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          生物通商城首页 > 试剂 > 表观遗传学 > 染色质免疫共沉淀

          SimpleChIP® Enzymatic Chromatin IP Kit (Magne

          品牌:CST 货号:9003S 立即咨询 QQ 咨询留言

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          ¥4875.00目录价:¥6500.00

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          ¥4875.00
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          • 基因有限公司 发货并提供售后服务

          Shelf Life(month):24
          产地:USA
          存储条件:2-8 & -20
          Reactivity: H, M, R, Mk.1 Kit(30 immunoprecipitations). The SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) contains the buffers and reagents necessary to perform up to 6 chromatin preparations and 30 chromatin immunoprecipitations and is optimized for 4 X 107 cells per experiment. A complete assay can be performed in as little as two days and can easily be scaled up or down for use with more or fewer cells. 
 Cells are fixed with formaldehyde and lysed, and chromatin is fragmented by partial digestion with Micrococcal Nuclease to obtain chromatin fragments of 1 to 5 nucleosomes. Enzymatic fragmentation of chromatin is much milder than sonication and eliminates problems resulting from variability in sonication power and emulsification of chromatin during sonication, which can result in incomplete fragmentation of chromatin or loss of antibody epitopes due to protein denaturation and degradation. Chromatin immunoprecipitations are performed using ChIP-validated antibodies and ChIP-Grade Protein G Agarose Beads. After reversal of protein-DNA cross-links, the DNA is purified using DNA purification spin columns, allowing for easy and efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. The enrichment of particular DNA sequences during immunoprecipitation can be analyzed by standard PCR, quantitative real-time PCR, or amplification for ChIP on chip, sequencing or cloning techniques. 
 The SimpleChIP® Kit also provides important controls to ensure a successful ChIP experiment. The kit contains a positive control Histone H3 Antibody, a negative control Normal Rabbit IgG Antibody and primer sets for PCR detection of the human and mouse ribosomal protein L30 (RPL30) genes. Histone H3 is a core component of chromatin and is bound to most DNA sequences throughout the genome, including the RPL30 locus. Thus, the Histone H3 Antibody provides a universal positive control that should enrich for almost any locus examined.

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