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生物通商城首页 > 试剂 > 核酸纯化 > RNA提取纯化 > 小分子RNA提取

NucleoSpin miRNA 提取试剂盒

NucleoSpin miRNA

品牌:MNG 货号:740971.50 咨询留言

生物通价:

¥1925.00目录价:¥3155.60

生物通价:

¥1925.00
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  • 基因有限公司 发货并提供售后服务

Shelf Life(month):12
产地:GERMANY
存储条件:常温
方便快速地提取< 200 b的小分子RNA,离心柱式多用途试剂盒也可以分别提取小分子RNA和大分子RNA,以及蛋白质组分。出色的小分子RNA回收率,无需酚氯仿,提供NucleoSpin® Filters有效降低裂解液粘稠度便于均质化,提供rDNase去除DNA污染
Parallel isolation of small and large RNA
• RNA purification fractionated by size: Isolation of small RNA only (< 200 b),
Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
• NucleoSpin® Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 10(7) cultured cells, < 30 mg human / animal tissue; < 50 mg plant tissue, < 150 µL reaction mixture
Fragment size: Small RNA: < 200 b, large RNA: > 200 b
Typical yield: 10 µg small RNA, 95 µg large RNA from 10(7) HeLa cells
Elution volume: 30–100 µL
Preparation time: < 45 min (6 preps human / animal tissue, small and large RNA)
< 35 min (6 preps human / animal tissue, small RNA only)
Binding capacity 200 µg

Applications**
• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol®) lysis
• Purification of siRNA and large dsRNA from DICER reactionsTypical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization. Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
NucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of small and large RNA - NucleoSpin RNA Columns, NucleoSpin miRNA Columns, NucleoSpin Filters, NucleoSpin Protein Removal Columns, Collection Tubes, buffers, RNase-free rDNase

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  • 咨询标题:[咨询产品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
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