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          生物通商城首页 > 试剂 > 分子生物学 > 培养基

          NEBNext DirectTM Cancer HotSpot Panel

          NEBNext Direct Cancer HotSpot Panel, 8 rxns

          品牌:NEB 货号:E7000S 立即咨询 QQ 咨询留言

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          ¥14279.00目录价:¥16799.00

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          ¥14279.00
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          • 基因有限公司 发货并提供售后服务

          产地:USA
          存储条件:2-8 & -20
          靶向富集,配合 NGS 技术,使对基因组中目标区域高通量、高深度的测序成为可能。 NEBNext Direct 是一种全新的,基于杂交的捕获方法,与传统的液相杂交及多重 PCR 方法相比有明显的优势。 在 NEBNext Direct 靶向富集方法中(图1),片段化的 DNA 迅速杂交到生物素化的寡聚核苷酸饵上,该寡聚核苷酸定义了目标序列 3’ 端序列。核苷酸饵-目标序列杂交体结合到链霉亲和素磁珠上,而任何 3’ 脱靶序列则通过酶学法去除。短杂交时间搭配酶学法去除 3’ 脱靶序列的方法与传统的基于杂交的富集方法相比,有着更高的测序效率。这些经过修饰的目标序列随后被转化为含有唯一分子标签(UMI)及样本 barcode 的文库,与 Illumina 平台兼容。可用于上机测序的文库可以在一天之内制备完成。整个制备流程与大多数自动化仪器设备兼容。 NEBNext Direct HotSpot Cancer Panel 中的寡聚核苷酸饵能够捕获 50 个基因中 190 个常见癌症目标序列的双链 DNA,能够涵盖 40 kb 的序列,包括18,000 个COSMIC 数据库中所述情况。本 panel 适用于构建约 150 bp 的文库,适用于Illumina 平台 PE75 测序。 优势: 可比对到目标区域的reads 比例更高; 避免过度测序,减少每个样本的花费; 所有区域均可得到均一的测序结果,无论 GC 含量多少; 将富集和文库制备流程相结合,1 天即可完成; 能够从有限及降解的 DNA 样品中得到高质量的文库,包括 FFPE 及 ctDNA; 区分分子重复,降低假阳性突变并提高敏感度;该试剂盒的质控是独立管理的并通过功能验证。每个组分均通过标准的酶活性检测及质控,并通过严格的额外质量控制,质控情况可在每个组分的单独页面查询。
          Target enrichment, coupled with next generation sequencing (NGS), enables high throughput, deep sequencing of genomic regions of interest. NEBNext Direct is a novel, hybridization-based capture method offering significant advantages over traditional in-solution hybridization and multiplex PCR protocols. In the NEBNext Direct target enrichment approach (Figure 1), fragmented DNA is rapidly hybridized to biotinylated oligonucleotide baits that define the 3´ end of each target of interest. The bait-target hybrids are bound to streptavidin beads and any 3´ off target sequence is removed enzymatically. This combination of a short hybridization time with the enzymatic removal of 3´ off target sequence enables greater sequencing efficiency relative to conventional hybridization-based enrichment methods. The trimmed targets are then converted into Illumina-compatible libraries that include unique molecular identifiers (UMI) and a sample barcode. Sequence-ready libraries are generated within one day. The procedure is compatible with most automated liquid handling instruments. The NEBNext Direct HotSpot Cancer Panel contains baits that capture both strands of DNA across 190 common cancer targets from 50 genes, encompassing approximately 40 kb of sequence and including over 18,000 COSMIC features (Table 1). The panel is designed to generate targets of roughly 150 bp, compatible with PE75 Illumina sequencing. Advantages Generate a higher percentage of your sequencing reads aligning to your targets Eliminate the need to over-sequence, reducing cost per sample Obtain uniform sequencing of all targets, regardless of GC content Save time with a 1-day workflow that combines enrichment with library preparation Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA Distinguish molecular duplicates, reducing false positive variants and improving sensitivity
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