Cas9 Nuclease, S. pyogenes, 50 pmol cas9核酸酶，是一种能够催化双链DNA特异位点切割的导向RNA内切酶，50pmol，1000nM相当于159ng/ul。

Cas9 核酸酶，S. pyogenes  Cas9 Nuclease, S. pyogenes, 300 pmol，Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

是 RNA 介导的核酸酶，可以催化双链 DNA 的特异位点切割。其在PAM（Protospacer Adjacent Motif）上 NGG 处，靶序列的 3 个碱基内进行切割。EnGen Cas9 NLS, S. pyogenes, is an RNA-guided endonuclease nuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Cas9 NLS, S. pyogenes contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.

是 RNA 介导的核酸酶，可以催化双链 DNA 的特异位点切割。其在PAM（Protospacer Adjacent Motif）上 NGG 处，靶序列的 3 个碱基内进行切割。EnGen Cas9 NLS, S. pyogenes, is an RNA-guided endonuclease nuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Cas9 NLS, S. pyogenes contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.

Cas9 Nuclease是一个RNA导向的序列特异性双链DNA内切酶。向导RNA通过与靶序列互补来识别靶位点，并将Cas9 Nuclease带到靶 DNA上。Cas9 Nuclease有两个切割活性中心，分别切割靶DNA的两条链从而产生DNA双链断裂。切割位点为靶向区域内和NGGPAM相距三个碱基处。本产品是克隆Streptococcus pyogenes的Cas9基因后在大肠杆菌中表达纯化的高纯度Cas9活性蛋白。

Cas9 Nuclease是一个RNA导向的序列特异性双链DNA内切酶。向导RNA通过与靶序列互补来识别靶位点，并将Cas9 Nuclease带到靶 DNA上。Cas9 Nuclease有两个切割活性中心，分别切割靶DNA的两条链从而产生DNA双链断裂。切割位点为靶向区域内和NGGPAM相距三个碱基处。本产品是克隆Streptococcus pyogenes的Cas9基因后在大肠杆菌中表达纯化的高纯度Cas9活性蛋白。