抗体

AlphaLISA表观遗传学工具

DNA对照和辅助

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分类简介:AlphaLISA technology is a no-wash homogeneous proximity immunoassay that includes Alpha Donor and AlphaLISA Acceptor beads to quantitate activity on histone, peptide, or protein substrates. In vitro methylation or acetylation of substrate is performed by incubating enzyme (for example, a histone acetyltransferase or methyltransferase) with S-adenosylmethionine (SAM) or Acetyl CoA cofactor and histone substrate. Following the enzymatic reaction, methylation or acetylation is monitored using mark-specific anti-methyl or anti-acetyl antibody-conjugated AlphaLISA Acceptor beads. Once streptavidin-coated Donor beads are added to capture the substrate, the beads are brought into close proximity. The Donor beads are excited by a laser at 680 nm to release singlet oxygen. The singlet oxygen causes a reaction in the Acceptor bead and light emission is produced at 615 nm. This emission is proportional to the amount of methylated or acetylated substrate present. 
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产品名/货号/品牌 简介 包装  
Alpha乙酰化的组蛋白H3(H3K27ac)定量检测试剂盒:AlphaLISA H3K27ac cellular detection kit 100次(数据点)

价格:¥

货号:AL720HV
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K27me2-1)定量检测试剂盒:AlphaLISA H3K27me2-1 cellular detection kit 100次(数据点)

价格:¥

货号:AL721HV
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K36me2)定量检测试剂盒:AlphaLISA H3K36me2 cellular detection kit 100次(数据点)

价格:¥

货号:AL723HV
品牌:PERKINELMER

经销:基因有限公司

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput. Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6 The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Alpha乙酰化的组蛋白H3(H3K9ac)定量检测试剂盒:AlphaLISA H3K9ac cellular detection kit 100次(数据点)

价格:¥

货号:AL714HV
品牌:PERKINELMER

经销:基因有限公司

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput. Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6 The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

生物素标记的抗组蛋白H3 C末端抗体 :Biotinylated anti-Histone H3 (C-terminal) ant 2 µg

价格:¥

货号:AL118C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27ac受体微珠:AlphaLISA anti-H3K27ac Acceptor beads 0.25 mg

价格:¥

货号:AL120C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27me2-1受体微珠:AlphaLISA anti-H3K27me2-1 Acceptor beads 0.25 mg

价格:¥

货号:AL121C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27me3受体微珠:AlphaLISA anti-H3K27me3 Acceptor beads 0.25 mg

价格:¥

货号:AL122C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K4 (unmodified)受体微珠:AlphaLISA anti-H3K4 (unmodified) Acceptor bea 0.25 mg

价格:¥

货号:AL119C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K4me1-2受体微珠:AlphaLISA anti-H3K4me1-2 Acceptor beads 0.25 mg

价格:¥

货号:AL116C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K9/ K27 unmodified受体微珠:AlphaLISA anti-H3K9/ K27 unmodified Acceptor 0.25 mg

价格:¥

货号:AL138C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K9me2受体微珠:AlphaLISA anti-H3K9me2 Acceptor beads 0.25 mg

价格:¥

货号:AL117C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3R2me2受体微珠:AlphaLISA anti-H3R2me2 Acceptor beads 0.25 mg

价格:¥

货号:AL139C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-p53K382ac受体微珠:AlphaLISA anti-p53K382ac Acceptor beads 0.25 mg

价格:¥

货号:AL124C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K9ac受体微珠:AlphaLISA anti-H3K9ac Acceptor beads 0.25 mg

价格:¥

货号:AL114C
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K36me2受体微珠:AlphaLISA anti-H3K36me2 Acceptor beads 0.25 mg

价格:¥

货号:AL123C
品牌:PerkinElmer

经销:基因有限公司

Alpha乙酰化的组蛋白H3(H3K27ac)定量检测试剂盒:AlphaLISA H3K27ac cellular detection kit 500次(数据点)

价格:¥

货号:AL720C
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K27me2-1)定量检测试剂盒:AlphaLISA H3K27me2-1 cellular detection kit 500次(数据点)

价格:¥

货号:AL721C
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K27me3)定量检测试剂盒:AlphaLISA H3K27me3 cellular detection kit 500次(数据点)

价格:¥

货号:AL722C
品牌:PerkinElmer

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K36me2)定量检测试剂盒:AlphaLISA H3K36me2 cellular detection kit 500次(数据点)

价格:¥

货号:AL723C
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K4me2)定量检测试剂盒:AlphaLISA H3K4me2 cellular detection kit 500次(数据点)

价格:¥

货号:AL716C
品牌:PerkinElmer

经销:基因有限公司

Alpha乙酰化的组蛋白H3(H3K9ac)定量检测试剂盒:AlphaLISA H3K9ac cellular detection kit 500次(数据点)

价格:¥

货号:AL714C
品牌:PERKINELMER

经销:基因有限公司

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput. Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6 The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

生物素标记的抗组蛋白H3 C末端抗体 :Biotinylated anti-Histone H3 (C-terminal) ant 5 mg

价格:¥

货号:AL118M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3 C-terminus受体微珠:AlphaLISA anti-H3 C-terminus Acceptor beads 5 mg

价格:¥

货号:AL147M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27ac受体微珠:AlphaLISA anti-H3K27ac Acceptor beads 5 mg

价格:¥

货号:AL120M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27me2-1受体微珠:AlphaLISA anti-H3K27me2-1 Acceptor beads 5 mg

价格:¥

货号:AL121M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K27me3受体微珠:AlphaLISA anti-H3K27me3 Acceptor beads 5 mg

价格:¥

货号:AL122M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K36me2受体微珠:AlphaLISA anti-H3K36me2 Acceptor beads 5 mg

价格:¥

货号:AL123M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K4 (unmodified)受体微珠:AlphaLISA anti-H3K4 (unmodified) Acceptor bea 5 mg

价格:¥

货号:AL119M
品牌:PerkinElmer

经销:基因有限公司

AlphaLISA anti-H3K4me1-2受体微珠:AlphaLISA anti-H3K4me1-2 Acceptor beads 5 mg

价格:¥

货号:AL116M
品牌:PerkinElmer

经销:基因有限公司

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