微信下单
全部分类

最新加入的商品

          • 0件商品
            共计:¥0.00
          共计¥0.00
          去购物车
          • 促销专题
          生物通商城首页 > 试剂 > 免疫学 > 免疫沉淀 > MeDIP/甲基化DNA免疫沉淀

          SimpleDIP™ Hydroxymethylated DNA IP (hMeDIP)

          品牌:CST 货号:95176S 立即咨询 QQ 咨询留言

          生物通价:

          ¥3617.00目录价:¥4822.00

          生物通价:

          ¥3617.00
          选择包装:
          库存货期:
          服  务:
          • 基因有限公司 发货并提供售后服务

          产地:USA
          存储条件:2-8 & -20
          SimpleDIP™羟甲基化DNA免疫沉淀试剂盒;应用MeDIP ;储存温度4℃ and -20℃;
          The SimpleDIP™ Hydroxymethylated DNA IP (hMeDIP) Kit provides enough reagents to perform up to 10 genomic DNA preparations and 10 IPs from mammalian cells and is optimized for 1 μg of genomic DNA per IP. The SimpleDIP™ protocol can be performed in as little as two days and can easily be scaled up or down for use with more or less cells. Cells are first lysed and genomic DNA is extracted and sonicated into small fragments (200-500 bp). DNA IPs are performed using 5-Hydroxymethylcytosine (5-hmC) (HMC31) Mouse mAb and ChIP-Grade Protein G Magnetic Beads. After elution from the beads, the DNA is purified using DNA purification spin columns provided in the kit. The enrichment of particular DNA sequences can be analyzed by a variety of methods including standard PCR, quantitative real-time PCR, or next-generation sequencing. The SimpleDIP™ 5-Hydroxymethylcytosine DNA IP Kit provides a highly validated 5-hmC monoclonal antibody to ensure specific and robust signal. The kit also includes DNA that contains exclusively 5-hydroxymethylcytosine, which can be spiked-in to the IPs as a control. Thus, spiked-in DNA will be immunoprecipitated with 5-Hydroxymethylcytosine (HMC1) Mouse mAb, but not with the Mouse (G3A1) mAb IgG1 Isotype Control (DIP Formulated). The relative enrichment can then be quantified using the SimpleDIP Hydroxymethyl Control Primers.
          • 产品咨询
          • 咨询标题:[咨询产品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
          • 咨询内容:
          • 联系方式: (可以是手机,座机[带区号],Email,QQ等)
          • 验证码:
          • 常见问题(0条)
          常见问题
          • 产品评价( 0 条)
          产品评价
          好评度 98%
          • 不错
          • 不错123
          • 不错234543
          • 不错2020
          • 不错2020
          • 不错2020
          • 不错
          全部评价(1)
           只看当前商品评价
          张三

          黄金会员

          不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错不错

          生物通 版权所有

          未经书面授权,所有页面内容、图片和设计不得以任何形式进行复制

          Copyright © 2000-2018 eBiotrade.com, All Rights Reserved

          本栏目客服联系电话:广州 020-85524840转1060

          本栏目广告联系电话:广州 020-87511980

          主编信箱:

          粤公网安备 44010602000434号 粤ICP备09063491号