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          生物通商城首页 > 试剂 > 核酸纯化 > DNA片段纯化 > 凝胶DNA回收/离心柱

          NucleoSpin 胶/PCR产物纯化试剂盒

          NucleoSpin Gel and PCR Clean-up

          品牌:MNG 货号:740609.50 立即咨询 QQ 咨询留言

          生物通价:

          ¥606.00目录价:¥993.60

          生物通价:

          ¥606.00
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          库存货期:
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          • 基因有限公司 发货并提供售后服务

          Shelf Life(month):48
          产地:GERMANY
          存储条件:常温
          胶回收与PCR 产物回收纯化二合为一,高效回收大于50bp的片段。回收率高,洗脱体积小(15uL),从而可获得高浓度的DNA回收产物。适用于TAE,TBE等各种胶缓冲系统。10分钟完成,省时省力。适用于PCR 产物纯化;从凝胶中抽提DNA 样本;浓缩反应产物以及去除盐离子;可以纯化浓缩单链DNA (需要NTC 缓冲液);可以去除样本中SDS 杂质(需要NTB 缓冲液)。纯化后的产物适用于:克隆、测序、PCR、限制酶内切等实验
          硅胶膜技术 微型离心柱
          样本 <400 μL PCR 反应产物 < 400 mg TAE/TBE agarose胶
          回收片段大小 50bp – 15 kbp
          回收效率 70 – 95 %
          A260/A280: 1.8 – 1.9
          洗脱体积: 15 – 50 μL
          制备时间 10 min / 6 preps
          载量 25μ g
          PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume
          • Two applications in one kit, one buffer with optimal performance for both applications
          • High recoveries for fragments down to 50 bp
          • Minimized elution volume: 15 μL – highly concentrated DNA
          • Binding buffer with pH indicator
          • Separate buffers for single stranded DNA or SDS containing samples available
          • Suitable for all gel buffer systems (e.g., TAE, TBE)
          • Also available for QIAcube® (please see "Ordering information" and contact Technical Service
          at techbio@mn-net.com for more information).

          Technology: Silica-membrane technology
          Format: Mini spin columns
          Sample material: < 400 µL PCR reaction mixture 、 < 400 mg TAE / TBE agarose gel
          Fragment size: 50 bp–approx. 20 kbp
          Typical recovery: 70–95%
          A260/A280: 1.8–1.9
          Elution volume 15–30 µL
          Preparation time 10 min/6 preps
          Binding capacity 25 µg

          Applications
          • Purification of PCR products
          • Extraction of DNA from agarose gels
          • NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
          - nucleotides, primers
          - enzymes
          - mineral oil
          - PCR additives (e.g., salts, betaine, DMSO)
          - detergents (e.g., Tween 20, Triton X-100)
          - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
          • Support protocol for DNA extraction from polyacrylamide gels
          • Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from
          agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
          • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
          kit, see “Ordering information”)

          • Typical downstream applications: cloning, sequencing, PCR, restriction analysis
          NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers
          50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

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          • 咨询标题:[咨询产品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
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