20 µg转染即用型，纯化的质粒DNA；更多可达20次转染；Axl Double Nickase Plasmid (h) 含有一对质粒，分别编码D10A 突变的Cas9核酸酶，和目标特异的 20 nt 向导RNA µgRNA），与其相应的CRISPR/Cas9敲除质粒相比，它在基因敲除方面具有更高的特异性；成对的向导RNA序列被将近20个碱基对抵消了，考虑到基因组DNA特异的Cas9调停双切口，类似于DSB位点；一个质粒对包含了一个供筛选使用的嘌呤霉素抗性基因；其他质粒对包含了供观察转染使用的绿色荧光蛋白标记；转染结束后，基因敲除效率可用WB, IF或者IHC验证:?Axl (B-2): sc-166268或通过WB, IF, 或IHC用抗体:Axl (C-20): sc-1096；20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections；Axl Double Nickase Plasmid (h) consists of a pair of plasmids each encodi µg a D10A mutated Cas9 nuclease and a ta µget-specific 20 nt µguide RNA µgRNA) des µgned to knockout µgene expression with µgreater specificity than its CRISPR/Cas9 KO counterpart；Paired µgRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicki µg of the µgenomic DNA, which mimics a DSB；One plasmid in the pair contains a puromycin-resistance µgene for selection; the other plasmid in the pair contains a µgFP marker to visually confirm transfection.
20 µg纯化即用型质粒DNA，更多可供20次转染Cre Vector表达Cre重组酶，催化两个LoxP位点之间的位点特异性DNA重组Cre Vector is designed to specifically target the LoxP sites flanking selection markers inserted by the HDR Plasmid and excise the genetic materialCre Vector含有一个CMV启动子，以启动Cre重组酶的表达转染后，可以用针对目的基因的抗体，通过WB、IF或IHC分析细胞；或继续转染，做其他DNA编辑
20 µg of transfection-ready, purified plasma DNA; Suitable for up to 20 transfectionsControl Double Nickase Plasmid is provided as a negative control for Double Nickase PlasmidsControl Double Nickase Plasmid consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a unique, non-targeting 20 nt scramble guide RNA (gRNA)One plasmid in the pair contains a puromycin-resistance gene; the other plasmid in the pair contains a GFP marker to visually confirm transfection
200 µl of transduction-ready, high-titer Control Lentiviral Activation Particles, sufficient for 10-20 transductionsControl Lentiviral Activation Particles are provided as a negative control for CRISPR/dCas9 Lentiviral Activation ParticlesControl Lentiviral Activation Particles contain the following SAM activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein, and a non-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genesThe SAM complex does not recognize or bind any specific sequence within the genomic DNA, and does not activate transcription of any specific geneAliquot and store at -80º C
20 µg of transfection-ready, purified plasma DNA; Suitable for up to 20 transfectionsControl CRISPR Activation Plasmid is provided as a negative control for CRISPR Activation PlasmidsControl CRISPR Activation Plasmid consists of the following three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64; a plasmid encoding the MS2-p65-HSF1 fusion protein; and a plasmid encoding a non-specfific 20 nt guide RNA (gRNA)
20 µg of transfection-ready, purified plasma DNA; Suitable for up to 20 transfectionsControl CRISPR/Cas9 Plasmid is a plasmid encoding the Cas9 nuclease and a non-specific 20 nt guide RNA (gRNA) to be used as a negative control for CRISPR/Cas9 Knockout Plasmids
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