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          生物通商城首页 > 试剂 > 细胞生物学 > 活体成像试剂 > 生物发光荧光素底物

          XenoLight D-Luciferin生物发光底物

          XenoLight D-Luciferin - K+ Salt Bioluminescen

          品牌:PERKINELMER 货号:122799 立即咨询 QQ 咨询留言

          荧光素能够通过血脑屏障,血胎盘屏障和血睾丸屏障,毒性低。

          生物通价:

          ¥7840.00目录价:¥9800.00

          生物通价:

          ¥7840.00
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          产地:USA
          存储条件:常温
          发光类型:近红外荧光
          荧光素是一种低分子量有机化合物,由苯并噻唑基和噻唑羧酸基组成。荧光素是在萤火虫和和其他有荧光素酶动物中被发现的,在ATP和酶荧光素的催化下发光。荧光素分子较小,免疫原性低/抗原性低。荧光素能够通过血脑屏障,血胎盘屏障和血睾丸屏障,毒性低。D-Luciferin是生物体的细胞中发现的一种化学物质,在荧光素酶和ATP的催化作用下产生光。荧光素能够穿透细胞膜,可以用于监控体内转导及表达荧光素酶的细胞活动,因为荧光素酶的反应是ATP依赖,可检测细胞活性。
          Luciferin is a low molecular weight organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is found in fireflies and other animals which, in the presence of ATP and the enzyme luciferase, becomes luminescent. The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.How do you administer luciferin? Mice with lux-bearing bacteria do not need luciferin to glow. In the tumor models and transgenic models, luciferin is administered intraperitoneally (concomitant with anesthesia). How well does luciferin distribute? Luciferin distributes quickly and easily throughout the animal. How do the animals respond to the repeated administration of luciferin substrate? Luciferin does not affect the animals deleteriously (no evidence of toxicological or immunological effects). Do you need to administer luciferin substrate to the animals before imaging? In bacteria, the entire luciferase operon is stably integrated on the chromosome. This eliminates the need for exogenous luciferin substrate in the bacterial models. The tumor models and transgenic models rely on the exogenous administration of luciferin.
          http://www.perkinelmer.com/lab-solutions/resources/docs/SOP_LuciferinPrep_InVitroInVivo_BLI-Assays.pdf
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