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          生物通商城首页 > 试剂 > 分子生物学 > RNA分析 > 逆转录/cDNA合成

          SensiFAST cDNA快速合成试剂盒

          SensiFAST cDNA Synthesis Kit

          品牌:Bioline 货号:BIO-65053 立即咨询 QQ 咨询留言

          1、灵敏性高,模板起始量低至1pg总RNA,适用于低拷贝模板扩增 2、无偏cDNA合成,均一扩增全长的RNA 3、快速逆转录,反应30分钟以内完成 4、新型逆转录酶和buffer系统,保证产品性能的可重现性

          暂无报价:

          +特殊运费:
          • ¥644.00 (需额外收取)
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          • 基因有限公司 发货并提供售后服务

          模板类型:RNA
          产地:Germany
          存储条件:-20
          cDNA快速合成试剂盒(50次),将RNA逆转录成cDNA,试剂盒中含逆转录酶、TransAmp buffer等反应所需组分,配制反应体系更方便快捷,减少污染风险。起始模板量可以低至1pg总RNA,适用于低拷贝模板扩增,RNA全长均一扩增性能卓越 第一链cDNA合成试剂盒,灵敏度高,可从少至1pg启始总RNA中制备第一链cDNA。Developed to generate high-quality first-strand cDNA from total RNA template. The kit contains all necessary components in a unique TransAmp™ buffer system to deliver the highest quality cDNA.

          Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA
          Broad dynamic range – ideal for dilute and low-copy samples
          Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA
          Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation
          Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications
          Applications
          Two-step RT-PCR
          Two-step RT-qPCR

          Description
          SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

          A reverse transcriptase and new TransAmp™ buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.
          1、灵敏性高,模板起始量低至 1 pg 总 RNA,适用于低拷贝模板扩增。 2、无偏 cDNA 合成,均一扩增全长的 RNA。 3、快速逆转录,反应 30 分钟以内完成。 4、新型逆转录酶和 buffer 系统,保证产品性能的可重现性。
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          • 咨询标题:[咨询产品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
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