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核酸纯化

质粒提取纯化

基因组DNA分离

RNA提取纯化

病毒核酸纯化

总RNA/DNA/蛋白同步分离

DNA片段纯化

样品采集与保存系统

高通量耗材

核酸助沉淀剂

缓冲液和辅助产品

FFPE样品处理

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凝胶DNA回收/离心柱 >> NucleoSpin Gel and PCR Clean-up (50)

品牌:MNG 货号:740609.50

目录价:¥966.00

服务:基因有限公司 发货并提供售后服务

包装:50

一般货期:5天 (货期仅供参考,具体请来电或在线确认)

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Shelf Life(month):48
产地:GERMANY
存储条件:室温
胶回收与PCR 产物回收纯化二合为一,高效回收大于50bp的片段。回收率高,洗脱体积小(15uL),从而可获得高浓度的DNA回收产物。适用于TAE,TBE等各种胶缓冲系统。10分钟完成,省时省力。适用于PCR 产物纯化;从凝胶中抽提DNA 样本;浓缩反应产物以及去除盐离子;可以纯化浓缩单链DNA (需要NTC 缓冲液);可以去除样本中SDS 杂质(需要NTB 缓冲液)。纯化后的产物适用于:克隆、测序、PCR、限制酶内切等实验
硅胶膜技术 微型离心柱
样本 <400 μL PCR 反应产物 < 400 mg TAE/TBE agarose胶
回收片段大小 50bp – 15 kbp
回收效率 70 – 95 %
A260/A280: 1.8 – 1.9
洗脱体积: 15 – 50 μL
制备时间 10 min / 6 preps
载量 25μ g
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume
• Two applications in one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
• Also available for QIAcube® (please see "Ordering information" and contact Technical Service
at techbio@mn-net.com for more information).

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 400 µL PCR reaction mixture 、 < 400 mg TAE / TBE agarose gel
Fragment size: 50 bp–approx. 20 kbp
Typical recovery: 70–95%
A260/A280: 1.8–1.9
Elution volume 15–30 µL
Preparation time 10 min/6 preps
Binding capacity 25 µg

Applications
• Purification of PCR products
• Extraction of DNA from agarose gels
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
• Support protocol for DNA extraction from polyacrylamide gels
• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from
agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
kit, see “Ordering information”)

• Typical downstream applications: cloning, sequencing, PCR, restriction analysis
NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers
50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

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