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          生物通商城首页 > 试剂 > 细胞生物学 > 增殖/活力/毒性分析 > 细胞增殖分析

          BrdU 细胞增殖分析试剂盒

          BrdU Cell Proliferation Assay Kit

          品牌:CST 货号:6813S 立即咨询 QQ 咨询留言

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          ¥5270.00目录价:¥7026.00

          生物通价:

          ¥5270.00
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          Shelf Life(month):24
          产地:USA
          存储条件:-20
          BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU,使用的抗体为抗BrDU抗体。当细胞培养于含BrDU的标记培养基中,该胸腺嘧啶类似物可代替胸腺嘧啶掺入到增殖细胞的新合成DNA中。吸除标记培养基后,用固定/变性液固定细胞同时使DNA变性。然后加入BrdU鼠源单抗检测掺入的BrdU(DNA变性使得掺入的BrdU易于为抗体所识别)。Anti-mouse IgG, HRP-linked antibody用来识别联胞检测抗体。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和掺入细胞的BrdU的量成正比, BrdU是细胞增殖的直接证明。BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU。BrDU标记的DNA变性后能够为试剂盒中的BrdU Mouse mAb检测到。BrdU Mouse mAb不与内源性DNA发生交叉反应。依细胞种类和孵育时间不同 ,每孔0.2-2x104个细胞即足够大部分实验。为了得到最佳实验结果,我们推荐进行细胞数滴度实验
          500 assays (96 well format). The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Then a BrdU mouse mAb is added to detect the incorporated BrdU (The denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
          BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups.
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