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          生物通商城首页 > 试剂 > 免疫学 > Western Blot免疫印迹 > WB发光检测

          Phototope-HRP Western Blot Detection System,

          品牌:CST 货号:7072S 立即咨询 QQ 咨询留言

          生物通价:

          ¥2609.00目录价:¥3478.00

          生物通价:

          ¥2609.00
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          • 基因有限公司 发货并提供售后服务

          Shelf Life(month):24
          产地:USA
          存储条件:-20
          50次反应。试剂盒含发光底物,HRP偶联的抗小鼠二抗和生物素标记蛋白分子量标记,灵敏度高,发光持续时间长,可检测亚皮克级的蛋白质。为标准的western blotting 应用中检测化学发光的蛋白而设计。蛋白和生物素化的分子量标记(已提供)可以在SDS-PAGE中分离,再被转移到膜上。与抗血清的一抗和辣根过氧化物酶(HRP) 标记的二抗孵育后,HRP标记的抗生物素抗体被结合,然后与LumiGLO ® 试剂反应,发出的光随后被捕获到X胶片上。
          Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays.
          20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
          Anti-mouse IgG, HRP-linked Antibody #7076 250 µl Goat
          Biotinylated Protein Ladder Detection Pack #7727 500 µl
          LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin
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          • 咨询标题:[咨询产品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
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